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排序方式: 共有293条查询结果,搜索用时 15 毫秒
81.
M van der Burg B Smit B Brinkhof B H Barendregt M C M Verschuren M Dib H B Beverloo J J M van Dongen A W Langerak 《Leukemia》2002,16(4):755-761
About 30% of T cell acute lymphoblastic leukemias (T-ALL) carry TAL1 gene aberrations. In the majority of cases (approximately 25%), this concerns a submicroscopic deletion of approximately 90 kb in chromosome region 1p32, which deletes the coding regions of the SIL gene and the untranslated region of the TAL1 gene, thereby placing the TAL1 gene under control of the SIL promoter region. Translocation (1;14)(p32;q11) involving the TAL1 gene occurs at a much lower frequency (3%), whereas some other rare variant translocations have been described as well. In this study we developed a set of TAL1 FISH probes based on the split-signal FISH principle that enables detection of both types of TAL1 gene aberrations in single test. For this purpose, one probe was designed downstream of the TAL1 gene (TAL1-D) and the second probe in the region upstream of the TAL1 gene, partly covering the SIL gene (SIL-U). We show that this split-signal FISH probe set allows reliable detection of the unaffected SIL-TAL1 gene region with a fusion signal, SIL-TAL1 fusion genes with loss of the SIL-U signal, and TAL1 gene translocations with a split-signal, independent of the involved partner gene. 相似文献
82.
A diagnosis of Hirschsprung's disease should be considered in children with constipation. An accurate neonatal history of bowel function and testing of anorectal pressure responses will aid the diagnosis. In the period 1971-75 inclusive, 140 children, aged 6 months to 14 years, were investigated by anorectal manometry. 26 showed a failed inhibition response to rectal dilatation, suggesting Hirschsprung's disease and were treated by anorectal myectomy. In 24 the disease was confirmed histologically. Two specimens were diagnostically unsuitable. 4 required repeat myectomies, and 3 anterior resection. At follow-up all had normal bowel movements without soiling. 相似文献
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Mutations of the alpha2(V) chain of type V collagen impair matrix assembly and produce ehlers-danlos syndrome type I 总被引:2,自引:0,他引:2
Michalickova K; Susic M; Willing MC; Wenstrup RJ; Cole WG 《Human molecular genetics》1998,7(2):249-255
Ehlers-Danlos syndrome (EDS) is a heterogeneous connective tissue disorder
that severely impairs the structure and function of the skin, joints, eyes
and blood vessels. We have identified mutations of the COL5A2 gene, which
encodes the alpha2(V) chain of type V collagen, in two unrelated patients
with the severe type I form of EDS. The first proband was heterozygous for
a 7 bp deletion that resulted in skipping of exon 27 while the second
proband was heterozygous for a single nucleotide substitution that resulted
in skipping of exon 28. Cultured dermal fibroblasts from both probands
produced about equal amounts of the normal and mutant alpha2(V) mRNAs and
protein chains. The dermis from the first proband contained a sparse
collagen fibrillar network with great variability in collagen fibril sizes
and shapes. The dermal collagens were also abnormally soluble. Bone cells
from the first proband also produced about equal amounts of the normal and
mutant alpha2(V) mRNAs. However, the collagen fibrillar architecture and
collagen solubility of the bone matrix were normal. Our findings show that
heterozygous mutations of the COL5A2 gene can produce the EDS type I
phenotype. They also suggest that type V collagen plays a more important
role in collagen fibrillogenesis of dermis than that of bone.
相似文献
86.
An enzyme-linked immunosorbent assay for detection of antibodies to porcine parvovirus 总被引:2,自引:0,他引:2
An enzyme-linked immunosorbent assay (ELISA) was developed for detection of antibodies to porcine parvovirus (PPV). Antisera to PPV were raised in pigs, for which PPV grown on PK15 cells was used for primary intranasal inoculations, and PPV cultured on autologous kidney cells for booster immunisations. A competition ELISA was developed, based on the principle of a double antibody sandwich assay, using immunoglobulin fractions prepared from these sera. The ELISA was compared with a haemagglutination inhibition (HI) test. The tests were equally sensitive for detecting antibodies early after infection and for detecting a significant increase in antibody titre between paired sera. A high correlation was found between antibody titres of field sera measured by the two tests (r = 0.91). We conclude that ELISA is preferable to the HI test, because it is labour-saving and can be standardised better and automated. 相似文献
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88.
Supratentorial ependymoma: CT appearance 总被引:5,自引:0,他引:5
Armington WG; Osborn AG; Cubberley DA; Harnsberger HR; Boyer R; Naidich TP; Sherry RG 《Radiology》1985,157(2):367-372
The computed tomographic appearances of 22 biopsy-proved supratentorial ependymomas were analyzed. Supratentorial ependymomas were usually intraparenchymal, larger than 4 cm, and cystic. Contrast enhancement was moderate to intense, with homogeneous or ring-enhancement patterns commonly seen. Intratumoral calcification was present in one-third of the cases, while hydrocephalus and peritumoral edema were seen in 50%. Intratumoral hemorrhage was not a characteristic of the lesion. In contrast to cellular ependymomas, malignant ependymomas and ependymoblastomas demonstrate higher attenuation prior to administration of contrast material, more intense enhancement, lower frequency of calcification within the tumor, and less distinct margination. 相似文献
89.