全文获取类型
收费全文 | 1419篇 |
免费 | 101篇 |
国内免费 | 3篇 |
专业分类
耳鼻咽喉 | 21篇 |
儿科学 | 39篇 |
妇产科学 | 5篇 |
基础医学 | 228篇 |
口腔科学 | 13篇 |
临床医学 | 125篇 |
内科学 | 295篇 |
皮肤病学 | 17篇 |
神经病学 | 66篇 |
特种医学 | 37篇 |
外科学 | 188篇 |
综合类 | 13篇 |
一般理论 | 2篇 |
预防医学 | 53篇 |
眼科学 | 71篇 |
药学 | 154篇 |
中国医学 | 12篇 |
肿瘤学 | 184篇 |
出版年
2023年 | 13篇 |
2022年 | 66篇 |
2021年 | 83篇 |
2020年 | 43篇 |
2019年 | 34篇 |
2018年 | 42篇 |
2017年 | 33篇 |
2016年 | 45篇 |
2015年 | 55篇 |
2014年 | 75篇 |
2013年 | 74篇 |
2012年 | 113篇 |
2011年 | 125篇 |
2010年 | 66篇 |
2009年 | 49篇 |
2008年 | 102篇 |
2007年 | 72篇 |
2006年 | 72篇 |
2005年 | 76篇 |
2004年 | 68篇 |
2003年 | 54篇 |
2002年 | 55篇 |
2001年 | 5篇 |
2000年 | 9篇 |
1999年 | 5篇 |
1998年 | 10篇 |
1997年 | 10篇 |
1996年 | 7篇 |
1995年 | 4篇 |
1994年 | 10篇 |
1993年 | 5篇 |
1992年 | 3篇 |
1991年 | 4篇 |
1990年 | 8篇 |
1989年 | 6篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1970年 | 1篇 |
排序方式: 共有1523条查询结果,搜索用时 15 毫秒
21.
Kun Sun Jeanette Br?ms Moa Lavander Bharat Kumar Gurram Per-Anders Enquist C. David Andersson Mikael Elofsson Anders Sj?stedt 《Antimicrobial agents and chemotherapy》2014,58(7):4123-4130
The type VI secretion system (T6SS) is the most prevalent bacterial secretion system and an important virulence mechanism utilized by Gram-negative bacteria, either to target eukaryotic cells or to combat other microbes. The components show much variability, but some appear essential for the function, and two homologues, denoted VipA and VipB in Vibrio cholerae, have been identified in all T6SSs described so far. Secretion is dependent on binding of an α-helical region of VipA to VipB, and in the absence of this binding, both components are degraded within minutes and secretion is ceased. The aim of the study was to investigate if this interaction could be blocked, and we hypothesized that such inhibition would lead to abrogation of T6S. A library of 9,600 small-molecule compounds was screened for their ability to block the binding of VipA-VipB in a bacterial two-hybrid system (B2H). After excluding compounds that showed cytotoxicity toward eukaryotic cells, that inhibited growth of Vibrio, or that inhibited an unrelated B2H interaction, 34 compounds were further investigated for effects on the T6SS-dependent secretion of hemolysin-coregulated protein (Hcp) or of phospholipase A1 activity. Two compounds, KS100 and KS200, showed intermediate or strong effects in both assays. Analogues were obtained, and compounds with potent inhibitory effects in the assays and desirable physicochemical properties as predicted by in silico analysis were identified. Since the compounds specifically target a virulence mechanism without affecting bacterial replication, they have the potential to mitigate the virulence with minimal risk for development of resistance. 相似文献
22.
Anju Sinha Nomita Chandhiok Seema Sahay Sibnath Deb Shalini Bharat Abhilasha Gupta 《AIDS care》2015,27(9):1196-1198
A compelling case for promoting male circumcision (MC) as an intervention for reducing the risk of heterosexually acquired HIV infection was made by dissemination of the results of three studies in Africa. The WHO/UNAIDS recommendation for MC for countries like India, where the epidemic in concentrated in high-risk groups, advocates MC for specific population groups such as men at higher risk for HIV acquisition. A multicentre qualitative study was conducted in four geographically distinct districts (Belgaum, Kolkata, Meerut and Mumbai) in India during June 2009 to June 2011. Two categories of health care providers: Registered Healthcare Providers (RHCPs) and traditional circumcisers were interviewed by trained research staff who had received master's level education using interview guides with probes and open-ended questions. Respondents were selected using purposive sampling. A comparative analysis of the perspectives of the RHCP vs. traditional circumcisers is presented. Representatives of both categories of providers expressed the need for Indian data on MC. Providers feared that promoting circumcision might jeopardize/undermine the progress already made in the field of condom promotion. Reservation was expressed regarding its adoption by Hindus. Behavioural disinhibition was perceived as an important limitation. A contrast in the practice of circumcision was apparent between the traditional and the trained providers. MC should be mentioned as a part of comprehensive HIV prevention services in India that includes HIV counselling and testing, condom distribution and diagnosis and treatment of sexually transmitted infections. It should become an issue of informed personal choice rather than ethnic identity. 相似文献
23.
24.
Sam M. Mbulaiteye Sheeja T. Pullarkat Bharat N. Nathwani Lawrence M. Weiss Nagesh Rao Benjamin Emmanuel Charles F. Lynch Brenda Hernandez Vishala Neppalli Debra Hawes Myles G. Cockburn Andre Kim Makeda Williams Sean Altekruse Kishor Bhatia Marc T. Goodman Wendy Cozen 《APMIS : acta pathologica, microbiologica, et immunologica Scandinavica》2014,122(1):5-15
Burkitt lymphoma (BL) occurs at all ages, but the patterns of Epstein–Barr virus (EBV) positivity in relation to human immunodeficiency virus (HIV), immunoprofiles and age have not been fully explored. BL tissues from residual tissue repositories, and two academic centers in the United States were examined by expert hematopathologists for morphology, immunohistochemistry, MYC rearrangement, EBV‐encoded RNA (EBER), and diagnosed according to the 2008 WHO lymphoma classification. Analysis was done using frequency tables, Chi‐squared statistics, and Student's t‐test. Of 117 cases examined, 91 were confirmed as BL. The age distribution was 26%, 15%, 19%, and 29% for 0–19, 20–34, 35–59, 60+ years, and missing in 11%. MYC rearrangement was found in 89% and EBER positivity in 29% of 82 cases with results. EBER positivity varied with age (from 13% in age group 0–19 to 55% in age group 20–34, and fell to 25% in age group 60+ years, p = 0.08); with race (56% in Blacks/Hispanics vs 21% in Whites/Asians/Pacific Islanders, p = 0.006); and by HIV status (64% in HIV positive vs 22% in HIV negative cases, p = 0.03). EBER positivity was demonstrated in about one‐third of tumors and it was strongly associated with race and HIV status, and marginally with age‐group. 相似文献
25.
26.
27.
Rajiv Bharat Kharwar M.D. Varun Shankar Narain M.D. D.M. Rishi Sethi M.D. D.M. F.A.C.C. 《Echocardiography (Mount Kisco, N.Y.)》2013,30(10):E331-E335
Aneurysms arising from the sinus of Valsalva are a rare cardiac defect that can present with various signs and symptoms, and if not diagnosed and treated rapidly can lead to fatal outcomes. Unruptured aneurysms are usually asymptomatic and found incidentally during diagnostic studies. More commonly, aneurysm of sinus of Valsalva is detected after the occurrence of rupture. Echocardiography has become the investigative tool of choice for this condition, not only for diagnosis but also for quantification of severity. We hereby report a rare case of a 15‐year‐old patient presenting with complaints of effort dyspnea and palpitations. Two‐dimensional transthoracic echocardiography (TTE) showed aneurysmal dilatation of left sinus of Valsalva which had ruptured into the left ventricle. Also, there was an intimal flap within the sinus of Valsalva aneurysm. The anatomical relationship between the aorta, aneurysm, and the left ventricle as well as the intimal flap within the aneurysm was clearly delineated with the help of three‐dimensional TTE. After confirmation of the diagnosis with multidetector computed tomography, patient underwent successful surgical repair of the defect. 相似文献
28.
A prospective study was carried out to assess the role of citric acid as a sole antimicrobial agent in the management of lepromatous ulcers. Thirty-four known cases of lepromatous ulcers not responding to conventional antibiotic therapies for long duration were investigated for culture and susceptibility studies. Staphylococcus aureus (25·00%) and Klebsiella spp. (23·43%) were found to be the most common isolates. Amikacin (68·75%) and ciprofloxacin (67·18%) were found to be the most effective antimicrobial agents. Topical application of citric acid ointment resulted in complete healing in 25 (73·52%) cases. In eight cases (26·48%), there was elimination of infective agent from ulcer site and formation of healthy granulation, but no complete healing of ulcer was seen. Results indicate that citric acid is the best alternative for the effective management of lepromatous ulcers when other therapies are exhausted. 相似文献
29.
30.