牙骨质基质提取物促进牙龈成纤维细胞在光滑钛表面上粘附及移行的实验研究

目的 :了解牙骨质基质提取物能否促进牙龈成纤维细胞在金属钛表面的粘附与移行 ,以改善种植体的龈界面。方法 :选用表面光滑的金属钛片作为细胞粘附的底物 ,检测各时间点牙骨质基质提取物对牙龈成纤维细胞在钛片上贴壁率的影响 ,并着运用图象分析法分析 1、3、6天钛片周围牙龈成纤维细胞的粘附指数和 3、6天钛片周围牙龈成纤维细胞的移行指数。结果 :随牙骨质基质提取物作用时间的延长牙龈成纤维细胞的贴壁率不断上升 ,且牙骨质基质提取物无论是牙龈成纤维细胞在钛片周围的粘附指数还是移行指数均明显高于对照组。结论 :牙骨基质提取物可作为种植体表面的生物涂层材料以在种植体周围获得良好的牙龈封闭。     

大鼠丘脑束旁核的电镜观察

透射电镜下观察了大鼠ＰＦ中参与突触构成的各种轴突终末及突触联系为主的超微结构特征，ＰＦ中参与突触构成的轴突终末分为Ｒ，Ｆ，Ｐ及Ｇ终末四类，Ｒ终末包括ＳＲ及Ｇ终末四类，Ｒ终末包括ＳＲ及ＬＲ终末，ＳＲ终末占８１．５３％，为含有小圆形清亮小泡的轴突终末，所含小泡平均直径为４３ｎｍ，ＬＲ终末占９４．５％，含较大直径的圆形清亮小泡，平均直径为５６ｎｍ，与树突构成对称或不对称的轴－树突触，Ｆ终末占６．４４％，     

60例晚发月经初潮女性的手腕骨发育特征

对60例14周岁之后发生月经初潮的女生进行了手腕骨发育特征观察，其平均骨龄为13．55岁，标准差为0．46岁，平均时间年龄为15．01岁。可见骨龄落后于时间年龄，骨皮质发育也落后；75％的观察对象第Ⅱ指末节指骨干骺完全融合。     

兔急性感染性脑水肿脑组织钙含量的改变

采用百日咳菌液右侧颈内动脉注射造成兔急性感染性脑水肿模型，测定脑组织含水量和含钙量。结果显示：注射菌液后１ｈ，右侧脑组织含水量和含钙量较对照组明显升高，并随病情发展进一步增高，两者变化呈显著正相关；左侧脑组织含水量增高前２ｈ已有明显钙聚积。提示脑组织钙聚积可能系感染性脑水肿发生、发展的重要因素之一。     

3种抗缺氧药物对筑路工人急进海拔4 600m不同时间血红蛋白的影响

目的:探讨三种抗缺氧药物对筑路工人急进高海拔地区不同时间血红蛋白的影响.方法:受试者随机分为服药组A组(复方1号)、B组(复方2号)、C组(复方3号)和对照组(D组),每组(23～24)人.启程时开始服药,每天早、中、晚各服药一次,连续服药3周.到达海拔4 600m施工现场第1天、3天、7天、15天进行血红蛋白测定,与服药前血红蛋白比较.结果:进驻后第一天各组血红蛋白与服药前组内比较,差别有非常显著性(P＜0.01);第15天复方2号组、安慰剂组与服药前组内比较,差别有显著性(P＜0.05).第1天与第3天、第15天各组组内比较,差别有显著性(P＜0.05);第一天与第7天比较,除复方2号组外其余各组组内比较,均差别有非常显著性(P＜0.01);第3天与第15天复方1号组、复方3号组组内比较,差别有显著性(P＜0.05).结论:三种抗缺氧药物均不同程度改善红细胞携氧功能,以复方1号、复方3号效果较好.     

陈旧性心肌梗塞的体表等电位图特征

用体表电位标测微机系统(Ⅰ型)检测了66例陈旧性心肌梗塞患者的体表等电位图。发现心肌梗塞QRS的体表等电位图变化主要在心室兴奋的早、中期,相当于心电图上的Q波期;前间壁、广泛前壁和局限性前壁心肌梗塞时,前胸部反映出电兴奋丧失,呈负区;一般坏死范围越大,负区范围也越广,负压值越大。前壁梗塞时,电位图变化主要在心室兴奋的前42ms,晚期图形基本不变;下壁梗塞的,在心室兴奋的早期(18ms)以前,负压在前胸及背部下区,中期多反映在前胸。因此,根据不同梗塞部位及左室不同兴奋时间的体表等电位变化特征,可以诊断出常规胸导心电图正常的心肌梗塞患者。     

Rho GDP dissociation inhibitor protects cancer cells against drug-induced apoptosis

Rho GDP dissociation inhibitor (RhoGDI) plays an essential role in control of a variety of cellular functions through interactions with Rho family GTPases, including Rac1, Cdc42, and RhoA. RhoGDI is frequently overexpressed in human tumors and chemo-resistant cancer cell lines, raising the possibility that RhoGDI might play a role in the development of drug resistance in cancer cells. We found that overexpression of RhoGDI increased resistance of cancer cells (MDA-MB-231 human breast cancer cells and JLP-119 lymphoma cells) to the induction of apoptosis by two chemotherapeutic agents: etoposide and doxorubicin. Conversely, silencing of RhoGDI expression by DNA vector-mediated RNA interference (small interfering RNA) sensitized MDA-MB-231 cells to drug-induced apoptosis. Resistance to apoptosis was restored by reintroduction of RhoGDI protein expression. The mechanism for the anti-apoptotic activity of RhoGDI may derive from its ability to inhibit caspase-mediated cleavage of Rac1 GTPase, which is required for maximal apoptosis to occur in response to cytotoxic drugs. Taken together, the data show that RhoGDI is an anti-apoptotic molecule that mediates cellular resistance to these chemotherapy agents.     

坐骨神经选择性损伤痛模型小鼠脊髓背角线粒体解偶连蛋白UCP4的表达变化

目的:观察线粒体保护蛋白解偶联蛋白4(uncoupling protein 4,UCP4)在坐骨神经选择性损伤(sparednerve injury,SNI)模型小鼠脊髓背角中的表达变化。方法:健康C57BL/6小鼠分为假手术对照组(n=21)和坐骨神经分支选择性损伤SNI组(n=21),实验组损伤后饲养3,7,14 d。行为学采用测定小鼠热痛阈和Von Frey机械性痛阈;用免疫荧光组织化学染色法检测对比小鼠脊髓L3-6节段背角内UCP4免疫阳性细胞的数量。结果:SNI术后3 d,小鼠手术侧热痛阈和机械性痛阈明显低于假手术组,术后14 d达最低值。UCP4分布于正常小鼠脊髓背角,SNI后3 d损伤组小鼠脊髓背角中的UCP4表达降低,图像分析表明UCP4的光密度与对照组比较,差异有统计学意义(P<0.05);脊髓背角中UCP4的表达在14 d时其降低程度最明显,图像分析表明光密度与对照组、3d和7 d比较,差异均有统计学意义(P<0.05)。结论:SNI后脊髓背角线粒体保护蛋白UCP4表达降低可能参与神经病理性疼痛的中枢敏化过程。     

《哲对宁诺尔》所载治疗皮肤病方剂的特点

《哲对宁诺尔》是蒙医经典著作之一,收载了许多经典方剂。有的至今仍是蒙医临床常用方剂。收载的治疗皮肤病方剂中,有内服的,也有外用的,有的已经很少使用或不再使用。这些方剂的组方特点是:恰与皮肤病的治疗原则相吻合,具有开发、研究、应用价值。     

Unrelated cord blood transplantation for central nervous system relapse in high-risk childhood acute lymphoblastic leukemia

Few clinical studies have investigated the role of unrelated cord blood transplantation (CBT) for central nervous system (CNS) relapse of childhood acute lymphoblastic leukemia (ALL) patients with high-risk factors. The aim of this report is to identify the potential benefits of unrelated CBT in high-risk childhood ALL with CNS relapse who has been treated on CNS-directed treatment strategies. Eleven childhood ALL patients with CNS relapse who underwent unrelated CBT enrolled in our study between 2001 and 2011, and all of the patients had features associated with poor outcomes, such as high white blood cells at diagnosis, ph?+?chromosome, or a history of bone marrow relapse. All transplants were performed with myeloablative-conditioning therapy (BU/cyclophosphamide (CY₂) or total body irradiation/CY) plus highly CNS-active agents (carmustine or high-dose cytarabine). All patients achieved neutrophil engraftment and platelet engraftment. A total of nine patients (81.8 %) developed pre-engraftment syndrome at a median of 7 days, and three patients developed acute graft-vs-host disease at a median of 21 days. The median follow-up after CBT was 28.5 months. The probability of overall survival at 9 years was 63.6 %, and no patient experienced a CNS relapse. Our experience suggests that unrelated CBT appears to be an effective treatment option for CNS relapse of childhood ALL patients associated with poor outcome features.