Synthesis of heteroatom-containing pyrrolidine derivatives based on Ti(O-iPr)4 and EtMgBr-catalyzed carbocyclization of allylpropargyl amines with Et2Zn

The Ti(O-iPr)₄ and EtMgBr-catalyzed regio and stereoselective carbocyclization of N-allyl-substituted 2-alkynylamines with Et₂Zn, followed by deuterolysis or hydrolysis, affords the corresponding methylenepyrrolidine derivatives in high yields. It was found that Ti–Mg-catalyzed carbocyclization of N-allyl-substituted 2-alkynylamines with Et₂Zn is equally selective in dichloromethane, hexane, toluene, and diethyl ether. The reaction was tolerant to the presence of aryl, alkyl, trimethylsilyl, methoxymethyl and aminomethyl substituents on the alkyne. A selective method was proposed for the preparation of bis-pyrrolidine derivatives using Ti–Mg-catalyzed carbocyclization of bis-allylpropargyl amines with Et₂Zn.

The Ti(O-iPr)₄ and EtMgBr-catalyzed regio and stereoselective carbocyclization of N-allyl-substituted 2-alkynylamines with Et₂Zn, followed by deuterolysis or hydrolysis, affords the corresponding methylenepyrrolidine derivatives in high yields.     

新疆出血热病毒免疫荧光试验检测结果报告

目的了解新疆出血热(XHF)的自然界分布状况。方法对采自塔里木盆地、准噶尔盆地、伊犁谷地和东疆盆地23个县(市)的96份病原材料分别接种Vero-E6细胞,病原材料经细胞培养后,胰酶消化制备细胞片,应用免疫荧光试验(IFA)对细胞片进行病毒检测。结果96份病原材料中,检出新疆出血热病毒颗粒阳性材料16份,分布地区包括巴楚、于田、轮台、民丰、尉梨、莫索湾、吐鲁番和木垒。结论巴楚、于田、轮台、尉梨、莫索湾和木垒地区存在新疆出血热的自然界分布,而民丰和吐鲁番仅从羊血液标本中查出阳性,不能说明该地区存在新疆出血热的自然界分布。     

Effects of ATP and adenosine on contraction amplitude of rat soleus muscle at different temperatures

Introduction: The aim of this study was to evaluate the effects of adenosine 5′‐triphosphate (ATP) and adenosine on the contractility of mammalian skeletal muscle under hypothermic conditions. Methods: Contractions of isolated rat soleus muscle were induced by either electrical stimulation (ES) or carbachol at physiological temperatures (37°C) and hypothermic conditions (30–14°C) and recorded in the presence of ATP, adenosine, suramin, and 8‐(p‐sulfophenyl)‐theophylline (8‐SPT). Results: At 37°C, incubation of the muscles with ATP inhibited ES‐induced contractions; the inhibitory effect of ATP disappeared at 14°C. Adenosine inhibited ES‐induced contractions at all temperature levels; 8‐SPT fully prevented the action of adenosine. ATP and adenosine did not significantly affect carbachol‐induced contractions at 37°C, while at lower temperatures ATP potentiated them. Suramin fully prevented effects of ATP. Conclusions: ATP is involved in both pre‐ and postsynaptic regulation of rat soleus muscle contractility, and these processes are significantly more pronounced at low temperatures. Muscle Nerve 55 : 417–423, 2017     

In vivo and in vitro tracking of bone marrow mesenchymal stem cells delivered to infarcted myocardium by superparamagnetic iron oxide nanoparticles

背景：活体示踪移植干细胞,影像学评估移植细胞动态变化的趋势是目前的热点研究方向。 目的：探讨MRI活体示踪超顺磁性氧化铁标记的骨髓间充质干细胞移植修复心肌的可行性及有效性。 方法：经导管应用球囊封堵前降支动脉建立家猪心肌梗死模型,造模成功的动物随机分为2组：实验组在造模2周后于梗死区中央及梗死边缘区共5个点注射超顺磁性氧化铁-多聚赖氨酸混合物标记的骨髓间充质干细胞悬液1 mL,对照组以同样方法注入等量的生理盐水。 结果与结论：细胞移植后1 h,2周,实验组中4只动物(100%)移植区在磁共振上呈明显的低信号改变,移植后4周示3只动物(75%)细胞移植区有低信号改变。移植后4周,心肌组织荧光免疫组化检测心脏结蛋白、心脏连接蛋白和心肌早期转录蛋白均有表达。结果可见应用MRI技术活体示踪动态观察移植的骨髓间充质干细胞是可行有效的。     

Niobium- and zirconium-catalyzed reactions of substituted 2 alkynylamines with Et2Zn

The NbCl₅–EtMgBr-catalyzed reaction of N,N-disubstituted 2-alkynylamines with Et₂Zn followed by hydrolysis or deuterolysis affords (2Z)-alkenylamines (reduction products of alkyne) in high yields. The reaction of N,N-disubstituted 2-alkynylamines with Et₂Zn catalyzed by the Cp₂ZrCl₂–EtMgBr system occurs as 2-zincoethylzincation, resulting, after deuterolysis or iodinolysis, in the regio- and stereoselective formation of the corresponding dideuterated and diiodinated 2-alkenylamine derivatives with a trisubstituted double bond. This study demonstrates the difference between the catalytic effects of NbCl₅ and Cp₂ZrCl₂ on the pathway of reaction of tertiary 2-alkynylamines with Et₂Zn in the presence of catalytic amounts of EtMgBr.

The NbCl₅–EtMgBr-catalyzed reaction of N,N-disubstituted 2-alkynylamines with Et₂Zn followed by hydrolysis or deuterolysis affords (2Z)-alkenylamines (reduction products of alkyne) in high yields.     

超顺磁性氧化铁标记骨髓间充质干细胞心肌活体示踪及体外实验☆

背景:活体示踪移植干细胞,影像学评估移植细胞动态变化的趋势是目前的热点研究方向。目的:探讨MRI活体示踪超顺磁性氧化铁标记的骨髓间充质干细胞移植修复心肌的可行性及有效性。方法:经导管应用球囊封堵前降支动脉建立家猪心肌梗死模型,造模成功的动物随机分为2组:实验组在造模2周后于梗死区中央及梗死边缘区共5个点注射超顺磁性氧化铁-多聚赖氨酸混合物标记的骨髓间充质干细胞悬液1mL,对照组以同样方法注入等量的生理盐水。结果与结论:细胞移植后1h,2周,实验组中4只动物(100%)移植区在磁共振上呈明显的低信号改变,移植后4周示3只动物(75%)细胞移植区有低信号改变。移植后4周,心肌组织荧光免疫组化检测心脏结蛋白、心脏连接蛋白和心肌早期转录蛋白均有表达。结果可见应用MRI技术活体示踪动态观察移植的骨髓间充质干细胞是可行有效的。