Electromyography and Fos immunostaining study establish a possible functional link between trigeminal proprioception and the oculomotor system in rats

The objective of this study was to explore whether there was a functional link between trigeminal proprioception and the oculomotor system mediated through jaw muscle afferents. Electromyography (EMG) was undertaken of the levator palpebrae (LP) and superior rectus (SR), and Fos expression was detected in the brainstem following consecutive down-stretching of the lower jaw at 2-4 Hz in rats. Retrograde tracing was undertaken of the interstitial nucleus of Cajal and Darkschewitsch nucleus (INC/DN) pre-oculomotor neurons. EMG-like responses were recorded from the LP/SR during down-stretching of the lower jaw at 2-4 Hz in 3 out of 11 rats. Fos expression was induced by consecutive down-stretching of the lower jaw at 2-4 Hz for 20-30 seconds. Interestingly, Fos expression was distributed mainly in the bilateral INC/DN area. We also examined Fos-like immunoreactivity in central mesencephalic and paramedian pontine reticular formation that harbors premotor neurons controlling horizontal eye movement, but no Fos-like staining was observed therein. By injection of retrograde tracers into the oculomotor nucleus combined with Fos immunostaining, double labeled pre-oculomotor neurons were visualized to distribute in the INC/DN. In conclusions, there may exist a trigeminal proprioceptive – oculomotor system neural circuit through jaw muscle afferents in rats. Judging from Fos distribution pattern, this pathway might be related to vertical and torsional eye movements.     

阴式子宫切除术联合阴道前后壁修补术对子宫脱垂合并阴道壁膨出患者术后疼痛及复发的影响

目的探讨阴式子宫切除术联合阴道前后壁修补术对子宫脱垂合并阴道壁膨出患者术后疼痛及复发的影响。 方法选取2017年1月到2019年1月,安徽省马鞍山市中心医院收治的90例子宫脱垂合并阴道壁膨出患者。采用随机数字表法将其分为对照组和观察组。对照组45例采用单纯阴道前后壁修补术进行治疗,观察组45例采用阴式子宫切除术联合阴道前后壁修补术进行治疗,2组术后随访1年。比较2组治疗后的临床疗效及手术前后视觉模拟评分（VAS）;统计2组围手术期手术相关指标及并发症发生率和随访1年的复发率。采用SPSS 21.0统计软件进行数据分析。 结果观察组总有效率为93.33%,显著高于对照组的71.11%,差异有统计学意义（P<0.05）。与手术前相比,术后3～7 d,2组VAS评分均呈逐渐降低趋势,且术后3、7 d观察组显著低于对照组,差异有统计学意义（P<0.05）。与对照组相比,观察组术中出血量明显较少,差异有统计学意义（P<0.05）;观察组的住院时间、肛门排气时间、手术时间等明显较短,差异有统计学意义（P<0.05）。观察组并发症发生率、随访1年复发率分别为6.67%、2.22%,显著低于对照组的26.67%、24.44%,差异有统计学意义（P<0.05）。 结论阴式子宫切除术联合阴道前后壁修补术治疗子宫脱垂合并阴道壁膨出,可显著改善患者围手术期相关指标的情况,减轻患者术后疼痛,并能降低患者并发症发生率及术后复发率,临床疗效显著。     

急性肢体动脉闭塞的血管内介入治疗

目的 探讨急性肢体动脉闭塞的血管内治疗方法和疗效。方法 采用血管内尿激酶溶栓术和经皮穿刺血管成形术（ＰＴＡ）治疗患者１２例１９段。男性６例、女性６例,年龄２８～８５岁,平均６５岁。结果 溶栓治疗成功率６８．４２（１３／１９段）,ＰＴＡ治疗成功率９４．１２（１６／１７段）。观察６个月以上疗效满意率为８４．２１％（１６／１９段）。结论 局部溶栓是治疗急性肢体动脉闭塞的基本方法,大部分病例还需ＰＴＡ治疗     

PALB2 upregulation is associated with a poor prognosis in pancreatic ductal adenocarcinoma

During DNA repair, BRCA1 and BRCA2 interact with the tumor suppressor partner and localizer of BRCA2 (PALB2). PALB2 mutations are associated with an increased risk of breast and ovarian carcinoma, and upregulated PALB2 expression is associated with poor clinical outcomes. The present study investigated the role and prognostic value of PALB2 in pancreatic ductal adenocarcinoma (PDAC). PALB2 expression was inhibited using a small interfering RNA in PDAC cell lines, and the subsequent effects on cell proliferation and migration were investigated. Tissue microarrays from 157 patients undergoing a pancreaticoduodenectomy for PDAC were analyzed via immunohistochemistry, and PALB2 expression was compared with patient outcomes using Kaplan-Meier curves and the multivariate Cox regression model. PALB2-knockdown in PDAC cells had little effect on cell proliferation, but significantly decreased cell migration. Relatively high PALB2 expression was observed in PDAC tissues compared with in peritumoral tissues. Overall survival (OS) was negatively associated with PALB2 expression. TNM stage and PALB2 expression were identified as independent prognostic factors associated with OS via multivariate analysis. Overall, the present study demonstrated that PDAC cell migration was dependent on PALB2, which was further supported by the finding that elevated PALB2 expression in PDAC tissues was associated with poor survival in patients with PDAC. Therefore, PALB2 may serve as a novel prognostic marker in PDAC, which may aid with the development of therapeutic strategies for the disease.     

高风险注射制剂标识方式的帕累托法则分析及标准化研究

目的 为高风险药品标识的标准化标注提供参考。方法 按样本医院住院药房高风险药品目录,选取高风险注射制剂单独设置药品目录,按第17版《新编药物学》中药品的药理学分类分析其品种分布。结合不同高风险注射制剂的材质、体积及高风险标识在日常工作中的粘贴方式,利用帕累托法则探讨高风险标识的标准化标注改进措施。结果 与结论样本医院住院药房共有46种高风险注射制剂,其中43种(93.48%)药品包装材质为玻璃,且30种(65.22%)为安瓿瓶,13种(28.26%)为西林瓶;注射液体积按供应种类数量排序从高到低依次为2,1,10,5,20 m L,其品种数占比累计在80%以内,均为安瓿瓶;瓶身大小统一,可实现高风险药品的标准化标注。     

浅谈武警部队疗养院移动救护平台的应用与意义

武警部队疗养院担负着全部队师以上干部、艰苦地区支队团主官和基层英模代表的疗养任务,为维护和提高部队战斗力服务。近年来,疗养员呈现老龄化趋势,患老     

ContourSe微球联合碘油栓塞治疗大肝癌的初步临床研究

目的：探讨ContourSe微球联合碘油动脉栓塞治疗大肝癌的临床应用价值。方法：19例大肝癌，其中常规肝动脉化疗栓塞（TACE）治疗碘油廓清快的病例13例，初次治疗6例。常规动脉造影后行化疗栓塞。根据所用栓塞剂的不同分为2组，即（1）常规栓塞组（10例）：在肿瘤供血动脉灌注化疗药物后，注入超液化碘油和表柔比星制成的乳剂，然后用明胶海绵条栓塞供血动脉；（2）微球碘油栓塞组（9例）：微球作为辅助栓塞剂，超选择插管灌注化疗后，注入超液化碘油与ContourSe微球及表柔比星混合制成的混悬剂，供血动脉近端用明胶海绵栓塞。CT随访肿瘤大小变化，肿瘤坏死程度，碘油沉积情况；对比不同栓塞方法对肝功能的影响；栓塞前后AFP变化以及并发症发生情况。结果：常规栓塞组术后肿瘤缩小3例，无变化5例，增大2例；微球碘油栓塞组术后肿瘤缩小6例，无变化1例，增大2例，其中2例部分坏死，3例近完全坏死。微球碘油栓塞治疗与常规栓塞治疗对肝功能的影响无显著差别；微球碘油栓塞组术前及术后1个月肝功能无显著差别；微球碘油栓塞组术后AFP下降5例，发生肝脓肿1例，无其他严重并发症。结论：应用ContourSe微球作为辅助栓塞剂对大肝癌进行化疗栓塞安全可行，近期效果良好，尤其适用于大肝癌的首次介入治疗。     

反复妊娠失败患者抑郁、焦虑发病率及其影响因素分析

目的探讨反复妊娠失败患者抑郁、焦虑发病率及其相关影响因素。方法选取2019年1月至12月于温州医科大学附属康宁医院妇产科门诊就诊的113例反复妊娠失败患者作为研究对象,采用焦虑自评量表(SAS)和抑郁自评量表(SDS)评估心理状况,影响因素分析采用多因素Logistic回归。结果113例患者中,焦虑发生率55.75%(63/113),抑郁发生率59.29%(67/113);多因素Logistic回归分析显示,年龄、妊娠失败次数、家庭年收入、夫妻关系及医护指导是反复妊娠失败患者焦虑的影响因素,其OR值及95%CI分别为1.992(1.204~5.006)、2.148(1.367~7.467)、0.675(0.367~0.851)、0.606(0.344~0.849)、0.634(0.462~0.907),均P<0.05;妊娠失败次数、家庭年收入、家人照顾、夫妻关系及医护指导是反复妊娠失败患者抑郁的影响因素,其OR值及95%CI分别为2.241(1.545~6.886)、0.458(0.267~0.738)、0.677(0.416~0.884)、0.573(0.361~0.783)、0.544(0.409~0.866),均P<0.05。结论反复妊娠失败患者中焦虑、抑郁现象明显,其与妊娠失败次数、家庭年收入、夫妻关系、医护指导存在相关性。     

浅谈武警部队疗养院服务模式现状及发展

<正>武警部队疗养院是武警部队卫生医疗机构的重要组成部分,近年来疗养院建设在深度和广度上有了明显发展。然而,武警部队疗养院建院时间较短,还没有形成完整配套的保障体系。与军队同类疗养院相比,综合效益不     

上海某儿童医院住院患者分剂量口服高风险片剂的使用调查

摘 要 目的：调查儿童医院住院患者高风险片剂的使用剂量及频率分布,研究现有高风险药品规格是否满足儿科临床需求。方法: 调取2014～2016年全年上海儿童医学中心住院患者高风险片剂全部处方,分析各药品各剂量的使用频率,依据频率判断现有规格是否满足临床实际需求。对不满足临床实际需求的药品给出建议新增规格,依据建议的规格重新核算3年间全部处方,给出核算后的使用频率,以此来评价建议新增的规格是否贴合临床实际需求。结果:该医院有5种高风险口服片剂,甲氨蝶呤片和维A酸片现有规格符合临床实际需求。巯嘌呤片需要新增12.5 mg和17 mg两种规格品种,华法林钠片需要新增1.25 mg品种。羟基脲片250 mg和华法林钠片1 mg品种建议引入儿童医院。结论:现有高风险口服片剂剂量规格并不能完全满足临床需求,需要进行剂量调整。