全文获取类型
收费全文 | 242篇 |
免费 | 9篇 |
专业分类
耳鼻咽喉 | 5篇 |
妇产科学 | 12篇 |
基础医学 | 39篇 |
口腔科学 | 1篇 |
临床医学 | 10篇 |
内科学 | 25篇 |
皮肤病学 | 18篇 |
神经病学 | 25篇 |
特种医学 | 41篇 |
外科学 | 17篇 |
综合类 | 1篇 |
预防医学 | 25篇 |
眼科学 | 7篇 |
药学 | 11篇 |
肿瘤学 | 14篇 |
出版年
2024年 | 1篇 |
2023年 | 3篇 |
2022年 | 2篇 |
2021年 | 7篇 |
2020年 | 4篇 |
2019年 | 6篇 |
2018年 | 7篇 |
2017年 | 8篇 |
2016年 | 5篇 |
2015年 | 12篇 |
2014年 | 14篇 |
2013年 | 12篇 |
2012年 | 22篇 |
2011年 | 17篇 |
2010年 | 10篇 |
2009年 | 6篇 |
2008年 | 9篇 |
2007年 | 13篇 |
2006年 | 18篇 |
2005年 | 7篇 |
2004年 | 10篇 |
2003年 | 8篇 |
2002年 | 5篇 |
2001年 | 1篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1985年 | 1篇 |
1983年 | 1篇 |
1982年 | 3篇 |
1981年 | 2篇 |
1979年 | 2篇 |
1978年 | 1篇 |
1977年 | 5篇 |
1976年 | 3篇 |
1975年 | 3篇 |
1974年 | 1篇 |
1968年 | 1篇 |
1967年 | 1篇 |
1966年 | 1篇 |
1940年 | 2篇 |
1938年 | 2篇 |
1937年 | 2篇 |
1934年 | 2篇 |
1933年 | 3篇 |
1924年 | 1篇 |
1915年 | 1篇 |
排序方式: 共有251条查询结果,搜索用时 15 毫秒
81.
Sequencing of approximately one half of the genome of acipenserid herpesvirus 2 (AciHV-2), which is a member of the genus
Ictalurivirus in the family Alloherpesviridae, revealed that the gene organization is very similar to that of ictalurid herpesvirus 1 (IcHV-1), the founder member of the
genus. The sequenced region encodes the AciHV-2 homologues of IcHV-1 ORF24 to ORF69. It contains 46 predicted protein-coding
regions, including 12 that seem to have a homologue in every alloherpesvirus genome sequenced to date. Phylogenetic tree reconstruction,
based on the concatenated sequence of these conserved genes, implied that the family Alloherpesviridae is composed of three major clades and could be subdivided into three subfamilies. 相似文献
82.
83.
84.
Kranenburg AR Willems-Widyastuti A Moori WJ Sterk PJ Alagappan VK de Boer WI Sharma HS 《American journal of clinical pathology》2006,126(5):725-735
Remodeling of airways and blood vessels is an important feature in chronic obstructive pulmonary disease (COPD). By using immunohistochemical analysis, we examined bronchial expression patterns of various extracellular matrix (ECM) components such as collagens (subtypes I, III, and IV), fibronectin, and laminin beta2 in patients with COPD (forced expiratory volume in 1 second [FEV1] or=85%; n = 16) and correlated expression data with lung function. Quantitative analysis revealed enhanced levels (P < .01) of total collagens I, III, and IV in surface epithelial basement membrane (SEBM) and collagens I and III in bronchial lamina propria (P < .02) and adventitia (P < .05) in COPD. Distinct and increased (P < .05) vascular expression of fibronectin accounts for intimal vascular fibrosis, whereas laminin beta2 (P < .05) was elevated in airway smooth muscle (ASM). FEV1 values inversely correlated with collagens in the SEBM, fibronectin in bronchial vessels, and laminin in the ASM. Our data suggest that COPD exhibits increased bronchial deposition of ECM proteins that contribute to deteriorated lung function and airway remodeling. 相似文献
85.
86.
87.
Morsal?SamimEmail author Linde?M.?van?Veenendaal Manon?N.?G.?J.?A.?Braat Andor?F.?van den?Hoven Richard?Van?Hillegersberg Bruno?Sangro Yung?Hsiang?Kao Dave?Liu John?D.?Louie Daniel?Y.?Sze Steven?C.?Rose Daniel?B.?Brown Hojjat?Ahmadzadehfar Edward?Kim Maurice?A.?A.?J.?van den?Bosch Marnix?G.?E.?H.?Lam 《European radiology》2017,27(12):4923-4930
88.
Andor?F.?van den?HovenEmail author Manon?N.?G.?J.?A.?Braat Jip?F.?Prince Pieter?J.?van?Doormaal Maarten?S.?van?Leeuwen Marnix?G.?E.?H.?Lam Maurice?A.?A.?J.?van den?Bosch 《European radiology》2017,27(1):61-69
Objectives
To compare right gastric (RGA) and segment 4 artery (A4) origin detection rates during radioembolisation workup between early and late arterial phase liver CT protocols.Methods
100 consecutive patients who underwent liver CT between May 2012–January 2015 with early or late arterial phase protocol (n?=?50 each, 10- vs. 20-s post-threshold delay) were included. RGA/A4 origin detection rates, assessed by two raters, and contrast-to-noise ratio (CNR) of the hepatic artery relative to the portal vein were compared between the protocols.Results
The first–second rater scored the RGA origin as visible in 58–65 % (specific proportion of agreement 82 %, κ?=?0.62); A4 origin in 96–89 % (94 %, κ?=?0.54). Thirty-six percent of RGA origins not detectable by DSA were identified on CT. Origin detection rates were not significantly different for early/late arterial phases. Mean CNR was higher in the early arterial phase protocol (1.7 vs. 1.2, p?<?0.001).Conclusion
A 10-s delay arterial phase CT protocol does not significantly improve detection of small intra- and extrahepatic branches. RGA origin detection requires further optimization, whereas A4/MHA origin detection is adequate, with good inter-rater reproducibility. CT remains important for preprocedural planning, because it may reveal arterial anatomy not discernible on DSA.Key Points
? An early arterial phase does not significantly improve RGA and A4/MHA origin detection. ? RGA origin detection (58–65 %) on CT is still suboptimal. ? 36 % of RGA origins undetectable on DSA can be identified on CT. ? A4/MHA origin detection (89–96 %) on CT is excellent. ? Inter-rater reproducibility is good for RGA and A4/MHA origin detection on CT.89.
Belsõ N Széll M Pivarcsi A Kis K Kormos B Kenderessy AS Dobozy A Kemény L Bata-Csörgõ Z 《The Journal of investigative dermatology》2008,128(3):634-642
In this study, we show that the G0-G1/S phase of HaCaT keratinocyte cell cycle is characterized by D1-type cyclin expression, whereas during the repeated rapid turnover of highly proliferating cells, the expression of cyclins D2 and D3 dominates. Knocking down cyclin D1 mRNA resulted in no change of cell proliferation and morphology, indicating that D2 and D3 cyclins could substitute for D1 in driving the cell cycle. Increased numbers of cyclin D1-expressing keratinocytes were found in the basal layers of the lesional psoriatic epidermis compared to both normal and non-lesional epidermis without increased expression of cyclin D1 mRNA, suggesting a possible dysfunction in the degradation of cyclin D1 protein. We also detected a significant increase in cyclin D2 and D3 mRNA expressions in psoriatic epidermis compared to normal epidermis with no difference in protein expressions. Blocking alpha5-integrin function by a neutralizing antibody in HaCaT keratinocytes downregulated the expression of cyclin D1 mRNA without affecting the expressions of cyclin D2 and D3 indicating a regulatory role for alpha5-integrin in the expression of cyclin D1. Our data suggest a possible role for D-type cyclins in the excessive basal-cell proliferation and perturbed keratinocyte differentiation in the psoriatic epidermis. 相似文献
90.
Charbel Bouez Michela Zuccolo Audrey Gueniche Mona Ståhle Enikö Sonkoly Lionel Breton Andor Pivarcsi 《Experimental dermatology》2014,23(4):281-283
Keratinocytes recognize invading pathogens by various receptors, among them Toll‐like receptors (TLRs), and provide the first line of defense in skin immunity. The role of microRNAs in this important defense mechanism has not been explored yet. Our aim was to identify microRNAs involved in the innate immune response of keratinocytes. MicroRNA expression profiling revealed that the TLR2 ligand zymosan, the TLR3 ligand poly(I:C) or the TLR5 ligand flagellin significantly altered the microRNA expression in keratinocytes. The regulation of microRNAs was concentration‐dependent and it could be neutralized by siRNAs specific for TLR2, TLR3 and TLR5, respectively, confirming the specificity of the TLR response. Interestingly, one microRNA, miR‐146a, was strongly induced by all studied TLR ligands, while other microRNAs were regulated in a TLR‐ or time point‐specific manner. These findings suggest an important role for microRNAs in the innate immune response of keratinocytes and provide a basis for further investigations. 相似文献