山西省医疗机构传染病疫情管理现状调查分析

为了解我省各级医疗机构传染病疫情管理状况 ,在 2 0 0 0年全省法定传染病漏报调查的同时 ,对调查县 (区、市 )所辖医疗机构的传染病疫情管理现状进行了调查 ,调查情况报告如下。1 设立防保组织基本情况 :全省 1 1个地、市的 35个县 (区市 )共调查了 731所各级各类医疗机构 ,其中 5 81所医疗机构设有防保科 ,占调查机构的 79 48% ;5 5 3个防保科能开展防保工作 ,占调查机构的 75 65 %。从事防保工作的人数为 1 1 36人 ,平均每个防保科为 2人。大同市和太原市防保科配备的防保人员相对较多 ,分别平均为 3 2人和2 6人 ,晋中市和运城市较低 …     

黄芪注射液对缺血性心肌病终末期心衰短期疗效的研究

目的:观察黄芪注射液治疗缺血性心肌病终末期心衰的短期疗效。方法:选取本院2012年6月—2014年3月间收治的缺血性心肌病所致的终末期心衰患者共106例,将其随机分为治疗组和对照组,每组各53例,两组患者均接受规范的冠心病二级预防措施和心力衰竭标准化治疗,治疗组在上述治疗的基础上加用黄芪注射液20 m L+5%葡萄糖注射液250 m L,1次/d,静点,14天为1个疗程。两组患者治疗前后分别通过NYHA心功能分级、6 min步行试验、B型钠尿肽、左室射血分数(LVEF)评估患者心衰程度,同时在治疗前及治疗结束时查血、尿常规,电解质,肝、肾功能、心电图。结果:两组患者治疗前上述指标无统计学意义(P>0.05)。治疗后两组患者心衰程度差异有统计学意义(P<0.05),治疗组NYHA分级、6 min步行试验、B型钠尿肽、左室射血分数(LVEF)均有明显改善,与对照组比较均有统计学意义(P<0.05)。治疗前后患者血、尿常规,电解质、肝肾功能、凝血系列无明显异常。结论:黄芪注射液可以安全有效的改善缺血性心肌病终末期心衰程度。     

国产凝血酶治疗肝硬化上消化道出血止血疗效的初步观察(摘要)

我们用纤维胃镜对16例肝炎后肝硬化、上消化道出血患者进行检查,并用凝血酶局部止血治疗,效果满意。本组男12例,女4例,年龄24～62岁,于上消化道出血24～48小时内作胃镜检查,诊为食道静脉曲张出血7例(43.8％),食道静脉曲张并牯膜糜烂出血     

三维容积超声检测胎儿胸腺对早期诊断妊娠高血压的价值

目的 探讨三维容积超声检测胎儿胸腺对早期诊断妊娠高血压的价值.方法 选取2018年6月至2019年11月本院收治的60例妊娠期高血压产妇作为观察组,选取同期于本院体检的孕周匹配的60名健康产妇作为对照组,所有产妇均予以二维超声检查与三维容积超声检查胎儿胸腺,并测量胎儿胸腺前后径(APD)、胸腺横径(MTD)、胸腺-胸廓比(T-T值),采用虚拟器官计算机辅助分析(VOCAL)技术计算出胎儿胸腺体积,比较两组APD、MTD、T-T值及胸腺体积.结果 观察组APD、MTD、T-T值及胸腺体积均大于对照组,差异有统计学意义(P<0.05).结论 通过三维容积超声检测胎儿APD、MTD、T-T比值及采用VOCAL技术计算胸腺体积对于早期诊断妊娠高血压具有较高临床价值,值得临床推广应用.     

小胶质细胞极化在脊髓损伤中的作用与机制研究进展

脊髓损伤(spinal cord injury,SCI)是一种常见的难治性疾病,一直是临床研究热点.它不仅损害患者的身心健康,也给家庭及社会带来沉重的经济负担.虽然对SCI的研究有很多,然而迄今为止对SCI的确切机制仍不清楚,因此缺乏有效的治疗手段.近年的相关研究表明,小胶质细胞极化后引起的炎症反应在SCI中扮演重要角色.本文就小胶质细胞极化在SCI中的作用与机制进行综述,希望能为SCI治疗提供新的策略.     

山西省传染性非典型肺炎早期预警监测试点监测工作分析

传染性非典型肺炎(SARS)作为一种危害严重的新发传染病,人类对其传染来源、流行特征及发病机理还未能充分认识,也没有证据表明任何地区任何人群出现针对SARS的免疫屏障,已经感染发病人群的免疫持久性尚待持续观察;SARS作为一种呼吸道传染病是否存在常见的冬春季节高峰?这些因素都可能导致SARS重来。因此,根据中国疾病预防控制中心要求,山西省选择了两所医院从2003年12月10日至2004年1月11日进行了为     

Ras相关的C3肉毒毒素底物1的小发夹RNA干扰活性氧-核因子κB通路抑制小鼠视网膜新生血管生成

Objective To investigate the effects of knocking down Racl gene (ras-related C3 botulinum toxin substrate 1) by small hairpin RNA (shRNA) on retinal neovascularization in a mouse model of oxygen-induced retinopathy (OIR). Methods One hundred and eight 7-day-old C57BL/6J mice were divided into three groups randomly. The OIR was induced by Smith protocol in 2 groups. OIR mice received an intravitreal injection of Racl-shRNA plasmid or the nonsense plasmid in the gene-intervention group and control group respectively at the age of postnatal day 11 (P11). Non-OIR mice also received an intravitreal injection of Racl-shRNA plasmid at P11 as the blank-intervention group which lived in the normoxic environment. Retinal neovascularization was investigated on flat-mounts after fluorescence angiography at P15 and P17. Endothelial cell nuclei breaking through the internal limiting membrane were counted on pathological section at P17. The expression of Racl and NF-κB p65 subunit was measured by immuohistochemistry, Western blot, real-time polymerase chain reaction (RT-PCR) and in situ hybridization. Results Compared with the blank-control group, the level of Racl mRNA in the geneintervention group decreased obviously(t=4.5, P = 0. 001 ); the retinal non-perfusion areas, fluorescence leakage, neovascularization and the number of endothelial cell nuclei breaking through the internal limiting membrane were reduced significantly(t = 6. 521, P< 0. 001) ; the level of NF-κB p65 nuclear translocation decreased(t= 16. 008, P<0. 001)while the expression of NF-κB p65 mRNA was reduced obviously(t=3. 354, P=0. 006), which was positively correlated with the expression of Ratl mRNA (P=0. 012).Conclusion Intravitreal injection of Racl-shRNA with liposome in mice can effectively inhibit the expression of Racl, and inhibit the retinal neovascularization under relative hypoxia via blocking the ROS-NF-κB pathway.     

不同产地水线草中多糖含量比较

目的：测定不同产地水线草中多糖含量。方法：采用紫外分光光度法测定水线草中多糖含量。结果：田间伴生水线草多糖平均含量为2.20%,野生水线草多糖平均含量为1.93%。结论：水线草由野生转化为家种,有利于保证药材的有效性和稳定性。     

Ras相关的C3肉毒毒素底物1的小发夹RNA干扰活性氧-核因子κB通路抑制小鼠视网膜新生血管生成

Objective To investigate the effects of knocking down Racl gene (ras-related C3 botulinum toxin substrate 1) by small hairpin RNA (shRNA) on retinal neovascularization in a mouse model of oxygen-induced retinopathy (OIR). Methods One hundred and eight 7-day-old C57BL/6J mice were divided into three groups randomly. The OIR was induced by Smith protocol in 2 groups. OIR mice received an intravitreal injection of Racl-shRNA plasmid or the nonsense plasmid in the gene-intervention group and control group respectively at the age of postnatal day 11 (P11). Non-OIR mice also received an intravitreal injection of Racl-shRNA plasmid at P11 as the blank-intervention group which lived in the normoxic environment. Retinal neovascularization was investigated on flat-mounts after fluorescence angiography at P15 and P17. Endothelial cell nuclei breaking through the internal limiting membrane were counted on pathological section at P17. The expression of Racl and NF-κB p65 subunit was measured by immuohistochemistry, Western blot, real-time polymerase chain reaction (RT-PCR) and in situ hybridization. Results Compared with the blank-control group, the level of Racl mRNA in the geneintervention group decreased obviously(t=4.5, P = 0. 001 ); the retinal non-perfusion areas, fluorescence leakage, neovascularization and the number of endothelial cell nuclei breaking through the internal limiting membrane were reduced significantly(t = 6. 521, P< 0. 001) ; the level of NF-κB p65 nuclear translocation decreased(t= 16. 008, P<0. 001)while the expression of NF-κB p65 mRNA was reduced obviously(t=3. 354, P=0. 006), which was positively correlated with the expression of Ratl mRNA (P=0. 012).Conclusion Intravitreal injection of Racl-shRNA with liposome in mice can effectively inhibit the expression of Racl, and inhibit the retinal neovascularization under relative hypoxia via blocking the ROS-NF-κB pathway.     

经角膜缘隧道切口硅油取出的临床观察

目的 探讨对部分玻璃体切除联合硅油填充眼经角膜缘隧道切口取出眼内硅油的临床应用及优缺点.方法 硅油填充眼42眼(无晶体34眼、伴白内障8眼).灌注针头自颞下方角膜缘隧道切口插入前房,行灌注;连接了负压吸引管的20G静脉穿刺套管经12点位角膜缘隧道切口插入前房,抽吸硅油.白内障眼先行晶体超声乳化吸出及后囊切开,无晶体眼后囊完整者亦先行后囊切开.伴白内障者有3眼行一期人工晶体植入,无晶体者有6眼行二期人工晶体植入.记录取硅油时间,观察术后眼部炎症反应、角膜水肿、视力、眼压等,出院后随访4～12周[平均(7.45±2.67)周].结果 42眼均一次性取净硅油,取出时间15～45 min[平均(27.67±10.41) min],术后眼压5.8～18.2 mmHg [平均(14.61±3.47) mmHg,1 mmHg=0.133 kPa],视力较术前稳定或提高,术后并发症主要为局部暂时性的轻微炎症反应:结膜充血(90.48%)、角膜轻度水肿(90.48%)、房水混浊(19.05%),均2～4 d消退;一过性低眼压有11.9%,5～7 d恢复正常;视网膜再脱离者有7.14%.结论 经角膜缘隧道切口的硅油取出术,简便省时、安全有效且并发症少,适用于无晶状体及需同时摘除白内障的硅油填充眼.