非小细胞肺癌循环肿瘤细胞的定量检测意义

Objective To investigate the sensitivity, specificity and clinical significance of detecting circulating tomor cells (CTCs) in NSCLC. Methods Twenty-five patients who undetwent surgical resection for NSCLC form Jan 2007 to Apr 2007 were in- cluded in this study. Control group included 10 patients with benign pulmonary diseses (2 case of hamartoma and 8 case of pulmonary tu berculosis) and 10 healthy volunteers. The pulmonary veins blood and peripheral vein blood were collected respectively. The CD326 immunomagnetic beads and CK-fluorescein isothiocyanate (CK-FITC) were served as the marker antibodies of CTCs. Firstly mononu- clear cell marked by minibeads conjugated with CD326, the mononuclear cells were enriched and separated though Magnetic-activated cell separation(MACS), then the positive separtion cells were marked by anti-CK-FTTC and anti-leukocyte antibody CD45-phyco- erythrine (anti-CD45-PE), finally those cells detected and analyzed by flow cytometry . Results For stage Ⅰ and stage Ⅱ patients (n= 16), CTCs were detected from peripheral vein blood of in 5 (5/16, 31.25%) and from pulmonary veins blood in 9 (9/16, 56.25%). For stage Ⅲ and stage Ⅳ, CTCs were detected from peripheral vein blood in 5 (5/9, 55.56%) and from pulmonary veins blood in 9(7/9,77.78%).For stage Ⅰ,CTCs were detected from peripheral vein blood in 3(3/13,23%)and from pulmo- nery veins blood in 8(8/13,61.54%).The whole CTCs positive detection rate from pilmonary veins blood was 64%(16/25)which higher than feom peripheral vein blood(40%,10/25)(P<0.05).Conclusion The method was set up by MACS combined with FCM to detect the CTCs of pulmonary veins blood and peripheral vein blood of patients.with NSCLC,MACS combined with FCM may improve detection rate of CTCs.This technique appears to be an efficient to detect circulating tumor cells and may be important for dinical practice in the future.     

应用葡萄糖利尿作用对输尿管结石彩色多普勒超声诊断的价值

输尿管结石是泌尿外科常见疾病 ,诊断主要依赖Ｘ线检查 ,但如结石阴影与骨骼重叠或Ｘ线不显影的结石 ,则诊断极为困难。超声检查则不受上述因素的影响 ,且操作方便、无创、无痛苦。通过口服5 0 %的葡萄糖溶液 2 0ｍｌ,并饮水 3 0 0～ 5 0 0ｍｌ,能排除肠腔气体干扰 ,改变声阻抗 ,使输尿管扩张 ,在尿液的衬托之下 ,可清晰显示输尿管结石的声像图 ,具有一定临床应用价值 ,报告如下。1　资料与方法1 1　研究对象　本组 5 2 0例均为门诊及住院病例 ,男性 42 6例 ,女性 94例 ,年龄 2 1～ 61岁 ,平均 45 3岁。1 2　使用仪器　Ａｌｏｋａ 1 70 0…     

略论《金匮·虚劳病》篇的扶正固本法则

虚劳病乃因劳致损,积损成虚,久虚不复的一类慢性虚衰性疾患的总称,它概括了临床所常见的多种虚损病证。病至虚劳,人体的脏腑气血阴阳表现某方面或多方面的严重亏损,所以在治疗中更应突出强调扶正固本治则。仲景的《虚劳病》篇所述治法,正是这一法则的综合应用及集中体现,颇为后世医家推崇和效法,试从以下两方面略加阐述。     

非小细胞肺癌循环肿瘤细胞的定量检测意义

Objective To investigate the sensitivity, specificity and clinical significance of detecting circulating tomor cells (CTCs) in NSCLC. Methods Twenty-five patients who undetwent surgical resection for NSCLC form Jan 2007 to Apr 2007 were in- cluded in this study. Control group included 10 patients with benign pulmonary diseses (2 case of hamartoma and 8 case of pulmonary tu berculosis) and 10 healthy volunteers. The pulmonary veins blood and peripheral vein blood were collected respectively. The CD326 immunomagnetic beads and CK-fluorescein isothiocyanate (CK-FITC) were served as the marker antibodies of CTCs. Firstly mononu- clear cell marked by minibeads conjugated with CD326, the mononuclear cells were enriched and separated though Magnetic-activated cell separation(MACS), then the positive separtion cells were marked by anti-CK-FTTC and anti-leukocyte antibody CD45-phyco- erythrine (anti-CD45-PE), finally those cells detected and analyzed by flow cytometry . Results For stage Ⅰ and stage Ⅱ patients (n= 16), CTCs were detected from peripheral vein blood of in 5 (5/16, 31.25%) and from pulmonary veins blood in 9 (9/16, 56.25%). For stage Ⅲ and stage Ⅳ, CTCs were detected from peripheral vein blood in 5 (5/9, 55.56%) and from pulmonary veins blood in 9(7/9,77.78%).For stage Ⅰ,CTCs were detected from peripheral vein blood in 3(3/13,23%)and from pulmo- nery veins blood in 8(8/13,61.54%).The whole CTCs positive detection rate from pilmonary veins blood was 64%(16/25)which higher than feom peripheral vein blood(40%,10/25)(P<0.05).Conclusion The method was set up by MACS combined with FCM to detect the CTCs of pulmonary veins blood and peripheral vein blood of patients.with NSCLC,MACS combined with FCM may improve detection rate of CTCs.This technique appears to be an efficient to detect circulating tumor cells and may be important for dinical practice in the future.     

非小细胞肺癌循环肿瘤细胞的定量检测意义

Objective To investigate the sensitivity, specificity and clinical significance of detecting circulating tomor cells (CTCs) in NSCLC. Methods Twenty-five patients who undetwent surgical resection for NSCLC form Jan 2007 to Apr 2007 were in- cluded in this study. Control group included 10 patients with benign pulmonary diseses (2 case of hamartoma and 8 case of pulmonary tu berculosis) and 10 healthy volunteers. The pulmonary veins blood and peripheral vein blood were collected respectively. The CD326 immunomagnetic beads and CK-fluorescein isothiocyanate (CK-FITC) were served as the marker antibodies of CTCs. Firstly mononu- clear cell marked by minibeads conjugated with CD326, the mononuclear cells were enriched and separated though Magnetic-activated cell separation(MACS), then the positive separtion cells were marked by anti-CK-FTTC and anti-leukocyte antibody CD45-phyco- erythrine (anti-CD45-PE), finally those cells detected and analyzed by flow cytometry . Results For stage Ⅰ and stage Ⅱ patients (n= 16), CTCs were detected from peripheral vein blood of in 5 (5/16, 31.25%) and from pulmonary veins blood in 9 (9/16, 56.25%). For stage Ⅲ and stage Ⅳ, CTCs were detected from peripheral vein blood in 5 (5/9, 55.56%) and from pulmonary veins blood in 9(7/9,77.78%).For stage Ⅰ,CTCs were detected from peripheral vein blood in 3(3/13,23%)and from pulmo- nery veins blood in 8(8/13,61.54%).The whole CTCs positive detection rate from pilmonary veins blood was 64%(16/25)which higher than feom peripheral vein blood(40%,10/25)(P<0.05).Conclusion The method was set up by MACS combined with FCM to detect the CTCs of pulmonary veins blood and peripheral vein blood of patients.with NSCLC,MACS combined with FCM may improve detection rate of CTCs.This technique appears to be an efficient to detect circulating tumor cells and may be important for dinical practice in the future.     

抗白灵散的质量标准研究

目的:建立抗白灵散的质量标准。方法:采用显微鉴别法对蜈蚣进行鉴别;采用TLC法对丹参、青黛进行定性鉴别;采用滴定法测定雄黄中二硫化二砷的含量。结论:方法结果准确,重现性好,可用于抗白灵散的质量控制。     

中医针刺及按摩治疗腰麻术后尿潴留32例报告

在临床工作中 ,尿潴留是腰麻术后常见的并发症 ,如果及时采取有效的方法 ,解除尿潴留 ,可为患者解除痛苦。临床上常用留置导尿方法 ,它的弊病是增加了尿路逆行感染 ,给患者带来了痛苦 ,自 1 996年以来 ,我们采用中医针刺及按摩治疗腰麻术后尿潴留 ,收到明显效果 ,现报告如下。资料与方法1 .1 .一般资料中医治疗组 3 2例 ,男 ,2 0例 ,女 ,1 2例 ;年龄 9～ 50岁 ,平均年龄 3 0岁 ,其中年龄 9～ 1 1岁 5例 ,1 2～ 1 9岁1 0例 ,2 0岁以上 1 7例。西医治疗组 2 0例 ,男 ,1 1例 ,女 ,9例 ;年龄 9～ 50岁 ,平均 2 8岁 ,其是年龄在 9～ 1 1岁 2例 ,1…     

甲状腺嗜酸性细胞肿瘤8例

甲状腺嗜酸性细胞肿瘤[Hurthle cell（oncocytic）tumor]是指包括那些完全或主要（超过75％）由具有嗜酸性细胞特征的滤泡细胞构成的肿瘤。临床很少见,目前有关这种疾病的诊断标准与治疗方法文献报道很少,且对该病的认识尚未统一。而研究该病的病理特点和生物学特性,对诊断及其预后有十分重要的意义。笔者于1996～2003年收治8例该病患者,现报告如下。     

睾丸网囊性发育异常1例

患者 ,男 ,64岁。以左阴囊内肿物 8年入院。 8年前发现左侧阴囊内肿物 ,约鸡蛋大小 ,无不适症状 ,未诊治。 8年来肿物逐渐增大 ,入院前 3个月肿物增大加快 ,约鹅蛋大小 ,伴下坠感。体检 :一般状况良好 ,心肺无异常 ,腹平软 ,肝、胆、脾、双肾未触及 ,无压痛 ,肝、肾区无叩痛 ,阴茎发育正常 ,左侧阴囊内触及 8.0 cm× 7.0 cm× 6.0 cm肿物 ,无触痛 ,光滑 ,囊性感 ,睾丸及附睾未触及 ,肿物与阴囊皮肤无粘连 ,透光试验阳性 ,不能还纳入腹腔 ,右侧睾丸及附睾正常。彩色超声示左侧睾丸显著增大 ,中心部见一个巨大囊性无回声暗区 ,将睾丸组织挤压…