14-脱羟-11，12-二脱氢穿心莲内酯对糖尿病大鼠血糖及肿瘤坏死因子-α的影响

目的观察14-脱羟-11,12-二脱氢穿心莲内酯对链脲佐菌素（streptozotocin,STZ）所致的糖尿病（diabetesmellitus,DM）大鼠血糖及肿瘤坏死因子-α（TNF-α）的影响。方法采用腹腔注射STZ建立DM模型,将成模大鼠随机分为模型组、低剂量组、高剂量组,并设正常对照组。给药14d后,断尾采血测血糖,苏木精和伊红（HE）染色观察胰岛的形态,免疫组织化学检测TNF-α的表达。结果与正常组比较,模型组血糖明显升高（P〈0．05）,胰腺组织病理损害严重,TNF-α表达呈强阳性;与模型组比较,低、高剂量组血糖均下降（P〈0．05）．胰腺组织病理损害减轻,TNF-α表达下调。结论糖尿病时胰岛β细胞中的TNF-α表达增高,14-脱羟-11．12-二脱氢穿心莲内酯可降低血糖,下调TNF-α的表达,减轻胰腺的病理损伤,改善胰岛功能。     

Downregulation of p38 MAPK involved in inhibition of LDL-induced proliferation of mesangial cells and matrix by curcumin

Curcumin, as a main pharmacological component in the traditional Chinese medicine-- tttrmeric, has shown anti-inflammatory, anti-oxidation, anti-tumor and anti-fibrotic effects. This study aimed to investigate the possible underlying signaling pathway which was involved in the inhibition of LDL-induced proliferation of mesangial cells and matrix by curcumin. Rat mesangial cells in vitro were incubated with low-density lipoprotein （LDL） and different concentrations of curcumin （0, 6.25, 12.5, 25.0 9mol/L） or p38 MAPK inhibitor, SB203580 （10 μmol/L）. Under LDL incubation, mesangial cells proliferated, the expression of MMP-2 mRNA and protein was decreased, the expression of COX-2 mRNA and protein was increased, reactive oxygen species （ROS） generation was increased and p38 MAPK was activated significantly （P〈0.05）. When LDL-induced cells were treated with curcumin in the concentration of 12.5 or 25.0 μmol/L, LDL-induced proliferation ofmesangial cells was suppressed, the expression of MMP-2 mRNA and protein increased, the expression of COX-2 mRNA and protein downregulated, the production of ROS inhibited and p38 MAPK inactivated （P〈0.05）. In conclusion, curcumin can inhibit the LDL-induced proliferation of mesangial cells and up-regulate the expression of MMP-2, which may be related with the inhibitory effect of curcumin on COX-2 expression, ROS pro- duction and p38 MAPK.     

Erbin interacts with Sema4C and inhibits Sema4C-induced epithelial-mesenchymal transition in HK2 cells

Erbin, a member of Leucine-rich repeat and PDZ-containing protein family, was found to inhibit TGF-β-induced epithelial-mesenchymal transition （EMT） in our previous study. However, the mechanism of Erbin in regulating EMT is unclear. Semaphorin protein Sema4C, with PDZ binding site at C-terminal has been recognized as a positive regulator of EMT. Here, we aimed to examine the inter- action between Erbin and Sema4C. HK2 cells were treated with TGF-β1, or transfected with Erbin and （or） Sema4C. Interaction of Erbin and Sema4C was identified by immunoprecipitation. RT-PCR was used to detect the expression of Erbin and Sema4C at mRNA level after transfection. The expression levels of Erbin, Sema4C, and markers of EMT were measured by using Western blotting or ELISA. Af- ter HK2 cells were stimulated with 10 ng/mL TGF-β1 for 72 h, the protein expression levels of Erbin and Sema4C were both up-regulated, and immunoprecipitation results showed Erbin interacted with Sema4C in HK2 cells both at endogenous and exogenous levels. Furthermore, overexpression of Sema4C suppressed E-cadherin, induced vimentin and promoted fibronectin secretion, indicating Sema4C promotes the process of EMT. However, HK2 cells overexpressing Erbin were resistant to Sema4C-induced EMT. In contrast, Erbin specific siRNA promoted EMT induced by Sema4C. Taken together, these results suggest that Erbin can interact with Sema4C, and co-expression of Erbin blocks the process of Sema4C-induced EMT.     

脑血管病患者不同心理状态的护理

本文对125例脑血管病患者在接受治疗时,作了全面调查,结果发现患者有不同程度的心理障碍,特别是职业对患者亦有显著影响。我们针对以上不同心理状态的患者,实施有效的心身护理,取得了满意的效果。     

重组人内皮抑素结合化疗对晚期非小细胞肺癌患者血清LMTK-3、IGFBP-7 的影响

目的 探讨重组人内皮抑素结合化疗对晚期非小细胞肺癌患者血清Lemur 酪氨酸激酶3（LMTK-3）、胰岛素样生长因子结合蛋白7（IGFBP-7）的影响。方法 选取2012 年1 月-2015 年1 月于该院收治的NSCLC 患者86 例为研究对象,按随机数字表法分为观察组和对照组,每组43 例。对照组患者采取吉西他滨+顺铂化疗。观察组在对照组基础上联合恩度治疗。对两组患者的临床疗效、不良反应、外周血血管内皮生长因子（VEGF）、胰岛素样生长因子1（IGF-1）、LMTK-3、IGFBP 表达水平进行评价。结果 观察组治疗客观有效率、总有效率高于对照组（P <0.05）,观察组的疗效优于对照组,差异有统计学意义（P <0.05）。两组患者的不良反应发生率并差异无统计学意义（P >0.05）。两组患者治疗后VEGF、IGF-1 及LMTK-3 水平较治疗前更低,GFBP 水平较治疗后更高,差异有统计学意义（P <0.05）,且观察组治疗后VEGF、IGF-1 及LMTK-3 水平较对照组更低,IGFBP-7 更高,差异有统计学意义（P <0.05）。结论 重组人内皮抑素联合化疗可有效降低NSCLC 患者的血清LMTK3 水平,升高血清IGFBO-7 水平,患者的疗效良好,不良反应较低。     

左氧氟沙星不良反应13例报告

左氧氟沙星是在临床上应用较广的广谱抗菌素,近年来随着使用的增加,过敏反应的出现越来越多,本文作者对吉林大学南岭校区医院使用左氧氟沙星出现过敏反应的13例患者临床资料进行分析,报告如下. 1 临床资料 本组患者13例为本院近5年来住院及门诊病历,男性8例,女性5例;年龄18～71岁(18岁以下禁用),其中18～30岁5例,31～50岁4例,51～71岁4例;有其他药物过敏史4例,除药物以外其他因素过敏史2例.左氧氟沙星给药途径为静脉滴注400 mg (200mL),滴速3 mg·min-1.滴注后5～30 min后出现过敏反应9例,30～60 min内过敏反应4例.临床表现为皮肤瘙痒9例,荨麻疹7例,口腔黏膜及皮肤水泡3例,过敏性休克1例,两种以上临床表现7例,过敏后临床处理:出现症状后立即拔掉液体,轻症病例给抗组胺药物扑尔敏等口服;维生素C2g,10％葡萄糖酸钙20 mL加入5％葡萄糖250mL静脉滴注,1次/日;局部外搽含有樟脑或薄荷的炉甘石洗剂,以止痒、散热、消炎,病情稍重的休克患者积极抗休克治疗,及早给予糖皮质激素.经治疗,轻度皮疹1周内治愈,严重皮疹2周内治愈.无死亡病例.     

单核细胞趋化蛋白-1对系膜细胞增殖及纤维连接蛋白和Ⅰ型胶原基因表达的影响

目的 观察单核细胞趋化蛋白-1（MCP-1）对体外培养的大鼠系膜细胞增殖及纤维连接蛋白（FN）和Ⅰ型胶原（COLI）基因表达的影响。方法 采用不同浓度的MCP-1刺激体外培养的系膜细胞,于不同的时间应用MTT法测定系膜细胞增殖活性,半定量RT-PCR的方法测定细胞FN和COLⅠ mRNA的相对表达量。结果 随着MCP-1浓度的增高,刺激时间的延长,MCP-1明显促进系膜细胞的增殖、上调细胞FN和COLⅠ mRNA的表达（P〈0．01）,表现为剂量和时间依赖性。结论 MCP-1能促进大鼠系膜细胞的增殖及上调细胞FN和COLⅠ mRNA的表达。MCP1可能在系膜增生性。肾小球肾炎的发病过程中起一定的作用。     

利凡诺配伍米非司酮终止中期妊娠疗效观察

近年来利凡诺羊膜腔注入引产在基层医院仍是首选，自2000年-2003年我院采用利凡诺配伍米非司酮终止中期妊娠与单用利凡诺引产比较，效果良好，现报道如下：     

79例新生儿缺氧缺血性脑病的护理体会

新生儿缺氧缺血性脑病(HIE)在新生儿科颇为常见,临床上伴有不同程度的神经系统症状,无特效的治疗药物,加强护理是降低死亡率及减少后遗症发生率的重要措施。1998年4月-1999年7月我院新生儿科共收治HIE79例,现将护理体会总结如下。1临床资料