激光闭合术治疗下肢静脉曲张患者的护理

下肢静脉曲张是血管外科最常见的疾病。传统大隐静脉曲张高位结扎及剥脱术治疗，由于手术创伤大、术后瘢痕明显、康复时间长等原因，患者往往难以接受。自2006年10月以来，我科对18例患者采用激光闭合术治疗下肢静脉曲张，取得了满意的疗效．现报道如下。[第一段]     

Differentiation of human epidermal stem cells into fibroblasts induced by TGF-β1 in vitro

Objective To investigate the correlation between human epidermal stem cell (hESCs) and hypertrophic scar or keloid. Methods Improved collagen Ⅳ-coated adhesion methods was used to isolate and culture the epidermal stem cells after neutral protease selectively digested the dermo-epidermal junctions. After the cells were cultured and expanded in vitro, and passage 3 hESCs were induced by different concentrations of TGF-β1 (0.1, 5.0, and 10.0 ng/ml). Morphological fea-tures and identification of these cells were meseasured by HE, Masson, immunohistochemical staining on the days 3 and 7, respectively. Results After induced by TGF-β1 for 3 and 7 days, the morpholo-gy of the epidermal stem cell (hESCs) was changed into fusiform shape, similar to fibroblasts. 70 % ofthe cell which was induced by TGF-β1 were blue stained in the cytoplasm by Masson stain, which is the distinctive method for collagen, suggesting collagen appeared or increased in the cells. The collagen concentrations in supernatants of hESCs were 0.4150±0.0014, 0.3380±0. 0020, and 0.3870±0.0020, much higher than that in control group (0.0780±0.0025) and normal skin fibro-blast group (0.15004±0.0051) (P<0.05). Immunohistochemical staining revealed that positive rates of these cells for anti-vimentin staining were more than (95.00±1.20)% in experiments and (5.70±0.20)% in control group. Conclusion The differentiantion of hESCs induced by TGF-β1 into fibro-blasts indicates that hESCs may play a role in the pathogenesis of hypetrophic scar and keloid.     

玉溪市首次发现艾滋病并弓形虫脑病1例

病例患者，男性，41岁，已婚，农民，因“间断发热、消瘦2月，伴发应迟钝、尿失禁3d”入院。患者既往有性乱史，4年前患过带状疱疹。患者家中饲养有狗。患者近2个月来无明显诱因出现间断发热，以午后发热为主，体温波动在37℃～38.0℃之间，无咳嗽、盗汗、腹痛、腹泻，近2个月体重无诱因下降10kg，无手抖、脾气暴躁，多次到我院门诊就诊，拟诊“上呼吸道感染”予抗生素治疗后，仍时有低热，近3d他人发现其反应迟钝，少言懒语，小便失禁，精神、饮食差，以“发热待查：①结核？②沙门氏菌感染？③AIDS？”收住院。     

二巯基丁二酸胶囊现场诊治铅中毒37例分析

我们应用二琉基丁二酸胶囊(DMSA)对37例铅中毒患者进行治疗．疗效较好，现报告如下。     

临床路径在经输尿管镜治疗输尿管结石病人中的应用研究

[目的]探讨临床路径在经输尿管镜治疗输尿管结石病人中的应用效果。[方法]将44例实施临床路径后病人的平均住院天数、平均住院费用、病人对健康知识掌握情况及病人满意度与44例实施临床路径前的病人进行比较。[结果]病人住院天数由6.17d±0.36d下降至5.18d±0.22d,住院费用由5104.0元±126.0元下降至4619.0元±101.0元,病人健康知识掌握情况由25.4分±2.20分增加至27.3分±1.70分,病人满意度由90.2%上升至94.4%。[结论]按照临床路径对病人实施医疗护理照顾,有利于提高护理质量,提高病人满意度,提升医院的外部形象。     

骨巨细胞瘤的放射治疗

１９７０年至１９９３年共放射治疗骨巨细胞瘤２２例。单纯放疗３例，术后放疗１４例，术后复发放疗５例。肿瘤剂量＜４８Ｇｙ６例，４０～５０Ｇｙ１３例，＞５０Ｇｙ３例。全组５年生存率９５．％（２０／２１），１０年生存率８７．５％的（１４／１６）。２例分别于放疗后半年及７年死于肿瘤肺转移。２０例至今健在，最长者已存活２６年，均能正常生活及工作，未发现恶变成照射区内第二恶性肿瘤发生。我们认为对不能手术、手术不彻底、术后复发及病理分级商的骨巨细胞瘤思考。放射治疗为一种有效的治疗手段。     

原子吸收光谱法测定锌制剂的含量

建立了不同剂型锌制剂含量的原子吸收火焰光谱法。该方法简便，快速，各种剂型只需简单处理后即可直接测定。洗剂，搽剂及散剂回收纺为９９．１％，软膏剂，糊剂回收率为９８．０％，滴眼剂回收率为１０１．６％，片剂回收率为１００．３％。该方法尤其适用于制剂半成品的快速检验。     

血清基质金属蛋白酶与糖尿病血管病变的关系

目的 观察卡维地洛治疗慢性心力衰竭(CHF)的疗效并初步探讨卡维地洛防治心力衰竭的机理。方法 观察卡维地洛治疗34例CHF患者的疗效和对氧自由基、血液流变学的影响，并与用常规治疗(利尿剂、洋地黄、ACEI)的29例作对照。结果 卡维地洛组较对照组LVEF、FS显著升高，LVDD、LVSD显著下降，能明显升高血清超氧化物歧化酶活性(SOD)活性，降低血清丙二醛(MAD)水平，降低全血黏度和血浆黏