Differentiation of human epidermal stem cells into fibroblasts induced by TGF-β1 in vitro

Objective To investigate the correlation between human epidermal stem cell (hESCs) and hypertrophic scar or keloid. Methods Improved collagen Ⅳ-coated adhesion methods was used to isolate and culture the epidermal stem cells after neutral protease selectively digested the dermo-epidermal junctions. After the cells were cultured and expanded in vitro, and passage 3 hESCs were induced by different concentrations of TGF-β1 (0.1, 5.0, and 10.0 ng/ml). Morphological fea-tures and identification of these cells were meseasured by HE, Masson, immunohistochemical staining on the days 3 and 7, respectively. Results After induced by TGF-β1 for 3 and 7 days, the morpholo-gy of the epidermal stem cell (hESCs) was changed into fusiform shape, similar to fibroblasts. 70 % ofthe cell which was induced by TGF-β1 were blue stained in the cytoplasm by Masson stain, which is the distinctive method for collagen, suggesting collagen appeared or increased in the cells. The collagen concentrations in supernatants of hESCs were 0.4150±0.0014, 0.3380±0. 0020, and 0.3870±0.0020, much higher than that in control group (0.0780±0.0025) and normal skin fibro-blast group (0.15004±0.0051) (P<0.05). Immunohistochemical staining revealed that positive rates of these cells for anti-vimentin staining were more than (95.00±1.20)% in experiments and (5.70±0.20)% in control group. Conclusion The differentiantion of hESCs induced by TGF-β1 into fibro-blasts indicates that hESCs may play a role in the pathogenesis of hypetrophic scar and keloid.     

干槽症的细菌学研究及甲硝唑微栓预防和治疗应用

本文通过对19例拔牙后干槽症患者牙窝内容物的细菌培养和药敏试验,对引起该病的特殊细菌和病因进行了探讨。同时采用甲硝唑微栓置入383例下颌阻生第三磨牙拔牙创内,观察其预防和治疗干槽症的效果,结果表明甲硝唑微栓在预防和治疗干槽症上具有明显效果。     

78结合牙1例

患者张某，女，52岁，因左上后牙自发痛2月余来诊。此牙2年前曾诊治。2月前此牙痛复发，在当地医院诊治至今，目前仍有咬合痛，偶有自发痛，来我科要求拔除，检查：7倾侧远中处银汞充填，近中洞达根底，叩（ ），松动（-），牙龈（-），8未查及，无牙列拥挤，根尖片：7根尖透射影，8阻生，78相连，其间未见牙周膜，诊断：（1）7慢性根尖周炎；（2）78为结合牙（8阻生）。     

湘西土家族、苗族、侗族癫痫流行病学调查

湘西土家族、苗族、侗族癫痫流行病学调查王华，雷花香，黄定梅，邓朝晖多年来，虽然癫痫的流行病学研究较多，但有关土家族、苗族、侗族的癫痫调查资料却极少。作者于１９９２年３月～７月对居住在湘西的这三个民族的人群进行了癫痫的流行病学调查。现将有关资料报告如下...     

缺血性心脏病(CHD)30例临床治疗

1998－01～1998－07我们应用生脉注射液治疗CHD30例，取得较好疗效，现将结果报道如下。l资料与方法1．l病例选择。本组60例，均有不同程度的胸闷、心悸、气短症状；心电（ECG）示心肌缺血性改变（ST－T改变）；二维心动超声（＊＊G）示左小室舒张功能降低，随机分成两组。治疗组，男16例，女14例，平均年龄64岁；对照组，男17例，女13例，平均年龄63岁。l．2方法。治疗组：常规疗法加生脉注射液6Oml溶于5％Glmoze或O．85％Natriichloricli25Oml中ivgtt．qd，两周一疗程。对照组：常规疗法，硝酸酯类药物，和／或加用p受体阻滞剂．和…     

高剂量率60Coγ射线照射离体人血建立染色体畸变的剂量-效应曲线

目的　高剂量率 6 0Coγ射线照射离体人血建立染色体畸变的剂量 -效应曲线。方法　6 0 Coγ射线照射离体人血 ,吸收剂量为 1 0～ 8 0Gy ,剂量率为 3 0Gy/min ,染色体采用微量法 ,培养开始加秋水仙碱 ,主要记录染色体型畸变的非稳定性畸变 ,包括无着丝粒断片、微小体、无着丝粒环、双着丝粒体和着丝粒环 (双 +环 ) ,对实验数据做曲线拟合 ,并检验回归系数的显著性和曲线的拟合度。结果　6 0 Coγ射线照射离体血诱发的“双 +环” ,在 0～8 0Gy范围内 ,呈良好的剂量 -效应关系 ,拟合的最佳回归方程为Y =0 2 69792D + 7 73 0 83× 10 - 2 D2 (R =0 99198)。结论　高剂量率 6 0 Coγ射线照射离体人血 ,采用微量法培养染色体 ,在 0～ 8 0Gy范围内 ,“双 +环”最佳数学模式为Y =bD +cD2 。     

竖横针法结合推拿治疗梨状肌综合征78例

梨状肌综合征是因梨状肌损伤引起的以臀及下肢胀痛，麻木发凉等为主要症状的一组综合征。梨状肌起于第2-4骶骨前面，肌纤维穿坐骨大孔止于股骨大粗隆，为大腿的外旋肌并协助外展后伸，常可因突然旋转或外展大腿及跌扑而损伤，外伤后，治疗不及时而致慢性损伤，因靠近坐骨神经及臀上下神经及动静脉而出现一系列刺激症状，