全文获取类型
收费全文 | 92篇 |
免费 | 5篇 |
国内免费 | 6篇 |
专业分类
儿科学 | 2篇 |
基础医学 | 18篇 |
口腔科学 | 9篇 |
临床医学 | 17篇 |
内科学 | 2篇 |
外科学 | 3篇 |
综合类 | 19篇 |
预防医学 | 25篇 |
药学 | 1篇 |
中国医学 | 7篇 |
出版年
2023年 | 4篇 |
2022年 | 1篇 |
2021年 | 1篇 |
2019年 | 1篇 |
2018年 | 1篇 |
2016年 | 2篇 |
2015年 | 3篇 |
2014年 | 7篇 |
2013年 | 3篇 |
2012年 | 5篇 |
2011年 | 5篇 |
2009年 | 2篇 |
2008年 | 4篇 |
2007年 | 3篇 |
2006年 | 4篇 |
2005年 | 3篇 |
2004年 | 2篇 |
2003年 | 2篇 |
2002年 | 3篇 |
2001年 | 4篇 |
2000年 | 3篇 |
1997年 | 1篇 |
1996年 | 6篇 |
1995年 | 1篇 |
1994年 | 14篇 |
1993年 | 4篇 |
1992年 | 4篇 |
1991年 | 3篇 |
1990年 | 5篇 |
1989年 | 2篇 |
排序方式: 共有103条查询结果,搜索用时 15 毫秒
91.
采用改进的异硫氰酸胍-酚-氯仿一步抽提法提取肠道病毒RNA,通过反复试验,建立了单管一RT一PCR(Reversetranscriptionpolymerasechainreaction)反应体系。结果表明,此法较常规的分步法简便快速、可靠,为临床检测和流行病学研究提供了较理想的方法。 相似文献
92.
1病例报告患者女 ,58岁。因右舌下硬物且反复肿胀1年余于2003年3月17日就诊。患者曾于7年前局麻下行右颌下腺导管结石摘除术 ,术后右颌下腺分泌良好 ,导管通畅。近1年患者自觉右舌下口底处有一硬物且不断增大。反复肿胀 ,尤以在进食酸性食物时肿胀显著 ,一般餐后约10min肿胀可自行消退。时有疼痛 ,影响咀嚼和吞咽。患者曾自行服用抗生素 ,效果不明显。临床检查 :右颌下三角区未触及硬物 ,表面皮肤光滑 ,无充血及肿胀 ,右颌下淋巴结未触及。口腔内检查可见 :右舌下肉阜区红肿 ,右舌下皱襞处肿胀、隆起。双合诊可及条索样感觉并可触及一约2 0c… 相似文献
93.
Objective To study the variation of blood concentration of tetramine in human body with acute tetramine intoxication treated with three different protocols and the levels in human body after discharge from the hos-pital. Methods The blood concentration of tetramine was determined by gas chromatography-mass spectrometry (GC-MS). All 101 patients of acute tetramine intoxication were divided into 3 groups (routine comprehensive treat-ment group,hemoperfusion group,blood transfusion group) according to their blood concentration of tetramine and clinical symptoms. The patients were followed up to monitor the tetramine levels in a year. Results The level of tet-famine in blood was decreased from 33.0(1.7~115.0)μg/L to 18.0(0.3~47.6)μg/L in routine comprehensive treatment group. The total decrement was 45.5 %. The level was decreased from 108.0 (54.0~290.0)μg/L to 26.0 μg/L in hemoperfusion group. The total decrement was 75.9%. The decrement was 20.0%~45.0% after each he-moperfusion. The level was slightly high after 24 h of hemoperfusion. The total decrement in blood transfusion group was 33.5%~60.0%. The level was <0.3μg/L in all 25 out-patients 1 year after their intoxication. Conclusion Routine comprehensive treatment,hemoperfusion,blood transfusion are effective in the treatment of acute tetramine intoxication. The degradation of tetramine in human body is slow. 相似文献
94.
Objective To study the variation of blood concentration of tetramine in human body with acute tetramine intoxication treated with three different protocols and the levels in human body after discharge from the hos-pital. Methods The blood concentration of tetramine was determined by gas chromatography-mass spectrometry (GC-MS). All 101 patients of acute tetramine intoxication were divided into 3 groups (routine comprehensive treat-ment group,hemoperfusion group,blood transfusion group) according to their blood concentration of tetramine and clinical symptoms. The patients were followed up to monitor the tetramine levels in a year. Results The level of tet-famine in blood was decreased from 33.0(1.7~115.0)μg/L to 18.0(0.3~47.6)μg/L in routine comprehensive treatment group. The total decrement was 45.5 %. The level was decreased from 108.0 (54.0~290.0)μg/L to 26.0 μg/L in hemoperfusion group. The total decrement was 75.9%. The decrement was 20.0%~45.0% after each he-moperfusion. The level was slightly high after 24 h of hemoperfusion. The total decrement in blood transfusion group was 33.5%~60.0%. The level was <0.3μg/L in all 25 out-patients 1 year after their intoxication. Conclusion Routine comprehensive treatment,hemoperfusion,blood transfusion are effective in the treatment of acute tetramine intoxication. The degradation of tetramine in human body is slow. 相似文献
95.
96.
陈金东 《医学分子生物学杂志》1993,(4)
EB病毒不能在其转化的淋巴细胞中引起大量溶解性感染,因此采用常规的方法分离EB病毒DNA比较困难。以往的分离方法需要从大量的部分感受态的B-淋巴细胞培养物中增殖病毒粒子,故分离到的纯EB DNA量很少(每10~(10)个细胞中只获得1 相似文献
97.
分别以改进的蛋白酶K-酚-氯仿抽提法和异硫氰酸胍-酚-氯仿-步抽提法提取肠道病毒RNA用于逆转录聚合酶链反应(RT-PCR),经8次反复试验,证明两种方法提取的肠道病毒RNA均能用于RT-PCR反应,但异硫氰酸胍-酚-氯仿法更稳定、可靠、简单快速,能满足流行病学研究中同时处理大量标本的需要。 相似文献
98.
99.
100.
用荧光分光光度法测定了济南市城区和农村0~60岁健康人群头发发样919份,硒平均值为0.62±0.24μg/g。并探讨了其含量与年龄、地区的关系。 相似文献