快速提取肠道病毒RNA用于逆转录聚合酶链反应

分别以改进的蛋白酶Ｋ－酚－氯仿抽提法和异硫氰酸胍－酚－氯仿一步抽提法提取肠道病毒ＲＮＡ用于逆转录聚合酶链反应（ＲＴ－ＰＣＲ），经８次反复试验，证明两种方法提取的肠道病毒ＲＮＡ均能用于ＲＴ－ＰＣＲ反应，但异硫氰酸胍－酚－氯仿法更稳定、可靠、简单快速，能满足流行病学研究中同时处理大量标本的需要。     

电感耦合等离子体质谱法同时测定血清金属元素的方法学研究

[目的]建立电感耦合等离子体法(ICP-MS)直接稀释测定血清中金属元素的准确分析方法。[方法]采用0.1%Trition-X-100+0.5%NH4OH对血清稀释10倍后直接用ICP-MS进行分析,用Ge内标校正基体干扰和信号漂移,采用外标法进行仪器的工作曲线校准,碰撞池技术消除质谱干扰。[结果]方法的检出限为0.001～0.392μg/L,日内精密度优于5.0%,日间精密度优于15.0%,回收率为85.0%～115.0%,与AAS、AF比较分析结果令人满意。[结论]可用碰撞池ICP-MS直接稀释测定人血清中9种元素,该方法具有样品用量少、操作简便、污染少、多元素快速准确测定的优点。     

石墨炉原子吸收光谱法测定阿法替尼中镍的残留量

目的建立微波消解-石墨炉原子吸收光谱法测定阿法替尼中镍残留量的方法。方法采用MARS 6微波消解仪和HNO3消化体系设置快速彻底消化样品,采用Zeenit650P石墨炉原子吸收光谱仪建立了阿法替尼中镍的残留量分析方法。结果镍含量在0.01μg/ml~0.10μg/ml时具有良好的线性关系,其相关系数(r)为0.998 6。仪器检出限与定量限分别为0.001 7μg/ml和0.005 7μg/ml。称样量在0.25 g时,该方法检出限为0.068 mg/kg,定量限为0.228 mg/kg。相对标准偏差为1.76%~7.10%,回收率为91.2%~107.0%。结论该法灵敏度高、干扰少,具有较强实用性,便于推广应用。     

石墨炉原子吸收光谱法测定二甲苯磺酸拉帕替尼中钯的残留量

目的建立微波消解-石墨炉原子吸收光谱法测定二甲苯磺酸拉帕替尼中钯残留量的方法。方法采用MARS6微波消解仪和HNO3消化体系设置消解程序,快速彻底消化样品,通过Zeenit650P石墨炉原子吸收光谱仪建立了二甲苯磺酸拉帕替尼中钯残留量的分析方法。结果钯元素的浓度范围为0μg/L~100μg/L时具有良好的线性关系,线性相关系数为0.999 3。方法的检出限与定量限分别为1.2μg/L和4.2μg/L。相对标准偏差为1.68%~4.96%,回收率为98.0%~113.2%。结论该方法的灵敏度高,干扰少,具有较强实用性,便于推广应用。     

间歇性双侧完全鼻阻塞对幼年大鼠下颌骨生长发育的影响

目的探讨间歇性双侧完全鼻阻塞对幼年大鼠下颌骨生长发育的影响。方法 30只3周龄SD大鼠,分为3组,A组:对照组,B组:单侧鼻阻塞组,C组:双侧鼻阻塞组(张口呼吸组),每组各10只。4周后处死30只大鼠,分离大鼠的下颌骨,在X线片上测量10项下颌骨的指标。结果双侧鼻阻塞组与对照组及单侧鼻阻塞组相比,下颌骨及股骨明显小,差异有统计学意义。单侧鼻阻塞组与对照组相比仅下颌体长度、下颌磨牙高度、股骨长度有统计学差异。结论间歇性双侧完全鼻阻塞可以引起大鼠下颌骨的发育不足。     

正畸治疗中阻生牙助萌术1例

目的 以正畸治疗中阻生牙助萌术为例,探讨正畸患者的牙周手术设计中应注意的问题。诊治过程：25岁男性患者,牙龈呈炎症表现,牙列不齐,右上颌侧切牙阻生。前牙深覆牙合,双侧第一磨牙及尖牙远中关系,右上颌中切牙和尖牙间存在间隙。前期通过正畸治疗排齐牙列,并开辟右上颌侧切牙处的间隙。在完成牙周基础治疗的前提下,设计偏腭侧切口,在有效暴露阻生牙的前提下尽可能保存所有软组织,同时根据正畸牙移动的目标位置行骨增量和软组织增量,术后牵引并排齐右上颌侧切牙。结果 通过合理的牙周手术设计,本病例在正畸过程中保持了牙周软硬组织的健康。结论 正畸患者的牙周手术设计应同时关注正畸需求和牙周健康。     

毒鼠强浓度在人体内变化规律研究

Objective To study the variation of blood concentration of tetramine in human body with acute tetramine intoxication treated with three different protocols and the levels in human body after discharge from the hos-pital. Methods The blood concentration of tetramine was determined by gas chromatography-mass spectrometry (GC-MS). All 101 patients of acute tetramine intoxication were divided into 3 groups (routine comprehensive treat-ment group,hemoperfusion group,blood transfusion group) according to their blood concentration of tetramine and clinical symptoms. The patients were followed up to monitor the tetramine levels in a year. Results The level of tet-famine in blood was decreased from 33.0(1.7～115.0)μg/L to 18.0(0.3～47.6)μg/L in routine comprehensive treatment group. The total decrement was 45.5 %. The level was decreased from 108.0 (54.0～290.0)μg/L to 26.0 μg/L in hemoperfusion group. The total decrement was 75.9%. The decrement was 20.0%～45.0% after each he-moperfusion. The level was slightly high after 24 h of hemoperfusion. The total decrement in blood transfusion group was 33.5%～60.0%. The level was <0.3μg/L in all 25 out-patients 1 year after their intoxication. Conclusion Routine comprehensive treatment,hemoperfusion,blood transfusion are effective in the treatment of acute tetramine intoxication. The degradation of tetramine in human body is slow.     

应用半巢式PCR检测滤泡性淋巴瘤患者骨髓与外周血bcl—2／JH…

为探讨非霍奇金淋巴瘤（ＮＨＬ）患有髓与外周血ｂｅｌ－２／ＪＨ基因重排的临床意义，应用半巢式多聚酶链反应（ＰＣＲ）检测１０例滤泡性淋巴瘤（ＦＬ）患者骨髓与外周血标本ｂｅｌ－２／ＪＨ基因重排。结果检出６例存在ｂｃｌ－２／ＪＨ融合基因。骨髓细胞形态检查发现的２例淋巴绞细胞浸润者均检出该融合基因，另８例骨髓细胞形态学检查正常者中４例ＰＣＲ检查阳性，其中临床分期为Ⅱ期者１例，Ⅲ期者１例。因此，检测ＦＬ患者ｂ     

毒鼠强浓度在人体内变化规律研究

Objective To study the variation of blood concentration of tetramine in human body with acute tetramine intoxication treated with three different protocols and the levels in human body after discharge from the hos-pital. Methods The blood concentration of tetramine was determined by gas chromatography-mass spectrometry (GC-MS). All 101 patients of acute tetramine intoxication were divided into 3 groups (routine comprehensive treat-ment group,hemoperfusion group,blood transfusion group) according to their blood concentration of tetramine and clinical symptoms. The patients were followed up to monitor the tetramine levels in a year. Results The level of tet-famine in blood was decreased from 33.0(1.7～115.0)μg/L to 18.0(0.3～47.6)μg/L in routine comprehensive treatment group. The total decrement was 45.5 %. The level was decreased from 108.0 (54.0～290.0)μg/L to 26.0 μg/L in hemoperfusion group. The total decrement was 75.9%. The decrement was 20.0%～45.0% after each he-moperfusion. The level was slightly high after 24 h of hemoperfusion. The total decrement in blood transfusion group was 33.5%～60.0%. The level was <0.3μg/L in all 25 out-patients 1 year after their intoxication. Conclusion Routine comprehensive treatment,hemoperfusion,blood transfusion are effective in the treatment of acute tetramine intoxication. The degradation of tetramine in human body is slow.