自制补肾降浊贴治疗慢性肾功能不全(脾肾气虚型)的64例临床研究

目的观察自制补肾降浊贴治疗慢性肾功能不全(脾肾气虚)的疗效。方法将64例肾功能不全(2-4期)患者,随机分为治疗组32例和对照组32例。治疗组及对照组常规治疗均给予口服海坤肾喜胶囊2粒日3次。治疗组采用外用中药贴剂自制补肾降浊贴贴敷肾俞、脾俞、神阙穴,对照组将热熔胶淀粉调制成安慰剂贴敷相同穴位。两组疗程均为4周。观察慢性肾功能不全的中医证候疗效研究。并检测治疗前后尿素(BUN),血肌酐(Scr)及eGFR指标变化。结果治疗4周后,治疗组总有效率为90.6%,对照组总有效率为75.0%;治疗组治疗前与治疗后Scr、BUN及e GFR均有统计学差异(P0.05),而对照组治疗前后无统计学差异(P0.05)。结论自制补肾降浊贴既能改善慢性肾功能不全患者临床症状,又能改善肾功能,并且无明显不良反应,耐受性好。对慢性肾功能不全有较好的治疗作用。     

急症中承气汤证的辨证施治

承气汤证是常见的中医急症之一,承气汤用于通下热结,为攻下峻药:用之恰当则能立杆见影,转危为安;用之不当则易伤正,加重病情,临证要谨慎施治。1 承气汤证的的辩证要点 承气汤证载于《伤寒论》,其证候表现有:“伤寒吐后,腹胀满者,与调胃承气汤”,“阳明病,不吐、不下、心烦者,可与调胃承气汤”;“阴明病,其人多汗,以津液外出,胃中燥,大使必硬,硬则谵语,小承气汤主之”,“阳阴病,谵语,发潮热,脉滑而疾者,小承气汤主之”,“二阳并病,太阳证     

依托科技创新发展三七产业

三七不仅是传统重要的中药材,且可再生,适用于工业化生产的特色生物资源.本文通过对三七研究开发状况进行分析讨论,提出依托科技创新发展三七产业的思路和建议.     

间歇性完全鼻阻塞对幼年大鼠髁突软骨细胞凋亡影响的研究

目的 研究幼年大鼠在双侧间歇性鼻阻塞情况下双侧髁突软骨细胞的凋亡情况,来尝试探讨间歇性鼻阻塞对张口呼吸儿童髁突软骨发育的影响。方法 将20只4周龄大鼠（Sprague-Dawley rat,SD rat）分为2组,A组：双侧鼻阻塞组,常氧条件下鼻孔双侧阻塞8 h（8.00AM-16.00PM）,持续35 d;B组：对照组 ,常氧条件下饲养 。2组给予同样的饮食以及饮水,然后分别取出双侧髁突,用甲醛保存做成石蜡切片,同时进行caspase-3以及Bcl-2,Bax蛋白免疫组化染色。以观察在双侧鼻阻塞对髁突前斜面中上部软骨细胞增殖以及凋亡的影响,以及双侧间歇性鼻阻塞情况下Bcl-2基因家族的调控机制以及与下颌骨生长发育的关系。结果 在间歇性双侧完全鼻阻塞情况下,SD幼年大鼠双侧髁突软骨细胞凋亡较对照组明显增加。结论 双侧鼻阻塞的未成年大鼠前斜面中上部髁突细胞Bcl-2蛋白以及Bax蛋白,Caspase-3, Bax蛋白表达均较对照组增加,Bcl-2蛋白表达数量实验组较对照组表达减少,髁突软骨细胞凋亡数量较对照组明显增加。     

毒鼠强浓度在人体内变化规律研究

Objective To study the variation of blood concentration of tetramine in human body with acute tetramine intoxication treated with three different protocols and the levels in human body after discharge from the hos-pital. Methods The blood concentration of tetramine was determined by gas chromatography-mass spectrometry (GC-MS). All 101 patients of acute tetramine intoxication were divided into 3 groups (routine comprehensive treat-ment group,hemoperfusion group,blood transfusion group) according to their blood concentration of tetramine and clinical symptoms. The patients were followed up to monitor the tetramine levels in a year. Results The level of tet-famine in blood was decreased from 33.0(1.7～115.0)μg/L to 18.0(0.3～47.6)μg/L in routine comprehensive treatment group. The total decrement was 45.5 %. The level was decreased from 108.0 (54.0～290.0)μg/L to 26.0 μg/L in hemoperfusion group. The total decrement was 75.9%. The decrement was 20.0%～45.0% after each he-moperfusion. The level was slightly high after 24 h of hemoperfusion. The total decrement in blood transfusion group was 33.5%～60.0%. The level was <0.3μg/L in all 25 out-patients 1 year after their intoxication. Conclusion Routine comprehensive treatment,hemoperfusion,blood transfusion are effective in the treatment of acute tetramine intoxication. The degradation of tetramine in human body is slow.     

莘县农村成年妇女营养知识、态度及膳食行为调查

[目的]了解影响农村成年妇女营养知识、态度及膳食行为的因素,为制定营养教育规划提供依据.[方法]1999年5～6月,采用整群抽样方法抽取莘县燕店乡6个村庄作为调查点,抽取调查点20%的住户,调查20～55岁的家庭主妇.[结果]农村社区成年妇女营养知识得分与文化程度呈正相关,与年龄呈负相关,相关均有统计学意义(r＝0.385、-0.169,P<0.01);调查对象对学习营养知识持积极肯定的态度,食物消费以面粉、蔬菜为主;电视、广播和报刊是获得营养知识的主要信息来源.[结论]提高农村成年妇女的文化程度,对于提高人群的营养知识、态度和膳食行为有着重要的作用.     

毒鼠强浓度在人体内变化规律研究

Objective To study the variation of blood concentration of tetramine in human body with acute tetramine intoxication treated with three different protocols and the levels in human body after discharge from the hos-pital. Methods The blood concentration of tetramine was determined by gas chromatography-mass spectrometry (GC-MS). All 101 patients of acute tetramine intoxication were divided into 3 groups (routine comprehensive treat-ment group,hemoperfusion group,blood transfusion group) according to their blood concentration of tetramine and clinical symptoms. The patients were followed up to monitor the tetramine levels in a year. Results The level of tet-famine in blood was decreased from 33.0(1.7～115.0)μg/L to 18.0(0.3～47.6)μg/L in routine comprehensive treatment group. The total decrement was 45.5 %. The level was decreased from 108.0 (54.0～290.0)μg/L to 26.0 μg/L in hemoperfusion group. The total decrement was 75.9%. The decrement was 20.0%～45.0% after each he-moperfusion. The level was slightly high after 24 h of hemoperfusion. The total decrement in blood transfusion group was 33.5%～60.0%. The level was <0.3μg/L in all 25 out-patients 1 year after their intoxication. Conclusion Routine comprehensive treatment,hemoperfusion,blood transfusion are effective in the treatment of acute tetramine intoxication. The degradation of tetramine in human body is slow.     

毒鼠强浓度在人体内变化规律研究

Objective To study the variation of blood concentration of tetramine in human body with acute tetramine intoxication treated with three different protocols and the levels in human body after discharge from the hos-pital. Methods The blood concentration of tetramine was determined by gas chromatography-mass spectrometry (GC-MS). All 101 patients of acute tetramine intoxication were divided into 3 groups (routine comprehensive treat-ment group,hemoperfusion group,blood transfusion group) according to their blood concentration of tetramine and clinical symptoms. The patients were followed up to monitor the tetramine levels in a year. Results The level of tet-famine in blood was decreased from 33.0(1.7～115.0)μg/L to 18.0(0.3～47.6)μg/L in routine comprehensive treatment group. The total decrement was 45.5 %. The level was decreased from 108.0 (54.0～290.0)μg/L to 26.0 μg/L in hemoperfusion group. The total decrement was 75.9%. The decrement was 20.0%～45.0% after each he-moperfusion. The level was slightly high after 24 h of hemoperfusion. The total decrement in blood transfusion group was 33.5%～60.0%. The level was <0.3μg/L in all 25 out-patients 1 year after their intoxication. Conclusion Routine comprehensive treatment,hemoperfusion,blood transfusion are effective in the treatment of acute tetramine intoxication. The degradation of tetramine in human body is slow.     

毒鼠强浓度在人体内变化规律研究

目的 探讨3种方法治疗急性毒鼠强中毒后不同时期患者体内毒鼠强浓度的改变及出院后正常生活条件下毒鼠强浓度变化.方法 用气相色谱-质谱分析患者血液中毒鼠强浓度,根据101例患者血液中毒鼠强浓度高低和临床症状分为常规综合治疗组、加行血液灌流组、加行输血治疗组,监测其治疗前后体内毒鼠强浓度变化,对出院患者进行为期1年的跟踪随访,检测其体内毒鼠强浓度变化.结果 常规综合治疗组治疗后毒鼠强浓度由治疗前33.0(1.7～115.0)μg/L下降为18.0(0.3～47.6)μg/L,平均下降45.5%;加行血液灌流组由治疗前108.0(54.0～290.0)μg/L经多次血液灌流后下降为26.0μg/L,末次血液灌流后较血液灌流前下降75.9%,每次血液灌流后下降20.0%～45.0%,血液灌流24 h后毒鼠强浓度略有回升现象;加行输血治疗患者2例,治疗后毒鼠强浓度分别下降33.5%及60.0%.25例出院患者在中毒6个月时有2例体内仍含有毒鼠强,且仍以化学原形存在,随访1年时均未再检出毒鼠强.结论 常规综合治疗、血液灌流、输血治疗毒鼠强中毒是积极有效的方法,毒鼠强在人体内代谢缓慢.     

高效液相色谱法测定保健食品中芍药甙

白芍是毛茛科植物芍药的干燥根，具有养血柔肝，缓中止痛，敛阴收汗的功能［１］。其主要成分是芍药甙［２－４］。近年来，添加白芍的保健食品愈来愈多。经文献检索，目前尚无规定的标准检验方法。为保证此类产品的质量，本文采用高效液相色谱法（ＨＰＬＣ），以甲醇──水为流动相，Ｃ１８反相色谱柱分离，ＵＶ２３２．８ｎｍ处检测芍药甙，取得了良好的效果。该法具有分辨率、灵敏度高，线性关系好，回收率高等特点，适用于以白芍为主要原料的保健食品中功效成分芍药甙的含量测定。１实验部分１．１仪器 Ｗａｔｅｒｓ高效液相色谱仪（包…