“通经接气”法浅谈

通经接气法是行针促其“得气”的一种手法。本法与飞经走气法的联系甚为密切,运用熟练时,完全可以相互依赖,融为一体。一般说:过关过节催经运气时,多运用飞经走气法,按金元时期飞经走气法常用的有白虎摇头、青龙摆尾、苍龟探穴、赤凤摇头(迎源)四种。这些手法,在历代医著中均详细论述,因与通经接气法有密切联系,所以略为一提,不作多述。这里主要谈“通经接气”法。《金针赋》说:“通经接气之法,已有定息寸数。手足三阳,上九而下十四,过经四寸。手足三阴,上七而下十二,过经五寸。在乎摇动出纳,呼吸同法,驱运气血,倾刻周流,上下通接,可使寒者暖而热者凉,痛者止而胀者消。若开渠之决水,立时见功,何倾危之不起哉。”通经接气法是根据定息寸数的规律行针的。《流注指微赋》中窦氏以一句话说明:“接气通经,短长依法。里外之绝,羸盈必别。”这是通经接气的运针规律。     

巨细胞病毒糖蛋白B基因型与新生儿感染关系研究

目的：探讨感染新生儿人巨细胞病毒（HCMV）糖蛋白B（gB）基因型分布，研究不同HCMVgB基因型致病性的差异。方法：采用巢式聚合酶链反应（nested PCR），限制性片段长度多态性（RFLP）分析技术等测定88例感染患儿尿中HCMV gB基因型，并分析gB基因型与不同临床症状的关系。结果：感染新生儿HCMV4个gB基因型分布为gB1型占48％，gB2型占舻％，gB3型占18％，gB4型占7％。不同gB基因型HCMV感染的临床表现存在差异。结论：4个gB基因型HCMV均能引起新生儿感染，以gB1型为主。不同HCMVgB型感染可导致不同的临床结果。     

薄膜过滤法应用在丝虫病普查中组成混合标本...

The membrane filtration technique has been used widely in the evaluation of effect of control and survey of filariasis. The present study was made to explore an optimum number of mixed peripheral blood samples and a mathematical model of work load for this method in surveying filariasis. By analysing the correlation between the microfilaremia rate and the optimum number of mixed peripheral blood samples and applying the theory of Binomial Distribution or Poisson Distribution, the authors reckoned a table for estimating the number of filariasis cases in villages with different microfilarial rates and different population as well as the optimum number of mixed peripheral blood samples.     

鲍曼不动杆菌耐药性及β-内酰胺酶基因型动态变化研究

目的 调查分析聊城市309株鲍曼不动杆菌耐药性及β-内酰胺酶基因型.方法 用K-B法测定临床分离的309株鲍曼不动杆菌对13种抗菌剂的耐药性,以Nitrocefin纸片法和改良三维试验大平皿法对β-内酰胺酶及分型进行检测,采用聚合酶链反应(PCR)检测β-内酰胺酶耐药基因型.结果 除亚胺培南(IMP)外,309株鲍曼不...     

乳腺良恶性钙化的血清比较蛋白质组学分析

目的 分析乳腺良恶性钙化患者血清蛋白质表达谱,比较不同性质钙化的血清蛋白质差异.方法 钙化型乳腺癌、良性钙化患者血清各10例,经双向凝胶电泳、基质辅助激光解析离子化-飞行时间质谱等对差异蛋白进行分析鉴定和验证.结果 良恶性钙化组比较发现钙化型乳腺癌组血清中特异性的蛋白质2种,上调的蛋白质8种,下调的蛋白质7种.钙化型乳腺癌组触珠蛋白表达高于良性钙化组.结论 乳腺的恶性钙化和良性钙化患者的血清中某些蛋白存在着差异表达,有可能作为鉴别乳腺钙化病变良恶性质的标志物分子.

Abstract：

Objective To find out serum protein difference between breast benign calcification and malignant calcification.Methods The serum samples of the patients with breast cancer and those with benign lesions were collected,10 samples in each category.They all had calcifications on mammygraphy.The mixed protein was assayed by using 2-DE method.The spots with marked different expression were excised from gels and digested into peptides.These peptides were analyzed by MALDI-TOF MS and the peptide mass fingerprintings were got.One of special protein was valided by Western blotting.Results In group of breast cancer with calcification,there were 2 specific proteins.There were 15 differential expression proteins,including 8 up-regulated,and 7 down-regulated.Conclusion Some identified serum proteins have different expression in patients with malignamt or benign calcifications,which may be helpful for the differential diagnosis of benign and malignant calcifications.     

乌司他丁治疗妇科低血容量性休克临床观察

目的探讨乌司他丁对妇科低血容量性休克患者肾功能的保护作用,及其可能的作用机制。方法妇科失血性低血容量性休克病例50例,随机分为治疗组和对照组,每组25例。两组均立即补液、输血抗休克治疗,同时急诊手术去除出血病灶,治疗组同时还加用乌司他丁2000U/kg.次,1次/8小时,共6次。结果两组术前TXA2/PGI2比值、IL-6、TNF-α、Scr、BUN没有明显差异（均P〉0.05）。术后24h内,治疗组TXA2/PGI2比值均低于对照组（P〈0.05）;术后48h内,治疗组IL-6、TNF-α含量均低于对照组（均P〈0.05）。术后,治疗组Scr、BUN恢复明显快于对照组（均P〈0.05）。结论乌司他丁对妇科低血容量性休克患者的肾功能有一定的保护作用,调节患者外周血TXA2/PGI2比值和IL-6、TNF-α含量,可能是其保护肾功能的主要机制。     

心理干预对产妇分娩的影响

目的探讨心理干预对产妇分娩过程的影响。方法212例足月妊娠无阴道分娩禁忌证的待产妇按入院单双号随机分成观察组（106例）和对照组（106例）,观察组给予针对性心理干预和心理疏导,对照组给予产前常规知识宣教,观察两组产程时间、分娩方式、产后出血量等。结果观察组与对照组比较产程缩短、剖宫产率降低、产后出血量减少,两组差异均有统计学意义（均P〈0．05）。结论有效的心理干预能显著提高产科质量。     

颈内动脉闭塞时同侧颈总动脉血流频谱特点分析

目的探讨单侧颈内动脉闭塞时,同侧颈总动脉血流频谱改变的特点。方法采用彩色多普勒超声诊断仪对63例单侧颈内动脉闭塞的患者进行同侧颈总动脉血流频谱分析,测量颈总动脉收缩期峰值流速,舒张末期流速,搏动指数,阻力指数等指标。结果单侧颈内动脉闭塞的患者同侧颈总动脉的血流速度明显较无病变侧的降低,搏动指数及阻力指数均较无病变侧显著增高。结论颈总动脉血流频谱的异常改变可以间接估测颈内动脉病变程度,对颈内动脉是否闭塞的判定有一定的诊断价值。