阻断乙型肝炎母婴传播的初步研究

本文报告了四川地区阻断乙型肝炎母婴传播的研究结果。筛检孕妇HBsAg和HBeAg双阳性,或HBsAg阳性效价≥1:512的孕妇所产新生儿作为研究对象。80例新生儿按随机、双盲、安慰剂对照原则分成3组:疫苗组、疫苗加HBIG组和对照组。随访观察12个月时,乙肝感染保护率分别为88.0%和92.0%。认为国产乙肝疫苗和HBIG阻断乙肝母婴传播效果较好,单独疫苗接种效果亦较满意。     

人体标本陈列室的环境建设与管理

人体标本陈列室是人体解剖学实验室的一部分,也是学校及解剖学教研室对外进行学术交流的一个窗口.长期以来,由于人体标本陈列室的建设投入大,又不产生直接的经济效益而不被学校领导重视,因此,大多数院校的人体标本陈列室用房陈旧,室内设施不完善,甲醛溶液刺激性气味重,陈列的标本疏于管理,更不注重更新,标本柜也已破旧不堪了[1].     

超声引导下髂动脉破裂出血模型的建立

目的 探讨超声引导下使用一次性穿刺活检装置制备髂动脉破裂出血模型的可行性.方法 选用实验动物五指山猪9只,均为雄性,体质量为(14.06±0.31)kg.超声引导下经颈内静脉穿刺留置中心导管给予限量补液及麻醉维持.经颈总动脉穿刺置管并连接压力换能器,通过生物信号采集处理系统连续监测有创动脉血压和心率.在超声引导下使用一...     

新冠病毒受体蛋白ACE2结构与功能的生信分析及原核表达

目的：构建ACE2原核表达载体并对其进行结构与功能的生物信息学分析，探究其介导SARS-CoV-2入侵人体的分子机制。方法：采用Protparam、ProtScale、NetPhos3.1、SignaIP 4.1、YinOYang 1.2 Server、NetNGlyc 1.0 Server、SOPMA、SWISS-MODEL、Blast、Clustal X2和MEGA7.0等对ACE2的生物学特性、同源性及进化性进行系统分析。采用分子克隆技术构建pET-22b-ACE2，在大肠杆菌中进行表达。结果：ACE2全长805个残基，呈酸性，分子量为92.5 kD,pI=5.36，共含77个潜在的磷酸化位点和14个糖基化位点，是一种亲水性较强的跨膜蛋白。ACE2主要散布于内质网膜、高尔基体和胞质膜，二级结构组成以α-螺旋为主。原核表达结果显示，细菌裂解后，沉淀中ACE2表达多于上清与全菌，为疫苗研发提供了理论依据。多序列比对和进化分析显示，倭黑猩猩与智人亲缘关系最近。结论：本研究为ACE2蛋白的纯化及研究奠定了基础，有助于阐明ACE2介导SARS-CoV-2入侵人体的分子机制，对SARS-Co...     

乳腺癌辅助化疗后预防性使用乙二醇化重组人粒细胞刺激因子的成本效果分析和对生活质量的影响

目的 探究乳腺癌辅助化疗后预防性使用聚乙二醇化重组人粒细胞刺激因子（PEG-rhG-CSF）的成本效果及对生活质量的影响。方法 本研究是一项前瞻性队列研究。纳入我科乳腺癌辅助化疗的患者,根据是否预防性使用PEG-rhG-CSF分组,并进行血常规监测。比较两组化疗间期重度粒细胞减少发生率、延迟化疗率、成本费用指标及生活质量,计算增量成本效果比（Incremental cost benefit ratio,ICER）。结果 共256例患者纳入分析。与对照组相比,PEG-rhG-CSF组重度中性粒细胞减少和延迟化疗的发生率较低,生理状况方面生活质量更好,总费用稍高,针对重度粒细胞减少发生率和延迟化疗率的ICER分别为25.32元/1%和24.45元/1%。结论 乳腺癌辅助化疗后预防性使用PEG-rhG-CSF效果良好并提高生活质量,性价比较高。     

Effect of a novel phosphodiesterase type 5 inhibitor,CPD1, on carbon tetrachloride-induced liver fibrosis in mice北大核心CSCD

Aim To investigate the effects of CPD1, a novel phosphodiesterase 5 inhibitor, on liver pathological phenotype and hepatic stellate cells (HSCs) activation in hepatic fibrosis model mice caused by carbon tetrachloride ( CCl4). Methods Male C57BL/6 J mice were divided into four groups randomly ( control group, CCl4group, CCl4+ CPD1 group and CCl4+ tadalafil group) . Hepatic fibrosis model was construc¬ted by intraperitoneal injection of CCl4( twice a week) . Four weeks after CCl4injection, the mice were treated with CPD1 (2 mg kg-1• d-1) , or Tadalafil (10 mg • kg-1• d-1) by intragastric administration, respec¬tively, for four weeks. Hematoxylin-eosin staining and Sirius Red staining were used to observe the distribu¬tion of liver tissue structural lesions and fibrosis. Im-munohistochemical staining was used to detect the ex¬pression of a-smooth muscle actin ( a-SMA) and fi-bronectin. Results Compared with control group, the liver tissue structure was seriously damaged in CCl4group with many hepatocytes necrosis and inflammatory cell infiltration, indicating that liver injury occurred in the CCl4-induced hepatic fibrosis model mice. Moreo¬ver, the expressions of a-SMA increased significantly in CCl4group. Compared to CCl4group, the liver tissue damage was significantly improved in PDE5 inhibitors group,most notably, CPD1 had a better curative effect than tadalafil did. Furthermore, CPD1 inhibited the ex¬pression of a-SMA markedly and reduced the expres-sion of ECM-related proteins induced by transforming growth factor pi ( TGF-f31 ) in Lieming Xu-2 ( LX-2 ) cells. Conclusions Phosphodiesterase 5 inhibitor CPD1 strongly alleviates CCl4-induced hepatic damage by inhibiting the activation of HSCs and expression of collagen fibers. © 2023 Publication Centre of Anhui Medical University. All rights reserved.