空军飞行员角膜内皮细胞的初步分析

Objegtive To preliminarily study the endothelial density baseline and the morphological characteristics of the corneal endothelial cell of Chinese Air Force pilot. Metheds Density and morphology of corneal endothelial cells were observed by Topcon-2000 noncontact specular microscope and analyzed by Imagenet 2000 software(version 2.53)on 56 pilots(112 eyes)While other 30 healthy men(60 eyes)who were with the comparable age and uncorrected visual acuity were compared and analyzed as the control group. Results The data showed that the average corneal endothelial cell density was 2797.0±330.3/mm2 in pilot group which was significantly lowet than 2917.57±318.04/mm2 in control group(t=2.286,P=0.024).The mean constituent ratio of hexagonal cell was 56.46±7.19 in pilot group compared with 60.33±7.68 in control group(t=8.253,P=O.001)and it was gradually reduced with the age growing in both groups(r=-0.395,-0.268,P=0.000.0.038).With the growing of flying hours the mean area of cell was increased under the eontrolled age factor according to partial correlation analysis(r=0.244,P=0.0 1).Conclusions Measuring of the corneaI endothelial cellular morphologic parameters and implementing corresponding analysis are helpful to know pilot's corneal endotheliurn changes in flight.     

垂直弹射对LASIK术后兔角膜瓣稳定性的影响

目的 研究垂直弹射对准分子激光原位角膜磨镶术（laser in situ keratomileusis,LASIK）后兔角膜瓣稳定性的影响.方法 将12只日本大耳白兔双眼行LASIK,术后1周随机分2组,一组为对照组,未行任何刺激;另一组为垂直弹射组,弹射模拟器的弹射高度为 5 m,垂直加速度为＋7 G,反复10次.用光镜和透射电镜观察兔角膜瓣结构的变化,并比较两组角膜瓣的稳定性.结果 光镜观察：弹射后角膜基质愈合线清晰,未见分离,部分基底细胞呈空泡样变性,胶原排列轻微紊乱.透射电镜观察：伤口对合紧密,周围组织修复均匀,创口两侧角膜纤维组织排列规则,未见层间分离.兔角膜瓣稳定性比较：弹射组与对照组,未见统计学差异（P=0.64）.结论 LASIK术后1周,垂直弹射10次观察兔角膜瓣稳定.     

空军飞行员角膜内皮细胞的初步分析

Objegtive To preliminarily study the endothelial density baseline and the morphological characteristics of the corneal endothelial cell of Chinese Air Force pilot. Metheds Density and morphology of corneal endothelial cells were observed by Topcon-2000 noncontact specular microscope and analyzed by Imagenet 2000 software(version 2.53)on 56 pilots(112 eyes)While other 30 healthy men(60 eyes)who were with the comparable age and uncorrected visual acuity were compared and analyzed as the control group. Results The data showed that the average corneal endothelial cell density was 2797.0±330.3/mm2 in pilot group which was significantly lowet than 2917.57±318.04/mm2 in control group(t=2.286,P=0.024).The mean constituent ratio of hexagonal cell was 56.46±7.19 in pilot group compared with 60.33±7.68 in control group(t=8.253,P=O.001)and it was gradually reduced with the age growing in both groups(r=-0.395,-0.268,P=0.000.0.038).With the growing of flying hours the mean area of cell was increased under the eontrolled age factor according to partial correlation analysis(r=0.244,P=0.0 1).Conclusions Measuring of the corneaI endothelial cellular morphologic parameters and implementing corresponding analysis are helpful to know pilot's corneal endotheliurn changes in flight.     

空军飞行员角膜内皮细胞的初步分析

Objegtive To preliminarily study the endothelial density baseline and the morphological characteristics of the corneal endothelial cell of Chinese Air Force pilot. Metheds Density and morphology of corneal endothelial cells were observed by Topcon-2000 noncontact specular microscope and analyzed by Imagenet 2000 software(version 2.53)on 56 pilots(112 eyes)While other 30 healthy men(60 eyes)who were with the comparable age and uncorrected visual acuity were compared and analyzed as the control group. Results The data showed that the average corneal endothelial cell density was 2797.0±330.3/mm2 in pilot group which was significantly lowet than 2917.57±318.04/mm2 in control group(t=2.286,P=0.024).The mean constituent ratio of hexagonal cell was 56.46±7.19 in pilot group compared with 60.33±7.68 in control group(t=8.253,P=O.001)and it was gradually reduced with the age growing in both groups(r=-0.395,-0.268,P=0.000.0.038).With the growing of flying hours the mean area of cell was increased under the eontrolled age factor according to partial correlation analysis(r=0.244,P=0.0 1).Conclusions Measuring of the corneaI endothelial cellular morphologic parameters and implementing corresponding analysis are helpful to know pilot's corneal endotheliurn changes in flight.     

胃癌细胞的体外药敏试验与多药耐药基因表达的关系

目的:分析胃癌细胞的体外药敏试验与多药耐药基因的表达之间的相关性。方法:采用MTT法测定化疗药物对胃癌肿瘤细胞的敏感性;用逆转录聚合酶链反应(RT-PCR)测定胃癌组织中多药耐药基因(MDR1,MRP1,ABCG2,Topo-2mRNA)的表达水平。结果:单药组抑制率最高为5-FU(36.51%),联合用药组抑制率最高为DCF(56.80%);多药耐药基因(MDR1,MRP1,ABCG2,Topo-2mRNA在胃癌组织中的阳性率分别为33.3%,42.9%,52.4%,66.7%。结论:体外药敏试验测定并结合多药耐药基因表达的相关性可提高预测肿瘤的敏感性或发现其耐药性,为临床实现个体化治疗提供参考依据。     

基于网络药理学和分子对接探究姜黄素治疗糖尿病视网膜病变的药效机制

目的应用网络药理学及分子对接的相关理论方法探究姜黄素治疗糖尿病视网膜病变(DR)的作用机制。方法利用SEA及SwissTargetPrediction数据库在线预测化合物作用的靶点;通过CTD数据库提供与疾病相关的各种靶点;运用Venny数据库映射匹配不同基因, 并取二者交集;采用GeneMANIA数据库构造出交集基因的蛋白互作网络图, 采用Cystoscape软件进行更精确的分析和可视化, 借助WebGestalt数据库进行富集分析, 最后利用AutoDock Vina对核心靶点进行分子对接。结果得到姜黄素作用靶点52个, DR对应的靶点1 599个, 共同作用的靶点48个。核心靶点为丝氨酸/苏氨酸-蛋白激酶1(AKT1)、肿瘤坏死因子α(TNF-α)、表皮生长因子受体(EGFR)、信号转导及转录激活因子3(STAT3)以及热休克蛋白90α家族A类成员1(HSP90AA1)。富集分析结果显示, 这些靶点主要与EGFR酪氨酸激酶抑制剂耐药性信号通路、缺氧诱导因子1(HIF-1)信号通路、白细胞介素-17(IL-17)信号通路以及晚期糖基化终末产物-晚期糖基化终末产物受体(AGE-RAG...     

65例异位妊娠伴失血性休克患者抢救成功体会

目的：探讨对在异位妊娠大量出血致失血性休克的抢救经验。方法：回顾性分析65例异位妊娠大量内出血伴失血性休克患者的临床资料。结果：65例患者全部抢救成功。结论：在抗休克同时及时正确诊断,尽早手术,尽量争取回输自体血是成功抢救异位妊娠大量内出血伴失血性休克患者的重要措施。     

美国空军飞行人员角膜屈光手术的特许评估

鉴于美国空军飞行人员屈光不正的高发生率及戴镜飞行的安全隐患,经过大量前期实验研究与评估,美国空军批准应用角膜屈光手术（corneal refractive surgery,CRS）改善现役飞行员视力状况。从2000年8月批准在航空和特种作业人员中实行准分子激光屈光性角膜切削术（photorefractive keratectomy,PR K）项目[1],2001年项目全面展开[2]。从2006年开始对部分机种飞行人员获准实行准分子激光原位角膜磨镶术（laser assisted in situ keratomileusis,LASIK）,2007年后对所有飞行人员实行LASIK特许[3]。     

先天性鼻泪管阻塞首次探通失败再治疗的临床分析

目的：分析我院就诊的28例首次泪道探通失败的先天性鼻泪管阻塞病例资料,总结再治疗策略。

方法：回顾性研究,选取2013-02-01/2017-12-31在我院就诊的591例患儿中的28例,均为首次泪道探通失败的先天性鼻泪管阻塞患儿(不包括外伤性和先天性泪道畸形),收集病史、鼻腔情况、首次探通年龄,治疗前后情况等病例资料。

结果：患儿28例中,15例经再次泪道探通联合置管术,取管后已治愈; 10例患儿经下鼻甲处理后,再次泪道探通联合置管术,取管后已治愈; 2例患儿经常发作急性泪囊炎,在全身麻醉下行鼻内窥镜下鼻腔泪囊吻合术; 1例骨性鼻泪管狭窄和阻塞患儿,有溢泪不伴溢脓,建议定期随访,13岁后鼻窦发育完全后行鼻内窥镜下鼻腔泪囊吻合术。

结论：先天性鼻泪管阻塞首次泪道探通失败再治疗需采取个性化的阶梯治疗方案,合理治疗该疾病。