Electro-acupuncture up-regulates the expression of stromal cell-derived factor-1α mRNA and its protein and promotes revascularization in the brain after focal cerebral ischemia and reperfusion

Objective To investigate the mechanism by which electro-acupuncture (EA) promotes revascularization in the brain after focal cerebral ischemia and reperfusion.Methods The Sprague-Dawley rat model of focal cerebral ischemia was made by filament occlusion. The rats were randomly divided into a control group, a model group, and an EA group. The model and EA groups were each divided into 5 subgroups receiving reperfusion 1, 3,7, 14 or 21 days after ischemia. EA was given at the bilateral Hegn point (LI 4) in the EA group. The expression of stromal cell-derived factor-1α(SDF-1α) mRNA was detected using a RT-PCR in the 3, 7 and 14 day subgroups.The immunohistochemical method was employed to detect the expression of SDF-1α protein. Results Compared with the control group, expression of SDF-1α protein increased significantly in the model and EA groups. Compared with the model group, the expression of SDF-1α mRNA increased significantly in the 3, 7 and 14 day subgroups.SDF-1α protein expression and microvessel count increased slightly but not significantly in the 1d subgroup, but the increases were significant in the 3, 7, 14 and 21 day subgroups.Conclusions EA may promote angiogenesis in an ischemic area of the cortex by increasing the expression of SDF-1αmRNA and its protein after focal cerebral ischemia and reperfusion.     

Electro-acupuncture up-regulates the expression of stromal cell-derived factor-1α mRNA and its protein and promotes revascularization in the brain after focal cerebral ischemia and reperfusion

Objective To investigate the mechanism by which electro-acupuncture (EA) promotes revascularization in the brain after focal cerebral ischemia and reperfusion.Methods The Sprague-Dawley rat model of focal cerebral ischemia was made by filament occlusion. The rats were randomly divided into a control group, a model group, and an EA group. The model and EA groups were each divided into 5 subgroups receiving reperfusion 1, 3,7, 14 or 21 days after ischemia. EA was given at the bilateral Hegn point (LI 4) in the EA group. The expression of stromal cell-derived factor-1α(SDF-1α) mRNA was detected using a RT-PCR in the 3, 7 and 14 day subgroups.The immunohistochemical method was employed to detect the expression of SDF-1α protein. Results Compared with the control group, expression of SDF-1α protein increased significantly in the model and EA groups. Compared with the model group, the expression of SDF-1α mRNA increased significantly in the 3, 7 and 14 day subgroups.SDF-1α protein expression and microvessel count increased slightly but not significantly in the 1d subgroup, but the increases were significant in the 3, 7, 14 and 21 day subgroups.Conclusions EA may promote angiogenesis in an ischemic area of the cortex by increasing the expression of SDF-1αmRNA and its protein after focal cerebral ischemia and reperfusion.     

Cortisomol根管糊剂行一次性根管充填的临床效果评价

目的观察Cortisomol根管糊剂进行一次性根管充填的临床效果,为其推广使用提供确切依据。方法将所有病例均分2组,镍钛根管预备后,治疗组以Cortisomol根管糊剂和牙胶尖侧方加压法进行一次性根管充填,对照组以丁香油氧化锌糊剂和牙胶尖侧方加压法进行一次性根管充填。结果两组术后疼痛发生率差异有统计学意义(P<0.01),Cortisomol组术后反应轻。术后1年复查,两组疗效差异无统计学意义(P>0.05)。结论应用Cortisomol根管糊剂进行一次性根管充填,临床操作简单,术后反应低,效果显著,值得推广应用。     

铅暴露对肥胖小鼠脑组织SVZ区损伤的实验研究

目的 探究铅暴露对肥胖小鼠侧脑室下区(SVZ)区损伤状况。方法 60只SPF级C57小鼠随机分为普通饲料组和高脂饲料组，用高脂饲料诱导肥胖小鼠模型。将普通饲料组分为对照组和铅暴露组，将肥胖小鼠分为肥胖组和肥胖+铅暴露组。对照组和肥胖组给予正常饮水；铅暴露组和肥胖+铅暴露组给予250 mg/L的醋酸铅饮用水，染毒8周。应用糖水偏好实验检测小鼠抑郁行为；应用ICP-MS检测小鼠全血和SVZ中铅含量；应用Western blot检测脑源性神经营养因子(BDNF)的蛋白表达；免疫荧光共定位法检测侧脑室下区新生神经元数量；应用ELISA法检测SVZ中白介素17(IL-17)含量；应用SPSS 22.0进行统计学分析。结果 饲喂高脂饲料7周后，高脂饲料组小鼠的平均体重超过正常小鼠体重的20%,肥胖小鼠模型造模完成；染毒8周后，与对照组比较，铅暴露组、肥胖+铅暴露组小鼠血液和SVZ中的铅含量显著升高；肥胖组和铅暴露组小鼠的糖水偏好率分别为58.63%和63.63%,显著低于对照组的66.67%;与对照组比较，肥胖组和铅暴露组小鼠SVZ中新生神经元数量和BDNF蛋白表达下降；肥胖组和铅暴露组小鼠SVZ...