胸水细胞8-OH-dG,k-ras基因突变在肺癌诊断中的意义

目的探讨胸水细胞8-OH-dG,k-ras基因蛋白表达和突变与肺癌的关系.方法采集53例肺癌病人、53例非肺癌病人的胸水,离心得细胞,将细胞成分离心涂片,用免疫组化学分别测定8-OH-dG,k-ras基因的蛋白表达和用PCR-SSCP法检测胸水细胞k-ras基因突变率.结果肺癌病人胸水细胞8-OH-dG,k-ras基因的蛋白表达阳性率分别为77.4%(41/53)与56.6%(30/53);非肺癌病人胸水细胞8-OH-dG,k-ras基因的蛋白表达阳性率分别为13.2%(7/53)与3.8%(2/53),两组比较差异有高度显著性(P＜0.01);PCR-SSCP法检测肺癌病人、非肺癌病人胸水细胞,k-ras基因突变为62.3%(33/53)与5.7%(3/53),两组比较差异有高度显著性(P＜0.01).53例肺癌病人胸水细胞8-OH-dG,k-ras基因的蛋白表达分值分别为1.68±1.21与1.37±1.13,等级相关分析呈高度正相关(Rs=0.743,P＜0.01).结论肺癌病人胸水细胞,8-OH-dG,k-ras基因的蛋白表达阳性率、分值以及PCR-SSCP法检测胸水细胞k-ras基因突变率均高于非肺癌病人;肺癌组胸水细胞8-OH-dG,k-ras基因蛋白表达两者有关联.     

中药组方抑制lewis肺癌细胞转移及机理研究

目的：探讨以黄芪、补骨脂、云芝等组成的中药组方在稳定肺癌病灶、减少转移和提高生存质量等方面的意义及其分子机制。方法：复制小鼠lewis肺癌模型,检测中药组方、替加氟和生理盐水对各组肿瘤细胞PCNA表达的影响,并记数小鼠肺组织内肿瘤转移结节数量等数据,比较组间差异。结果：PNCA阳性表达率在生理盐水组显高于替加氟组和消积饮组（P＜0．01）,而后两则差异无显性（P＞0．05）;中药组和替加氟组转移瘤结节数量少于生理盐水组,差异有显性（P＜0．01）;中药组结节数量多于替加氟组,差异无显性（P＞0．05）。结论：该中药组方能有效降低小鼠lewis肺癌细胞PCNA的表达,抑制肿瘤细胞肺内转移灶的形成,表现为临床抑癌、抗癌的效果。     

Diagnosis and Surgical Treatment of Congenital Cor Triatrium

Objectives To evalu-ate the outcome of diagnosis and surgical treatment for cor triatrium (CTA) in 6 patients seen between 1994 and 2002. Methods 6 patients ranging in age from 5 months to 25 years were observed. All of them had other cardiovascular defects, and presented with dyspnea, palpitations (and low weight growing only in the infants) . Preopreative two dimensional echocardiogra-phy had demonstrated an abnormal septum in the left atrium and other coexistent anomalies. In 3 of them the membrane was obstructed between the left atrial accessory chamber and the left atrium. The communication were atrial septal defect (ASD) indirectly; The clinical findings were due to the pulmonary hypervas-cularity. Only one case had a fenestration in the septum with a small patent foramen ovale (FO) directly , and the clinical findings were due to the obstruction to flow through the membrane in the left atrium, producing venocapilar pulmonary hypertension. Two of them had ASD and fenestration on the septum. The ot     

主动脉窦瘤破裂的外科治疗

目的:总结我院1992年12月至2004年2月13例主动脉窦瘤破裂的外科治疗经验,进一步探讨其疾病特点及手术方法.方法:13例病人(男性7例、女性6例)在中低温体外循环下行主动脉窦瘤修补术,7例病人同期行室间隔缺损修补术,2例同期行房间隔缺损修补术,1例同期行主动脉瓣成形术,2例同期行主动脉瓣替换术,其他1例.阻断时间(67.46±26.13)分,体外循环时间(103.29±38.05)分.结果:本组病例无死亡.1例病人术后早期出现频发室性早搏、室性二联律,静脉滴注利多卡因有效.所有病人治愈出院,随诊无一例复发.结论:主动脉窦瘤破裂是罕见的心脏疾病,尽早手术是唯一有效的治疗方法.采用主动脉及右心房或主动脉及右心室双切口利于心肌保护和确切修补主动脉窦瘤、纠正合并畸形.     

云南省提高山区疑似肺结核患者就诊到位措施效果评价

[目的]评价3种不同激励措施对疑似肺结核患者就诊到位的影响效果。[方法]在云南省抽取12个县,分为激励1、2、3组(分别为对样本县预防控制中心结核病门诊医生追踪疑似肺结核患者进行激励、对综合医院医生转诊就诊疑似肺结核患者进行激励、在综合医院对就诊疑似肺结核患者提供现金激励)和对照组(不实施激励),比较激励措施实施前与实施期间各组患者的转诊到位与追踪到位情况。[结果]12个县合计报告疑似肺结核患者2 113例,其中干预实施前(2006年10月至2007年1月)912例,干预实施期间(2007年2～5月)1 201例。患者就诊到位率,干预前为52.74%,干预实施期间为67.78%(P<0.01)。干预1、2、3组和对照组就诊到位率,干预前分别为61.90%、62.72%、67.08%、60.59%(P>0.05),干预实施期间分别为74.65%、60.53%、77.56%、59.55%,干预1、3组均高于干预2组和对照组(P<0.01),干预2组与对照组、干预1组与干预3组的差异均无统计学意义(P>0.05)。[结论]为疾病预防控制中心结防门诊医生提供追踪激励或为疑似肺结核患者提供就诊交通补助是提高患者就诊到位率的有效措施。     

心脏不停跳心内直视手术的临床应用

目的探讨心脏不停跳心内直视手术临床应用的优点和局限性。方法回顾性分析203例心脏不停跳心内直视手术技术、手术效果及并发症。结果体外循环时间13~210min,平均(58·3±27·7)min,113例(55·7%)术后不需使用任何正性肌力药物,其余90例(44·3%)仅需小剂量多巴胺(2~6μg·kg-1·min-1)支持循环;术后呼吸机辅助呼吸时间1·5~19h,平均(7·9±5·3)h;术后血红蛋白尿24例(11·8%),多于术后3~12h恢复正常;术后发生急性肾功能不全2例(0·98%);全组无死亡,无空气栓塞,无Ⅲ度房室传导阻滞,无严重心律失常,无神经系统症状发生。结论心脏不停跳心内直视手术是一种易于施行和安全可靠的方法,在某些方面具有一定的优越性,可作为常规手术在临床应用。     

胸腔镜辅助食管癌切除术应该注意的几个问题

电视辅助胸腔镜手术(Video-Assisted Thoracoscopic Surgery．VATS)，近年来已发展成为胸外科领域一种重要的治疗手段。使用VATS行食管癌切除，具有创伤小，恢复快的优点，克服了传统开胸术须切断或切除肋骨，致使胸廓完整性遭受破坏的不足。因此．尽管用VATS方法行食管癌切除在我国开展仅有几年时间，但却有了较快的发展并取得了可喜的进步，虽然病例数量方面与传统的开胸术在目前还无法相比，     

ShRNA-mediated gene silencing of β-catenin inhibits growth of human colon cancer cell Colo205 in vitro

Objective To observe the gene silencing and disruption of WNT pathway mediated by the specific shRNA targeted against β-catenin and its effect on cell proliferation of the human colon cancer cell line Colo205. Methods The shRNA plasmid vectors against β-catenin were constructed and transfected into Colo205 ceils with LipofectamineTM 2000. The expression of β-catenin was detected by RT-PCR and Western blot. Immunofluorescence staining was also performed to detect the β-catenin protein expression in cells. The cell proliferation inhibition was determined by MTT assay and soft agar colony formation assay. Results The shRNA vectors targeted against β-catenin were successfully constructed and efficiently suppressed the expression of β-catenin mRNA and protein (P<0.05). The expression inhibition rates were 47.89% and 45.26% at the mRNA and protein level respectively. Immunofluorescence microscopy also demonstrated the inhibition of β-catenin protein induced by these specific shRNAs. The MTT assay indicated that the specific shRNA resulted in significant inhibition of cell growth on the culture plates in time-dependent manner. At 72 h post-transfection, the cell viability of CAT group was 48.5%, which was significantly different as compared with that of blank control group's 91.3%(P<0.05). In the soft agar, there were 9, 46, 43 cell colonies in the CAT, blank, and negative control groups respectively, which were significantly different (P<0.05). Conclusions The specific shRNAs targeted against β-catenin has a gene silencing effect and blocks the WNT signaling pathway, which can inhibit the growth of Colo205 cells.     

ShRNA-mediated gene silencing of β-catenin inhibits growth of human colon cancer cell Colo205 in vitro

Objective To observe the gene silencing and disruption of WNT pathway mediated by the specific shRNA targeted against β-catenin and its effect on cell proliferation of the human colon cancer cell line Colo205. Methods The shRNA plasmid vectors against β-catenin were constructed and transfected into Colo205 ceils with LipofectamineTM 2000. The expression of β-catenin was detected by RT-PCR and Western blot. Immunofluorescence staining was also performed to detect the β-catenin protein expression in cells. The cell proliferation inhibition was determined by MTT assay and soft agar colony formation assay. Results The shRNA vectors targeted against β-catenin were successfully constructed and efficiently suppressed the expression of β-catenin mRNA and protein (P<0.05). The expression inhibition rates were 47.89% and 45.26% at the mRNA and protein level respectively. Immunofluorescence microscopy also demonstrated the inhibition of β-catenin protein induced by these specific shRNAs. The MTT assay indicated that the specific shRNA resulted in significant inhibition of cell growth on the culture plates in time-dependent manner. At 72 h post-transfection, the cell viability of CAT group was 48.5%, which was significantly different as compared with that of blank control group's 91.3%(P<0.05). In the soft agar, there were 9, 46, 43 cell colonies in the CAT, blank, and negative control groups respectively, which were significantly different (P<0.05). Conclusions The specific shRNAs targeted against β-catenin has a gene silencing effect and blocks the WNT signaling pathway, which can inhibit the growth of Colo205 cells.     

患儿气道异物取出术护理

目的总结气道异物患儿围手术期护理措施及经验。方法对2006年1月-2011年4月收治的991例气道异物患儿的临床资料及护理方法进行回顾分析。结果 985例患儿1次手术取净异物,4例患儿2次手术取净异物,2例死亡。结论气道异物起病急,病情危重,术前准确、及时的评估患儿病情,采取正确、有效的护理措施,术后严密观察患儿病情变化,可减少或避免气道异物并发症的发生,降低死亡率。