川芎嗪对大鼠重型颅脑损伤后神经细胞凋亡及Bcl-2、Bax表达的影响

目的探讨川芎嗪对大鼠重型颅脑损伤后神经细胞凋亡及相关基因Bcl-2、Bax表达的影响和对脑神经的保护作用。方法120只SD健康大鼠随机分为假手术组、模型组、治疗组,其中模型组和治疗组采用Feeney自由落体撞击装置制作大鼠重型颅脑损伤模型,治疗组给予盐酸川芎嗪,用TUNEL及免疫组化法检测三组间细胞凋亡及Bcl-2、Bax蛋白的表达情况。结果大鼠脑组织中细胞凋亡率及Bcl-2、Bax表达水平在治疗组和模型明屁高于假手术组（P〈0．05）。在伤后72h、168h,治疗组的细胞凋亡率及Bax表达水平明显低于模型组,而Bcl-2的表达水平则明显高于模型组（P〈0．05）。结论川芎嗪可能通过抑制Bax的表达,上调Bcl-2的表达,减少神经细胞凋亡,减轻重型颅脑损伤后继发脑损害,从而发挥脑神经保护作用。     

界线类偏结核样型麻风并Ⅰ型麻风反应1例

患者男,81岁。左侧面部红肿2周。体检：左面部、左眼睑、鼻梁、眉间、额部见浸润性水肿性红斑片,界限清楚,肤温高,无压痛,皮疹表面皮肤触觉和痛觉略减弱;左眼结膜充血;余处皮肤未见红斑、色素斑和脱屑等皮疹。眶上神经、耳大神经、尺神经和腓总神经等浅表神经均未扪及粗大和压痛。皮肤组织病理检查示真皮全层和脂肪层见大量结节状分布的结核样肉芽肿改变,神经周围见淋巴细胞和组织细胞浸润。抗酸染色阳性。诊断：界线类偏结核样型麻风并Ⅰ型麻风反应。     

去细胞喉支架的制备及喉肌细胞再生的可行性研究

Objective To prepare a deeelhilarized whole laryngeal scaffold by utilizing a perfusion-decellularized technique, reseed cells on it, and construct recellularized laryngeal muscles. Methods Perfusion decelluarized larynxes were obtained by common carotid arterious perfusion with detergents. Then they were performed by macroscopic view, histological examination, scanning electron microscopy (SEM) and cartilage viability. Decellularized laryngeal scaffold were then reseeded with inducted mesenchymal stem cells (MSCs). Composites were transferred into greater omentums of rabbits after one day' s adherence and harvested after eight weeks. Macroscopic view, histological examination and immunohistochemistry were performed. Results Perfusion larynxes became transparent after two hours. Histology and SEM indicated that perfnsion method shewed better deculluarized effect. More ventages and collagen fibers but no intact cell or anclei were retained in the decellularized martrix. Porosity measured by Image pro plus 6. 0 was 80. 4% ± 3.2% (x ± s). Chondrocyte vitality assay indicated chondrocyte vitality rate in the perfusion group was 86. 9% ± 1.5% . After eight weeks, vascularization formed and integrated cartilage frameworks still remained. Histological examination could clearly show the presence of muscle bundles and vessels. Immunohistoehemical examination indicated that sarcomeric-α actin expressed positively in corresponding areas. Conclusions It is feasible to reseed MSCs into the decellularized laryngeal muscle matrix for constructing tissue-engineered laryngeal muscles. This in vivo maturation into the omentum could be the first step before in situ implantation of the construct.     

贲门失弛缓症的临床X线分析

目的 探讨食管赍门失弛缓症的X线表现及检查时的注意事项,以提高该病诊断和鉴别诊断的准确性.方法 对5例经钡餐检查和诊断以及手术治疗证实的食管贲门失弛缓症的病例进行回顾性分析.结果 5例均有特征性X线表现,温热水能适当缓解该病的梗阻症状,与临床特点相吻合.结论 根据该病的X线表现,结合食管镜等临床检查,能对该病进行准确诊断.     

以臂丛神经损害为首发表现的肺癌一例

以臂丛神经损害为首发表现的肺癌一例丁家益主治医师金建辉，沈治中，徐皖湘安徽省庐江县医院（２３１５００）肺癌首发临床表现多种多样，常见者，已为人们所熟悉，少见老，已为人们所注意，以臂丛神经损害为首发表现者，更为少见，易致误诊，近来我们遇见一例，现报告如...     

下颌骨骨折颌间固定的再评价

本文在回顾性总结下颌骨骨折200例治疗的基础上,提出对下颌骨骨折应做三维立体检查。并肯定这种概念在正确判断下颌骨骨折的移位及复位后的稳定性,指导临床医师正确选择下颌骨骨折治疗方法方面的重要意义。同时认为在合理选择适应症的条件下,用非颌间固定法治疗下颌骨骨折,可避免颌间固定的并发症又可获得满意疗效。本文还提出应用颌间固定和非颌间固定法治疗下颌骨骨折的适应症,供临床医师参考。     

成角遮线筒的自制与运用

一、原理依据光学原理有效焦点越小所得的影象越清晰,有效焦点越大投影所产生的晕影也越大。有效焦点的面积又随 X 线管方位不同而异,近阳极跟端的有效焦点最小,近阴极端的有效焦点大(见图1)     

护士心理压力的探索

目的了解影响护理人员心理健康的各种因素，探讨解除护士压力的对策。方法通过对护理人员的心理健康状态的分析与调查，针对性地进行沟通与疏导。结果根据护理人员的心理需要，应改善工作环境和条件，获得进一步学习的机会，学会自我调节，释放内心的压力，提高保护意识。结论应采取相应的措施，满足其需求，提高护士的心理健康水平。管理者要体现“以人为本”的精神，采取有效的管理对策，提高管理效能。     

将军今年九十九

今年６月３日,是张学良将军９９岁生日。这位当年发动“西安事变”的爱国名将,出生于１９００年,是本世纪的同龄人。张学良将军与赵四小姐伉俪,现居住在风光旖旎、海景秀美的夏威夷。经历了半个世纪的囚禁生涯,张将军仍然伟岸如初,身体惊人地健朗。定居夏威夷后,张...