五味子制剂治疗无黄疸型肝炎过程中出现黄疸的病例报告

病毒性肝炎目前尚缺乏特效治疗,自发现五味子有明显降低谷丙转氨酶(GPT)的作用以来,文献用以治疗病毒性肝炎的报道甚多。我院自1973年以来,亦用以治疗“难治性”无黄疸     

泛素羧基末端水解酶-1抑制剂对多巴胺能神经元的神经毒性作用

Objective To investigate the neurotoxic effects ofLDN-57444, a specific ubiquitin C-termiual hydrolase L1 (UCH-L1) inhibitor, on dopaminergic neurons and the possible mechanism. Methods The viability of SK-N-SH cells exposed to 5, 10, 25, 50, 75 or 100 μmol/L LDN-57444 for 24 h was assessed using MTT assay, and the cell apoptosis was detected with Hoechst staining. Western blot was performed to identify the expressions of UCH-L1 protein, ubiquitin monomer and polyubiquitinated proteins, and the activity of the ubiquitin-proteasome system (UPS) was evaluated with fluorometry. Results After exposure to UCH-LI inhibitor for 24 h, the cell process-like structures of SK-N-SH cells diminished, and the cell body shrank and became spherical. Exposure to LDN-57444 resulted in concentration-dependent reduction of the cell viability, and the reduction became statistically significant following the exposure to 50 μmol/L LDN-57444, as compared with that in the control group (P<0.05). The exposure also resulted in obvious cell apoptosis as shown by nuclear fragmentation and presence of the apoptotie bodies. Western blot detected no obvious changes in UCH-L1 protein expression but identified reduced ubiquitin monomer and increased polyubiquitinated protein expression in the cells. Fluorometry showed reduced activity of UPS in the exposed cells. Conclusion UCH-L1 inhibitor produces neurotoxicity to dopaminergie neurons and induces cell apoptosis possibly as the result of impaired UPS activity and intracellular accumulation of polyubiquitinated proteins following the exposure.     

岛津HD15OG—40型1250mAX线机故障检修

岛津ＨＤ１５ＯＧ—４０型１２５０ｍＡＸ线机故障检修长春市第一汽车制造厂职工医院放射线科王立非，王志全我院于８２年进口一台岛津ＨＤ１５０Ｇ－４０型Ｘ线机，配用ＶＳ－１０型遥控透视床。现将两项原因类他的故障及检修体会介绍如下：故障之一：透视时控制台上全部...     

皮肤红痣与慢性肝病之间关系的探讨

作者观察了红痣与肝病的关系。红痣系呈圆形或卵圆形,凸出皮肤,鲜红色,压之不褪,无搏动,大小、多少不一,多见于胸背部、上中腹部及上肢。观察对象包括按黄山会议标准诊断的急慢性肝炎患者112例,按1964年天津消化系统疾病学术会议拟订标准诊断的肝硬化患者19例。其红痣出现率见下表。     

卡介苗的非防痨用途

随着免疫学的发展,卡介苗(BCG)不仅作为特异性免疫预防结核病的重要手段,而且已在临床作为非特异性免疫疗法得到广泛应用。用活卡介苗作为非特异性免疫疗法,首用于治疗白血病(已有近十年历史)。随着免疫学的进展,目前认为卡介苗作为非特异性免疫疗法,仍是应用最广泛的方法之一。随着实践     

饮水型地方性砷中毒流行病学分析

山阴县是我国大陆第 3个饮水型地方性砷中毒病区 ,分布于境内黄水河流域两岸 5 km内的山阴城、黑圪塔、后所、薛×× (含省农牧场 ) 4个乡镇 ,波及 42个村庄 ,按水砷含量划分病区 ,轻病区 14村 ,中病区 12村 ,重病区 16村。病区常住人口 2 .6万 ,面积 30 0km2 。为了掌握砷中毒流行病学状况 ,分析临床特征 ,我们于 1999年 5月对居民进行了砷中毒普查。1　材料与方法　　砷中毒临床诊断按照卫生部地方病防治司颁发的《地方性砷中毒病区划分和临床诊断暂行规定》执行。对病区所有常住人口进行饮水史、患病史 ,临床表现普查 ,并对其近 5年癌症…     

磷酸化蛋白质组学揭示小热休克蛋白27是胶质细胞源性神经营养因子引起神经突触生长的新信号分子

胶质细胞源性神经营养因子(GNDF)是胶质细胞分泌的一种神经营养因子,是胶质细胞对神经元发挥保护作用最主要的途径.GNDF对多巴胺能神经元的保护及促生长作用明显,是神经保护治疗帕金森病(PD)首选神经营养因子.而对GDNF信号转导的机制尚不清楚,因此,我们用蛋白质组学的方法寻找参与GDNF信号转导通路的可能信号分子.     

西门子polymobil2型移动式X线摄影机故障检修及遥控曝光系统的设计安装

故障例1缩光器指示灯不亮。分析检修该机指示灯的光源是12V100W灯泡。工作时温度很高，经常出现插脚氧化导致接触不良。首先用锉刀和砂纸分别打磨灯脚和插孔，重新插上灯泡仍不能点亮，用万用表检查指示灯插座无电压，过热保护开关S2导通。打开机箱检查指示灯电源电路L1，测得固态继电器K1有触发信号无输出，说明K1开路。以同型号固态继电器更换，     

泛素羧基末端水解酶-1抑制剂对多巴胺能神经元的神经毒性作用

Objective To investigate the neurotoxic effects ofLDN-57444, a specific ubiquitin C-termiual hydrolase L1 (UCH-L1) inhibitor, on dopaminergic neurons and the possible mechanism. Methods The viability of SK-N-SH cells exposed to 5, 10, 25, 50, 75 or 100 μmol/L LDN-57444 for 24 h was assessed using MTT assay, and the cell apoptosis was detected with Hoechst staining. Western blot was performed to identify the expressions of UCH-L1 protein, ubiquitin monomer and polyubiquitinated proteins, and the activity of the ubiquitin-proteasome system (UPS) was evaluated with fluorometry. Results After exposure to UCH-LI inhibitor for 24 h, the cell process-like structures of SK-N-SH cells diminished, and the cell body shrank and became spherical. Exposure to LDN-57444 resulted in concentration-dependent reduction of the cell viability, and the reduction became statistically significant following the exposure to 50 μmol/L LDN-57444, as compared with that in the control group (P<0.05). The exposure also resulted in obvious cell apoptosis as shown by nuclear fragmentation and presence of the apoptotie bodies. Western blot detected no obvious changes in UCH-L1 protein expression but identified reduced ubiquitin monomer and increased polyubiquitinated protein expression in the cells. Fluorometry showed reduced activity of UPS in the exposed cells. Conclusion UCH-L1 inhibitor produces neurotoxicity to dopaminergie neurons and induces cell apoptosis possibly as the result of impaired UPS activity and intracellular accumulation of polyubiquitinated proteins following the exposure.