墨旱莲乙酸乙酯总提物对免疫抑制小鼠免疫功能的影响

目的：研究墨旱莲乙酸乙醇总提物(EAEEP)对机体免疫功能的调节作用。方法：采用腹腔注射氢化可的松或环磷酰胺所致免疫抑制小鼠模型,观察EAEEP对非特异免疫、体液免疫及细胞免疫功能的影响。结果：EAEEP能显著提高免疫抑制小鼠的免疫脏器指数、溶血素水平及外周血T淋巴细胞CD4亚群比例,并显著增强机体迟发性超敏反应。结论：EAEEP对小鼠的免疫功能有显著的调节作用。     

高效液相色谱荧光法测定Beagle犬血浆中酒石酸美托洛尔对映体浓度

目的:建立Beagle犬血浆中酒石酸美托洛尔对映体浓度的高效液相色谱荧光测定方法。方法:Beagle犬口服消旋体酒石酸美托洛尔100mg后取1·5h血样进样测定,手性色谱柱为ChiralcelOD-H,流动相为正己烷-异丙醇-二乙胺(65∶35∶0·1),荧光检测波长Ex和Em分别为267nm、290nm,柱温为25℃,流速为0·6ml/min,内标物为(S)-阿替洛尔。结果:酒石酸美托洛尔2种对映体检测浓度在10~2000ng/ml范围内线性关系良好,平均回收率为96·12%~116·9%(RSD<7·6%)。结论:该分析方法选择性好、准确性高、重现性好,适于酒石酸美托洛尔对映体的药动学研究。     

多烯紫杉醇靶向放疗增敏对结肠癌细胞凋亡及相关蛋白表达的影响

目的：探讨免疫脂质化的多烯紫杉醇在LoVo细胞放射增敏过程中， 对其凋亡及凋亡相关蛋白表达的影响。方法：制备偶联癌胚抗原（CEA）抗体的多烯紫杉醇脂质体，作用于LoVo细胞后再经2Gy照射，然后再进行相关检测：DNA末端原位标记染色法(TUNEL) 计算凋亡指数(AI);检测p53和Bax和Bcl-2，Fas，FasL及survivin 的表达。结果：多烯紫杉醇免疫脂质体联合放射组（A组）LoVo的AI为(43.6±5.2)% ，均显著高于多烯紫杉醇脂质体联合放射组（B组） (17.7±3.9)%及单纯放射组（C组） (16.8±3.5)% （P< 0. 01）。而B,C组间差异无统计学意义（P>0. 05）。A组的Bax,Fas和FasL的表达显著高于B组;而A组的survivin的表达显著低于B组（P<0.01）,但两组对p53和Bcl-2表达影响均无明显差异。结论：多烯紫杉醇经多种通路调节细胞凋亡，并能增加肿瘤细胞的放疗敏感性。     

盆底肌电刺激疗法治疗老年女性真性压力性尿失禁

目的 探讨盆底肌电刺激疗法（以下简称电刺激疗法）治疗老年妇女真性压力性尿失禁（GSI）的疗效。方法 对40例老年女性GSI患者行电刺激疗法治疗;将神经肌肉电刺激治疗仪（ETS）皮肤表面电极置于会阴部（阴道与肛门连线的两侧）。电刺激模式为专为盆底肌锻炼设计的一组程序化刺激。每周3次,每次60min,12周为1个疗程。观察治疗前后主观及客观指标变化,评估治疗效果。结果 尿失禁症状消失11例,改善21例,无改善8例。症状消失和改善者功能性膀胱容量、valsalva漏尿点压、最大尿道压和最大尿道闭合压显著高于治疗前（P〈0．05）,总排尿次数、总漏尿事件次数和ICI-Q-SF评分显著低于治疗前（P〈0．05）,总排尿量、最大尿流率、膀胱顺应性、最大膀胱压测定容量、最大尿流率时逼尿肌压力和功能性尿道长度治疗前后无明显差异（P〉0．05）。结论 神经肌肉电刺激疗法治疗老年女性GSI疗效显著．且具有无创性和经济方便等优点。     

2009年北京市呆健食品国家抽检分析及启示

2009年北京市保健食品国家抽检类别与2007年、2008年抽检类别相同,均以减肥、辅助降血糖、缓解体力疲劳类保健功能为主;抽检量达到了154件检品、覆盖90％的产品,为抽检规模最大的一次。此次抽检为更加充分全面地了解保健食品市场现状和监管提供了宝贵的基础资料。     

原发性夜遗尿症尿动力学检查评估

目的探讨原发性夜遗尿(PNE)儿童的尿动力学表现形式并评估其价值。方法156例PNE患儿分单症状性遗尿(MPE)(120例)和复杂性遗尿(CPE)(36例)二组。因上尿路疾病需要手术治疗而下尿路功能正常的20例患儿作对照组,进行膀胱压力容积、压力流率和静态尿道压力分布测定。结果MPE组中,逼尿肌不稳定收缩占56.7%(68/120)例,膀胱顺应性下降占3.3%(4/120)例,最大膀胱容量/正常膀胱容量≤80%9例;CPE组中,逼尿肌不稳定收缩占80.6%(29/36)例,膀胱顺应性降低占22.2%(8/36)例,最大膀胱容量/正常膀胱容量≤80%12例,二组比较差异有显著性意义(P<0.01)。MPE组中,尿道高压66例,逼尿肌括约肌协同失调78例;CPE组中,尿道高压25例,逼尿肌括约肌协同失调21例,二组比较差异无显著性意义(P>0.05)。MPE,CPE中逼尿肌不稳定收缩、逼尿肌括约肌协同失调和尿道压增高的发生率高于对照组,而CPE中顺应性下降的发生率显著高于对照组。结论尿动力学检查结果提示MPE、CPE二组遗尿患儿尿动力学检查的必要性。     

女性排尿功能障碍患者自由尿流率和压力-流率比较

目的：比较女性排尿功能障碍患自由尿流率和压力－流率测定的结果。方法：对50例女性排尿功能障碍患者自由尿流率测定和压力流率测定的各项指标进行比较分析。结果：发现女性排尿功能障碍患者自由尿流率测定时的最大尿流率、平均尿流率、残余尿量和排尿时间与压力流率测定时相应的指标有统计学差异（P＜0．05）；而排尿量、尿流时间和达最大尿流时间2者之间的差异无统计学意义（P＜0．05）。结论：压力流率测定的影响因素很多，应改进措施，保证其准确性，提高其临床诊断价值。     

深二度烧伤创面负压封闭引流效果观察

目的考察负压封闭引流技术（vacuum sealing drainage,VSD）治疗深二度烧伤创面的效果。方法对51例深二度烧伤患者给予分组治疗,VSD组28例,清创后应用VSD技术;常规治疗组23例,两组患者创面新鲜后移植刃厚皮片或者中厚皮片封闭创面。结果 VSD组应用5～10 d后,肉芽新鲜者去除VSD材料装置,手术移植刃厚皮片或者中厚皮片。反之,继续VSD治疗至肉芽新鲜,手术植皮。与常规换药组比较创面愈合时间缩短,疼痛减轻,费用降低。结论负压封闭引流技术对小面积二度烧伤创面愈合有明显促进作用。     

Closed reduction and intramedullary nailing versus open reduction and locking plate fixation in treatment of middle and upper humeral fractures北大核心CSCD

Objective To compare closed reduction and intramedullary nailing versus open reduction and locking plate fixation in the treatment of middle and upper humeral fractures. Methods A retrospective case-control study was conducted to analyze the clinical data of 62 patients with middle and upper humeral fracture who had been treated at Department of Orthopaedics, The First People's Hospital of Jinmen and at Department of Orthopedics, General Hospital of PLA Central Theater from October 2017 to February 2021. There were 35 males and 27 females, aged from 27 to 86 years. The left side was affected in 24 cases and the right side in 38 cases. All fractures were fresh. According to the AO classification, 16 cases were type A, 32 type B, and 14 type C. Of the patients, 29 were treated with closed reduction and intramedullary nailing (intramedullary nail group) and 33 with open reduction and locking plate fixation (locking plate group). The length of incision, operation time, intraoperative blood loss, hospital stay, fracture healing and complications were recorded and compared between the 2 groups. The pain degree was evaluated by visual analogue scale (VAS) at one week and one month after operation, and the functional recovery of the shoulder was evaluated by Constant-Murley score at one month and 12 months after operation. Results There was no significant difference in the preoperative general data between the 2 groups, showing comparability (P>0.05). The intramedullary nail group was followed up for 12 to 29 months and the locking plate group for 15 to 50 months. In the intramedullary nail group, the length of incision [(4.1±0.7) cm], operation time [(58.3±7.7) min], intraoperative blood loss [(52.7±6.5) mL], and hospital stay [(7.3±1.5) d] were significantly less than those in the locking plate group [(21.7±2.3) cm, (95.8±11.7) min, (237.4±14.9) ml, and (12.3±1.7) d] (P<0.05). The fracture healing time in the intramedullary nail group was (5.0±1.9) months, significantly longer than that in the locking plate group [(3.5±1.7) months] (P<0.05). The VAS scores at one week and one month after operation in the intramedullary nail group [(2.8±0.3) points and (1.2±0.5) points] were significantly lower than those in the locking plate group [(4.3±0.4) points and (1.6±0.5) points], and the Constant-Murley score at one month after operation in the intramedullary nail group [(63.5±7.4) points] was significantly higher than that in the locking plate group [(54.3±6.9) points] (P<0.05). However, at 12 months after operation, there was no significant difference in the Constant-Murley score between the 2 groups (P>0.05). In both groups, the VAS score at one month after operation was significantly lower than that at one week after operation while the Constant-Murley score at 12 months after operation was significantly higher than that at one week after operation (P<0.05). In the intramedullary nail group, intraoperative distal refracture happened in one case; in the locking plate group, incision infection occurred in one case and postoperative radial nerve injury in another. There was no significant difference in the incidence of complications between the 2 groups [3.4% (1/29) versus 6.1% (2/33)] (P>0.05). Conclusion In the treatment of middle and upper humeral fractures, compared with open reduction and locking plate fixation, closed reduction and intramedullary nailing shows advantages of a smaller surgical incision, shorter operation time, less intraoperative blood loss, shorter hospital stay and faster functional recovery. © The Author(s) 2022.