Relationship between hyperemesis gravidarum and Helicobacter pylori seropositivity

Early pregnancy is commonly characterized by the disturbance of digestive function,characteristically manifested by nausea and vomiting.     

体外受精-胚胎移植患者卵巢颗粒细胞卵泡刺激素受体启动子突变与卵巢反应不良的关系

Objective To sequence follicle stimulating hormone receptro (FSHR) promoter of the ovarian granulocyte and initially research the molecular mechanism of the poor ovarian response. Methods To study the relationship between FSHR promoter mutation of ovarian granulocyte and ovarian respone. The 263 bp DNA fragments before FSHR 5'initiation site in 70 cases of patients with poor ovarian respone and 88 cases of patients with ovarian normal respone who were in the cycle of IVF-ET were sequenced, Results There were 63 cases which occurred 29th site G → A point mutation in 158 women and the mutation rate was 40. 0%. Mutation rate [ 60. 0% ( 42/70 ) ] of 29th site G → A in group of poor ovarian respone was significantly higher(χ2 = 21. 450,P < 0. 01 ) than normal response group [ 23.9% ( 21/88 ) ]. There was no obviously variability ( t = 0. 457, P 0. 05 ) of basic FSH values between two groups [ G/G group was (7.2 ± 2. 3) U/L, G/A & A/A group was (7. 1±2. 0) U/L];there was obviously variability (t = 35. 81 ,P < 0. 05 ) in the number of follicles sinus between two groups ( G/G group was 14. 2±1.3, G/A & A/A group was 4. 5±0. 8 ) ;there was obviously variability ( t = 40. 35, P < 0. 05 ) in the number of ovum pick-up between two groups ( G/G group was 14. 0±1.2, G/A & A/A group was 4. 5±1.1 ) ;there was obviously variability (t =25. 80,P <0.05) of FE2-peak value between two groups [G/G group was (2 865±557) pmol/L, G/A & A/A group was (880±211 ) pmol/L] ;there was obviously variability (t =40. 22 ,P <0. 05) in the number of mature eggs ( G/G group was 13.6±1.2, G/A&A/Agroupwas4.3±0. 9).Conclusion The 29th site of FSHR promoter significantly affect the activity of FSHR promoter. Mutation of G→A can weaken promoter activity, so that ovarian granulocyte poor respone to FSH.     

端粒酶与PCNA在子宫内膜癌中表达的研究

目的探讨端粒酶与PCNA在宫内膜癌中表达临床价值。方法采用原位杂交和免疫组化方法检测内膜标本的端粒酶和PCNA的表达。结果①PCNA在正常内膜和癌组织中均存在,其表达程度与病理分级及临床分期有关。②端粒酶在正常子宫内膜和癌组织中表达率分别为10％和80．56％。差异有显著性。③PCNA的表达与端粒酶表达具有高度相关性。结论①端粒酶活化与子宫内膜癌的发生发展有密切关系,可望成为内膜癌早期临床诊断和判断预后的标志物;②端粒酶的激活导致PCNA的增殖,RCNA联合端粒酶可以判断患者的预后。     

冲击波治疗骨不连的研究

目的 通过体外冲击波疗法（ｅｘｔｒａｃｏｒｐｏｒｅａｌ ｓｈｏｃｋ ｗａｖｅ ｔｈｅｒａｐｙ,ＥＳＷＴ）治疗兔胫骨骨不连,以探求一种新的非手术治疗骨不连的方法。方法 选择健康雄性大耳白兔３０只,以改进的“切除截骨骨不连模型”制作兔右胫骨骨不连,１２周后经Ｘ光片证实为肥大型骨不连２６只,随机分为２组。治疗组１３只兔行体外冲击波骨不连断端冲击治疗,对照组不行冲击波波治疗,但均继续外固定机分为２组。治疗     

颗粒细胞卵泡刺激素受体启动子-29位点突变后的表达改变与卵巢反应不良的关系

目的 初步探讨颗粒细胞卵泡刺激素受体(FSHR)启动子-29位点突变后的表达改变与卵巢反应性的关系.方法 对体外受精-胚胎移植(IVF)周期中35例卵巢反应不良患者与15例卵巢正常反应患者FSHR 5'端起始位点的前263 bp DNA片段进行测序;收集采卵日获得的卵泡颗粒细胞, 检测FSHR的表达水平.结果 50例患者中有26例发生-29位点G→A突变, 其中4例为杂合子, 22例为纯合子, 24例测序结果 无突变, 突变的发生率为52%.-29位点基因正常组、杂合突变组及纯合突变组的FSHR表达强度依次为26.33±5.11、18.01±6.72和13.78±2.29, 三组两两比较均差异有统计学意义(P<0.01).结论 FSHR启动子的-29位点突变导致FSHR产生不足, 最终导致患者对FSH的低反应性.     

长效避孕针醋酸甲孕酮的临床效果分析

探讨长效避孕针醋酸甲孕酮（ＤＭＰＡ）的避孕效果。方法自愿使用ＤＭＰＡ避孕的育龄妇女１０３例，每３个月深部肌肉注射ＤＭＰＡ１５０ｍｇ，为期１年。结果共使用１０８９个妇女月，未采用其它避孕措施，无一例妊娠，避孕有效率１００％；１年因症停用率、累积续用率分别为１８．７５％和７８．２８％。结论ＤＭＰＡ是一种高效、简便、可接受性较高的避孕措施。     

DNA甲基转移酶抑制剂对子宫内膜异位症异位灶的影响

目的:探讨DNA甲基转移酶抑制剂5-氮-2'-脱氧胞苷对子宫内膜异位症大鼠模型异位灶的影响。方法:根据大鼠自体移植方法构建大鼠子宫内膜异位症模型。4周后二次开腹判断造模是否成功,用游标卡尺测量记录异位灶体积后将大鼠随机分为实验组(n=12)、磷酸盐缓冲液(PBS)组(n=12)和对照组(n=11),同时选取未造模的正常大鼠作为空白组(n=10)。实验组大鼠腹腔注射5-氮-2'-脱氧胞苷(2.5 mg/kg·d),PBS组注射等量PBS溶液对照组和空白组未用药。4周后取大鼠腹腔冲洗液及异位灶标本后处死大鼠,再次用游标卡尺测量备组异位灶体积大小。异位灶组织常规HE切片后显微镜下观察组织形态结构。应用免疫组织化学染色方法和Western Blot法检测备组异位灶内DNA甲基转移酶1(DNMT1)的表达情况。收集腹腔冲洗液用Elisa法检测肿瘤坏死因子(TNF-α)含量。结果:①50只大鼠根据大鼠子宫内膜自体移植方法成功35例,造模成功率为70%(35/50)。②实验组大鼠经药物处理后异位灶体积明显缩小(处理前后病灶体积分别为51.08±12.73 mm~3和5.56±3.68 mm~3),差异有统计学意义(P0.01)。③免疫组化检测实验组、PBS组、对照组异位灶组织中DNMT1表达的组织学评分分别为2.30±0.97分、3.80±1.77分、4.07±1.92分;Western Blot法检测实验组、PBS组、对照组异位灶组织中DNMT1蛋白的相对表达量分别为0.17±0.39、0.29±0.17、0.3 1±0.41。实验组异位灶组织中DNMT1表达低于PBS组及对照组差异有统计学意义(P0.05)。④实验组大鼠腹腔冲洗液中TNF-α含量较PBS组及对照组降低,实验组、PBS组、对照组均较空白组均升高差异有统计学意义(P0.05)。结论:DNA甲基转移酶抑制剂能明显减少异位灶中DNMT1的表达及腹腔冲洗液中TNF-α含量,缩小异位灶体积,促进异位灶萎缩,为子宫内膜异位症的靶向治疗提供了依据。     

联合免疫预防乙型肝炎病毒父婴传播的初步研究

研究对象来自2003年5月至2006年5月在我院产科就诊的405例孕妇,其丈夫均为HBsAg、HBeAg、抗-HBc阳性,采用完全随机实验设计方法将218例抗-HBs阳性孕妇分为A组和B组,187例抗-HBs阴性孕妇作为C组,A、C组孕妇产前肌内注射乙型肝炎免疫球蛋白。结果新生儿宫内感染率A组12．1％,B组12．8％,C组23．0％。     

体外受精-胚胎移植患者卵巢颗粒细胞卵泡刺激素受体启动子突变与卵巢反应不良的关系

Objective To sequence follicle stimulating hormone receptro (FSHR) promoter of the ovarian granulocyte and initially research the molecular mechanism of the poor ovarian response. Methods To study the relationship between FSHR promoter mutation of ovarian granulocyte and ovarian respone. The 263 bp DNA fragments before FSHR 5'initiation site in 70 cases of patients with poor ovarian respone and 88 cases of patients with ovarian normal respone who were in the cycle of IVF-ET were sequenced, Results There were 63 cases which occurred 29th site G → A point mutation in 158 women and the mutation rate was 40. 0%. Mutation rate [ 60. 0% ( 42/70 ) ] of 29th site G → A in group of poor ovarian respone was significantly higher(χ2 = 21. 450,P < 0. 01 ) than normal response group [ 23.9% ( 21/88 ) ]. There was no obviously variability ( t = 0. 457, P 0. 05 ) of basic FSH values between two groups [ G/G group was (7.2 ± 2. 3) U/L, G/A & A/A group was (7. 1±2. 0) U/L];there was obviously variability (t = 35. 81 ,P < 0. 05 ) in the number of follicles sinus between two groups ( G/G group was 14. 2±1.3, G/A & A/A group was 4. 5±0. 8 ) ;there was obviously variability ( t = 40. 35, P < 0. 05 ) in the number of ovum pick-up between two groups ( G/G group was 14. 0±1.2, G/A & A/A group was 4. 5±1.1 ) ;there was obviously variability (t =25. 80,P <0.05) of FE2-peak value between two groups [G/G group was (2 865±557) pmol/L, G/A & A/A group was (880±211 ) pmol/L] ;there was obviously variability (t =40. 22 ,P <0. 05) in the number of mature eggs ( G/G group was 13.6±1.2, G/A&A/Agroupwas4.3±0. 9).Conclusion The 29th site of FSHR promoter significantly affect the activity of FSHR promoter. Mutation of G→A can weaken promoter activity, so that ovarian granulocyte poor respone to FSH.     

体外受精-胚胎移植患者卵巢颗粒细胞卵泡刺激素受体启动子突变与卵巢反应不良的关系

Objective To sequence follicle stimulating hormone receptro (FSHR) promoter of the ovarian granulocyte and initially research the molecular mechanism of the poor ovarian response. Methods To study the relationship between FSHR promoter mutation of ovarian granulocyte and ovarian respone. The 263 bp DNA fragments before FSHR 5'initiation site in 70 cases of patients with poor ovarian respone and 88 cases of patients with ovarian normal respone who were in the cycle of IVF-ET were sequenced, Results There were 63 cases which occurred 29th site G → A point mutation in 158 women and the mutation rate was 40. 0%. Mutation rate [ 60. 0% ( 42/70 ) ] of 29th site G → A in group of poor ovarian respone was significantly higher(χ2 = 21. 450,P < 0. 01 ) than normal response group [ 23.9% ( 21/88 ) ]. There was no obviously variability ( t = 0. 457, P 0. 05 ) of basic FSH values between two groups [ G/G group was (7.2 ± 2. 3) U/L, G/A & A/A group was (7. 1±2. 0) U/L];there was obviously variability (t = 35. 81 ,P < 0. 05 ) in the number of follicles sinus between two groups ( G/G group was 14. 2±1.3, G/A & A/A group was 4. 5±0. 8 ) ;there was obviously variability ( t = 40. 35, P < 0. 05 ) in the number of ovum pick-up between two groups ( G/G group was 14. 0±1.2, G/A & A/A group was 4. 5±1.1 ) ;there was obviously variability (t =25. 80,P <0.05) of FE2-peak value between two groups [G/G group was (2 865±557) pmol/L, G/A & A/A group was (880±211 ) pmol/L] ;there was obviously variability (t =40. 22 ,P <0. 05) in the number of mature eggs ( G/G group was 13.6±1.2, G/A&A/Agroupwas4.3±0. 9).Conclusion The 29th site of FSHR promoter significantly affect the activity of FSHR promoter. Mutation of G→A can weaken promoter activity, so that ovarian granulocyte poor respone to FSH.