全文获取类型
收费全文 | 108篇 |
免费 | 6篇 |
国内免费 | 17篇 |
专业分类
耳鼻咽喉 | 3篇 |
基础医学 | 13篇 |
口腔科学 | 1篇 |
临床医学 | 16篇 |
内科学 | 6篇 |
神经病学 | 11篇 |
特种医学 | 7篇 |
外科学 | 9篇 |
综合类 | 48篇 |
预防医学 | 1篇 |
眼科学 | 1篇 |
药学 | 1篇 |
肿瘤学 | 14篇 |
出版年
2020年 | 2篇 |
2017年 | 1篇 |
2014年 | 1篇 |
2012年 | 2篇 |
2011年 | 9篇 |
2010年 | 3篇 |
2009年 | 14篇 |
2008年 | 9篇 |
2007年 | 5篇 |
2006年 | 6篇 |
2005年 | 8篇 |
2004年 | 14篇 |
2003年 | 12篇 |
2002年 | 13篇 |
2001年 | 8篇 |
2000年 | 4篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1996年 | 4篇 |
1995年 | 5篇 |
1994年 | 2篇 |
1990年 | 3篇 |
1989年 | 2篇 |
排序方式: 共有131条查询结果,搜索用时 31 毫秒
101.
102.
血管内皮生长因子受体KDR表达情况检测 总被引:1,自引:0,他引:1
目的:检测血管内皮生长因子受体KDR在不同肿瘤细胞和组织中的表达情况。方法:采用细胞酶联免疫和免疫组化法,利用抗KDR单克隆抗体,检测KDR在不同肿瘤细胞和组织表面的表达情况。结果:发现KDR在人肝癌细胞SMMC-7721和人胚胎脐静脉内皮细胞(HUVEC)表面表达阳性,在人正常细胞Wish细胞和人平滑肌细胞表面表达呈阴性。在乳腺癌组织的血管内皮细胞表达阳性,而在乳腺炎和膀胱癌组织的血管内皮细胞表达呈阴性。结论:KDR的特异性表达,可为肿瘤的靶向治疗提供一个很好的靶位点。 相似文献
103.
Objective To discuss the molecular mechanism of 18F-fluorodeoxyglucose (FDG) uptake in tumor and to assess its value to identify pathologic type and cancer staging in patients with earlystage nasopharyngeal carcinoma.Methods Forty patients with nasopharyngeal carcinoma of early-stage,including 12 cases with T1 stage and 28 cases with T2 stage, underwent FDG PET imaging.The maximum standardized uptake value ( SUVmax ) and mean standardized uptake value ( SUVmean ) of FDG uptake of each patient were measured and compared between T1 and T2 stage by t-test.The expression of glucose transport protein 1 ( Glut1 ) and hexokinase- Ⅱ ( HK- Ⅱ ) of each case was measured in paraffin sections by streptavidin-perosidase (SP) immunohistochemistry.The positive expression rate of Glut1 and HK- Ⅱ was calculated and compared between T1 and T2 by x2 test.Meanwhile, the correlation between the expression of Glut1 or HK-Ⅱ and the SUVmax was tested by Pearson analysis.Results The SUVmax and SUVmean in 40 patients were 9.45 ± 1.87 and 6.04 ± 1.09, respectively.The SUVmax of patients with T1 stage (8.95 ± 1.91 ) was significantly lower (t =4.46, P<0.001 ) than that of patients with T2 stage (11.55 ± 1.70), and the SUVmean of patients with T1 stage (5.61 ± 1.08) was significantly lower ( t = 6.76, P < 0.001 ) than that of patients with T2 stage (7.98 ± 1.10) too.Among 40 patients, all patients showed positive expression of Glut1 and HK-Ⅱ , and the positive expression rate of Glut1 and HK-Ⅱ was ( 45.2 ± 10.9 )% and ( 68.3 ±9.5)%, respectively.The positive expression rate of Glut1 was (38.4 ±8.1)% in T1 stage and (49.7 ±12.6)% in T2 stage, which displayed no difference (x2 =40.58, P>0.05), but the HK-Ⅱ positive expression rate showed significant difference (x2 =58.71, P<0.05) between T1 stage (60.1 ±11.1)% and T2 stage (77.9 ± 14.7 )%.The correlation analysis indicated that there was low-degree positive correlation (r =0.369, P=0.019) between the SUVmax and Glut1 expression, and there was medium-degree positive correlation (r = 0.549, P = 0.001 ) between the SUVmax and HK-Ⅱ expression.Conclusion Expression of Glut1 and HK-Ⅱ was positively correlated with FDG uptake in patients with early-stage nasopharyngeal carcinoma. 相似文献
104.
目的 研究早期鼻咽癌组织血管内皮细胞生长因子(VEGF)表达及肿瘤组织~(18)F-FDG摄取的情况,并探讨早期鼻咽癌组织VEGF表达与~(18)F-FDG摄取的关系.方法 对40例I,II期鼻咽癌患者进行正电子发射体层显像检查,测定鼻咽原发灶肿瘤组织的~(18)F-FDG最大摄取值和标准摄取值(SUV_(max)和SUV_(mean)),应用标准链霉菌抗生物素蛋白-过氧化物酶亲和免疫组化法检测40例患者肿瘤组织VEGF的表达.结果 40例早期鼻咽癌组织的SUV_(max)>与SUV_(mean)分别为9.45±1.87和6.04±1.09,T_1期肿瘤原发灶SUV_(max)与SUV_(mean)分别为8.95±1.91和5.61±1.08,T_2期的原发灶SUV_(max)与SUV_(mean)分别为11.55±1.70和7.98±1.1,T_2期原发灶SUV_(max)和SUV_(mean)高于T_1期(t=4.46,P<0.001;t=6.763,P<0.001).非角化分化型肿瘤原发灶SUV_(max)与SUV_(mean)分别为9.74±1.82和6.82±1.23,非角化未分化型肿瘤原发灶SUV_(max)与SUV_(mean)分别为10.44±2.16和6.68±1.35,非角化分化型和非角化未分化型肿瘤原发灶SUV_(max)和SUV_(mean)无差别(t=1.230,P>0.05;t=0.346,P>0.05).40例鼻明癌组织VEGF染色阳性细胞率为60.8%;鼻咽癌组织FDG摄取(SUV_(max))和VEGF表达的细胞阳性率相关(r=0.460,P=0.03).结论 早期鼻咽癌组织FDG摄取与肿瘤组织VEGF过度表达相关,其SUV值反映了鼻咽癌组织的葡萄糖代谢情况,同时也在一定程度上反映了肿瘤组织乏氧的程度.Abstract: Objective To study the overexpression of vascular endothelial growth factor (VEGF) and fluorine-18 fluorodeoxyglucose (FDG) uptake in early-stage nasopharyngeal carcinoma (NPC) and evaluate their relationship. Methods FDG positron emission tomography (PET) was performed in forty patients with stage I and stage II NPC. The maximum and mean standard uptake values (SUV_(max) and SUV_(mean) ,respectively) were measured in each patient, and the expression of VEGF was measured on paraffin sections using immunohistochemistry. Results The FDG uptake in the patients were 9.45±1.87 (SUV_(max)) and 6.04±1.09 (SUV_(mean)), 8.95±1.91 (SUV_(max)) and 6.04±1.09 (SUV_(mean)) in stage I patients, and 11.55±1.70 (SUV_(max)) and 7.98±1.1 (SUV_(mean)) in stage II patients. The FDG uptake of stage II patients was higher than that of stage I patients. The FDG uptake of non-keratinizing differentiated carcinoma was 9.74±1.82 (SUV_(max)) and 6.82±1.23 (SUV_(mean)) and 10.44±2.16 (SUV_(max)) and 6.68±1.35 (SUV_(mean)) in non-keratinizing undifferentiated carcinoma, showing no significant differences between them (SUV_(max): t=1.230, P>0.05; SUV_(mean): t=0.346, P>0.05). The VEGF-positive cells were 60.80% in the tumor. A correlation between VEGF expression and FDG uptake in he tumor was noted (r=0.460, P=0.03). Conclusion VEGF overexpression is correlated to FDG uptake in patients with early-stage NPC. The SUV value reflects the glucose metabolism of NPC, and also shows the degree of oxygen insufficiency in the tumor tissue. 相似文献
105.
目的 测定并分析早期鼻咽癌18F-脱氧葡萄糖(FDG)摄取与肿瘤组织葡萄糖转运蛋白1(Glut1)及己糖激酶-Ⅱ(HK-Ⅱ)的表达,以探讨鼻咽癌组织摄取FDG的分子机制及其与肿瘤病理类型和分期的相关性.方法 40例早期鼻咽癌患者(T1期12例,T2期28例)行PET检查,测定病灶最大标准摄取值(SUVmax)及平均标准摄取值(SUVmean) 行鼻咽部肿瘤活组织检查,并采用免疫组织化学法测定其Glut1及HK-Ⅱ表达,计算阳性细胞百分率.T1与T2期患者SUVmax或SUVmean间的差异采用t检验,Glut1及HK-Ⅱ表达阳性率差异采用x2检验,SUVmax与Glut1和HK-Ⅱ表达间的关系采用Pearson相关分析.结果 40例患者SUVmax与SUVmean分别为9.45±1.87和6.04±1.09.T1期分别为8.95±1.91和5.61±1.08,T2期分别为11.55±1.70和7.98±1.10,T1期与T2期相比原发灶SUVmax(t=4.46,P<0.001)及SUVmean(t=6.76,P<0.001)差异均有统计学意义.40例早期鼻咽癌患者Glut1及HK-Ⅱ染色均为阳性,阳性细胞率分别为(45.2±10.9)%与(68.3±9.5)%.其中Glut1阳性细胞率在T1期和T2期分别为(38.4±8.1)%与(49.7±12.6)%,两者差异无统计学意义(x2=40.58,P>0.05),而HK-Ⅱ阳性细胞率在T1期与T2期分别为(60.1±11.1)%与(77.9±14.7)%,差异有统计学意义(x2=58.71,P<0.05)).SUVmax与Glut1表达阳性细胞率呈低度正相关(r=0.369,P=0.019),与HK-Ⅱ表达阳性细胞率呈中度正相关(r=0.549,P=0.001).结论 早期鼻咽癌组织FDG摄取与Glut1过度表达相关. 相似文献
106.
107.
108.
109.
目的 探讨血管成形术后Ⅰ型胶原的动态演变规律及其与MMP 1和TIMP 1的关系。方法 采用小型猪髂动脉再狭窄动物模型 ,分别在血管成形术后 1、2、3和 6个月取材 ,用免疫组化、透射电镜加图象分析技术观察血管内膜增生、平滑肌细胞 (VSMC)表型特征、Ⅰ型胶原、MMP 1和TIMP 1的表达情况。结果 血管内膜增生在成形术后 3个月达高峰 ,Ⅰ型胶原的表达水平在成形术后逐渐增高 ,MMP 1的表达水平在血管成形术后早期较低 ,至 6个月时达正常血管水平 ,而TIMP 1在血管成形术后早期即明显增高 ,在 3个月内达高峰 ,6个月时仍稳定在较高水平 ;VSMC表型在早期主要是增生型 ,而后期以收缩型为主 ;TIMP 1/MMP 1的比值与新生内膜面积及Ⅰ型胶原的表达水平均呈显著正相关 (r=0 6 5 ,P <0 0 1)。结论 血管成形术后细胞外Ⅰ型胶原随着时间的推移而逐渐增加 ,Ⅰ型胶原的动态演变主要取决于TIMP 1/MMP 1的活性比值并与VSMC表型特征有一定的联系。 相似文献
110.
重组人生长激素对中性粒细胞凋亡和存活的影响 总被引:2,自引:1,他引:1
目的:研究重组人生长激素(rhG)对有(或)无H LPS刺激的离体培养中性粒细胞(PM N s)凋亡和存活的影响。方法:采健康人外周静脉血,血浆-Percoll不连续密度梯度离心法分离出PM N s,分为4组:对照组,rhG H(100ng/m L)组,LPS100ng/m L组,LPS100ng/m L刺激1h后加rhG H100ng/m L组,离体培养3h和24h上流式细胞仪检测PM N s的凋亡(A nnexin V法)。结果:无LPS刺激状态下,rhG H可引起PM N s早期凋亡率减少、存活率增加,但不影响晚期凋亡/死亡率;有LPS刺激状态下,rhG H不影响PM N s早期凋亡率、晚期凋亡/死亡率和存活率,而且可能对LPS对PM N s刺激有一定阻抑作用。结论:无LPS刺激状态下,rhG H可延迟PM N s凋亡、增强PM N s活力;与LPS共同孵育时,rhG H不影响PM N s凋亡和活力,且对LPS对PM N s的刺激有一定阻抑的作用。 相似文献