胰腺B细胞显像剂~(99)Tc~m-DTPA-NGN2在小鼠体内的分布

目的 研究胰腺B细胞显像~(99)Tc~m-DTPA-NGN2在小鼠体内的分布规律,为~(99)Tc~m-DTPA-NGN2用于临床胰腺B细胞显像及胰腺癌的核医学显像做基础.方法 正常小鼠42只,分为7组,每组6只,尾静脉注射~(99)Tc~m-DT.PA-NGN2 3.7 MBq后5、15、30 min和1、2、4、6 h放血处死,取心、肝、脾、肺、脑、肾、肌肉、骨、小肠、胃、胰腺和血液等组织或器官,称重并测量其放射性,计算每克组织百分注射剂量率(%ID/g).同时,取正常小鼠5只,尾静脉注射~(99)Tc~m-DTPA-NGN218.5 MBq后相同时间进行显像.结果 ~(99)Tc~m-DTPA-NGN2 主要经肾脏代谢,脑内未见摄取,各组织、器官的百分注射剂量率在1 h内均迅速下降,胰腺的百分注射剂量率随时间的延长逐渐上升,在1～2 h内保持较高水平.显像结果与生物分布实验结果一致,随时间的延长胰腺显影逐渐清晰,在1～2 h内均可清晰观察胰腺组织.结论 ~(99)Tc~m-DTPA-NGN2可能是一种较好的胰腺B细胞显像剂,在胰腺B细胞的功能显像和胰腺癌显像的应用中值得进一步研究.

Abstract：

Objective To evaluate the biodistribution and imaging of technetium-99m labeled Nateglinide: ~(99)Tc~m-DTPA-Nateglinide (~(99)Tc~m-DTPA-NGN2) in mice. Methods Forty-two healthy Kunming mice were divided into 7 groups. Five minutes, 15 minutes, 30 minutes, 1 hour, 2 hours, 4 hours,and 6 hours after the injection of 3.7 MBq ~(99)Tc~m-DTPA-NGN2, these mice were sacrificed by cutting the carotid. Selected tissues (heart, liver, spleen, lung, brain, kidney, muscle, bone, intestine, stomach, pancreas and blood) were excised and weighed, and the radioactivity was measured. The biodistribution of radiotracer in each tissue sample was calculated as a percentage of the injected dose per gram of wet tissue weight (%ID/g). Another 5 mice were imaged after the injection of 18.5 MBq ~(99)Tc~m-DTPA-NGN2 at the same time. Result ~(99) Tc~m-DTPA-NGN2 is metabolized mainly from kidney, brain doesn't uptake ~(99) Tc~m-DTPA-NGN2, the %ID/g of the selected tissues were decreased rapidly in 1 hour, and the radioactivity in pancreas kept stable from 1 hour to 2 hours after injection. The pancreas visualized clearly 1 hour after injection. Conclusion ~(99) Tc~m-DTPA-NGN2 can be clearly visualized in pancreas in mice, and maybe it's promising in imaging pancreatic tumor and in evaluating the function of pancreatic B cell.     

基于多学科协作的综合护理对肺结核合并2型糖尿病患者自我管理的影响

目的:探讨基于多学科协作的综合护理对肺结核合并2型糖尿病患者自我管理的影响。方法:选择本院2021年1月—2022年12月收治的108例肺结核合并2型糖尿病患者为研究对象,按奇数和偶数分组,分为观察组和对照组,各54例。对照组给予常规护理,观察组在其基础上给予基于多学科协作的综合护理,比较两组干预效果。结果:干预后观察组患者的负面情绪评分低于对照组(P<0.05);干预后观察组患者的自我管理能力明显高于对照组(P<0.05);干预后观察组患者的满意度明显更高(P<0.05)。结论:基于多学科协作的综合护理对肺结核合并2型糖尿病患者的应用效果十分显著,有效提高患者自我管理能力,疏导负面情绪,减缓病情恶化,具有很高的优势,值得应用和推广。     

汕头市耐多药肺结核患者二线药敏试验结果分析

目的分析汕头市住院耐多药肺结核患者结核分枝杆菌对二线抗结核药物的耐药情况,为制定治疗方案提供依据。方法分析2010年8月至2012年7月135例住院耐多药肺结核患者结核分枝杆菌对Am、Lfx和Pro的药物敏感性试验结果,观察初治组和复治组对二线抗结核药物的耐药率。结果初治组耐Lfx10．0％、耐Am10．O％、耐Pr06．7％,复治组耐Lfx23．8％、耐Am17．1％、耐Pt04．8％;初治组未发现广泛耐药患者,复治组中广泛耐药患者13例（12．4％）。结论对于耐多药肺结核患者,进行二线抗结核药物敏感试验,有利于耐多药肺结核个体化方案的制定。