宫瘤消胶囊的质量标准研究

目的 建立宫瘤消胶囊的质量标准,为完善其质量标准提供科学依据。方法 采用TLC鉴别方中三棱、三七、沉香及丹参;采用HPLC对方中丹酚酸B的含量进行测定。结果 TLC中可检出三棱、三七、沉香与丹参的特征斑点,阴性对照无干扰;丹酚酸B在0.202 2~2.022 0μg具有良好的线性关系,平均回收率为96.83%,RSD为1.69%（n=6）。结论 该方法能准确可靠的进行定性、定量检测、重复性好,可作为宫瘤消胶囊的质量控制标准。     

改良Devine术与传统Shiraki治疗小儿隐匿性阴茎的临床探讨

目的：对小儿隐匿性阴茎患者进行改良Devine术与传统Shiraki术,做好临床效果的探究。方法：选取2020年1月至2021年1月本院收治的隐匿性阴茎患儿60例,采用随机分组方式,每组人数为30例。常规组采用Shiraki手术治疗,试验组采用改良Devine手术治疗,做好治疗效果、术后阴茎延长情况比较、并发症效果的比较。结果：试验组患者临床疗效比常规组显著要更好（P<0.05）,试验组采用治疗中有效、显效、无效人数分别为21例、7例、2例,.所占比例分别为93.3%。试验组患者中术前阴茎长度、术后阴茎长度分别为（1.78±0.63）cm、（4.47±0.57）cm,常规组患者中术前阴茎长度、术后阴茎长度分别为（1.85±0.27）cm、（2.92±0.10）cm,经比较,术后情况明显改善,两组患者具有一定差异（P<0.05）。通过对患者进行并发症情况比较,其中常规组患者出现术后包皮严重水肿、阴茎回缩人数分别为6例、1例,试验组患者人数分别为1例、0例,经比较,试验组效果较优（P<0.05）。试验组治疗后生活质量整体效果较好（P<0.05）。治疗后试验组的生活质...     

广西新生儿遗传性非综合征型耳聋基因筛查

目的 应用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术检测广西新生儿遗传性非综合征型耳聋(NSHI)基因突变基因,探讨耳聋基因筛查在临床中应用的有效性和可行性.方法 对7 100例出生的新生儿采用自动判别听性脑干诱发电位(AABR)进行听力初筛和复筛,并通过足跟血斑提取基因组DNA,采用MALDPTOF-MS进行4个耳聋易感基因20个突变住点检测.结果 7 100例新生儿听力初筛通过率为97.11％(6 895/7 100),新生儿基因突变阳性率为3.54％(251/7 100),其中GJB2基因突变131例,携带率为1.84％,235delC杂合突变108例.SLC26 A4基因突变93例,以1229C＞T杂合突变和IVS7-2A＞G杂合突变为主,mtDNA12SRNA基因突变16例,GJB3基因突变11例.结论 采用MALDI-TOF-MS技术筛查可提高常见耳聋相关基因热点突变检出率,从分子水平发现新生儿遗传性NSHI,可为早期发现、预测耳聋的发生及制订干预措施提供相应的遗传咨询指导.     

肾病综合征的中医药治疗近况

肾病综合征(Nephrotic syndrome,NS)是指多种肾脏病理损害所致的一组临床症侯群。其表现为重度蛋白尿、低蛋白血症、高脂血症、水肿等。目前对本病治疗多采用糖皮质激素、免疫抑制剂等,虽有一定疗效,但长期服用有较多不良反应,且停药后易复发。因此,寻求防治肾病综合征的有效措施和改善肾功能的药物疗法,是肾病内科领域中一项十分重要的课题。近年来大量临床研究表明中医药对肾病综合征取得了较好的疗效,现综述如下。     

地中海贫血基因携带合并感染人细小病毒B19孕妇的免疫相关研究

<正>人细小病毒(HPV)B19属于微小病毒族、红细胞病毒属,是目前动物病毒中体积最小、最简单的一类线状单链DNA病毒。是由非结构蛋白NS1和结构蛋白VP组成,其细胞毒性来自非结构蛋白的表达,在成红血细胞核内繁殖,通过侵袭使骨髓内红系造血细胞在成熟最后阶段被破坏,溶解成红细胞,导致溶血和红细胞生成障碍[1]。而地中海贫血(简称地贫)是由于珠蛋白基因发生缺陷,致使珠蛋白链合成减少或缺如,使形成血红蛋白的α链/非α链比例     

猪胰岛细胞异种移植激活立即经血液介导炎症反应的体外模型构建

BACKGROUND: Islets rapid loss during the early stage of transplantation is caused by immediately blood-mediated inflammatory reaction (IBMIR). It is necessary to establish a stable and standard IBMIR model in vitro to study the mechanism and therapy methods of IBMIR. OBJECTIVE: To observe the performances of IBMIR in vitro induced by adult porcine islets perfusion with fresh human blood, and establish a stable IBMIR model in vitro with heterogenous islets. DESIGN, TIME AND SETTING: An in vitro experiment based on observation was performed at the Cell Transplantation and Gene Therapy Institute of the Third Xiangya Hospital of Central South University from January to November 2008. MATERIALS: Fresh human blood was obtained from healthy staff of the Cell Transplantation & Gene Therapy Institute, divided into blank, control, and experimental groups. The donor pigs, Hunan shaziling pigs, were provided by the Animal Department of Xiangya Medical College, Central South University. METHODS: Adult porcine islets (APIS) were digested and collected using enzyme digestion and density gradient centrifugation. APIS of 5 000 and 10 000 islet equivalent (IEQ) were perfused with human blood in heparinized PVC tubing loops (ultimate heparin concentrations in blood were 0.001, 0.01, 0.02 g/L respectively). The loops were placed on the shaking device in the incubator for 5, 10, and 60 minutes to simulate blood flow in vessels. The fresh blood of blank group was detected immediately after extraction; 7 mL fresh blood of control group was mixed with 100 µL culture solution to shock for 60 minutes. MAIN OUTCOME MEASURES: To analyze the blood platelet counts, different leukocyte counts, the levels of C3a, C5b-9, thrombin-antithrombine complex (TAT) and β-thromboglobulin (TG). Macroscopic blood clots were collected from the loops for Hematoxylin-eosin staining analysis. RESULTS: 0.001 g/L heparin in Tubing Loops exhibited minimal influence on physiological function of fresh blood, which could simulate in vivo blood physiological function over a long period of time. When adult porcine islets were perfused with human blood, blood clot appeared in 10 000 IEQ group within 10 minutes, and increased with time; the number of platelet in Tubing Loops decreased with time, while there was a pronounced activation of the coagulation, complement and leukocyte inflammatory systems. Those changes were significant compared with 5 000 IEQ group. Blood clot pathological detection shows that islet cells were surrounded by thrombus, and infiltrated with leukocytes. The capsule of islet transplant was destroyed and split. CONCLUSION: 10 000 IEQ adult porcine islets added into the heparinized tubing loops with 0.001 g/L heparin successfully established a stable and standard IBMIR model in vitro.     

颅内血肿微创穿刺粉碎清除术治疗急性重型脑出血的临床研究

我院自2002～2003年12月采用YL-1型颅内血肿微创穿刺粉碎清除术治疗31例急性重型脑出血患者，取得满意疗效，现报道如下。     

复肾口服液对阿霉素肾病大鼠肾组织及血清MDA的影响

目的:探讨复肾口服液对肾病综合征的治疗作用。方法:雄性Wistar大鼠一次性尾静脉注射阿霉素建立肾病模型,造模后7天开始灌胃给药,连续28天。观察该药对大鼠肾组织及血清脂质过氧化产物丙二醛(MDA)含量的影响。结果:盐酸阿霉素尾静脉注射后7天,模型组大鼠24h尿蛋白定量较正常组明显增加,肾组织及血清MDA含量明显高于正常对照组。与模型组比较,复肾口服液可显著减少尿蛋白的排出,降低肾组织及血清MDA含量,抑制肾组织的过氧化损伤。结论:复肾口服液可以通过降低大鼠肾组织及血清MDA,起到对肾病综合征大量蛋白尿的治疗作用,但存在着剂量依赖关系。     

消融阻隔气囊对胃肠保护作用的实验研究

[目的]探讨家猪射频消融临近胃肠肝组织时,消融阻隔气囊能否有效保护邻近胃肠.[方法]在5只猪全麻后进行CT引导下消融阻隔气囊在射频消融治疗保护作用的随机对照实验.每支猪治疗两个位点,实验组加用消融阻隔气囊保护,对照组不加用阻隔气囊,术后3～4 d处死猪,观察并测量坏死组织面积.观察组织坏死病理变化.[结果]实验组和对照组分别5个位点,实验组消融时间为(16±2.6)min,肝组织坏死最大直径为(4.55±0.50)cm;对照组消融时间为(16±1.8)min,肝组织坏死最大直径为(4.67±0.60)cm,t检验两组之间治疗时间和肝组织坏死最大直径没有显著性区别.实验组无临近胃肠损伤.对照组胃肠均有热损伤,胃肠损伤直径平均3.5 cm,未见明显穿孔.[结论]消融阻隔气囊可以有效保护射频消融时邻近器官,减少热损伤并发症.