The effects of β-NGF controlled delivery system on growth of primary cultured chick embryo dorsal root ganglion axons

Objective To construct β-NGF controlled delivery system and evaluate the biological effects of β-NGF on the growth of chick embryo dorsal root ganglion (DRG) axons in vitro. Methods Delivery systems releasing β-NGF at 50/μL, 100/μg/L and 250 μg/L concentration were constructed. To determine the optimal dose response effects of NGF in the controlled delivery system, DRG were co-cultured with of β-NGF at above concentrations while using DRG basic culture as control. Axonal growth was observed. DRG were also cocultured with the components in the controlled delivery system to detect the effects on growth of DRG axons. The experiment was divided into 5 experimental groups and 1 control group: control group, DRG+ fibrin; Group A,DRG+ fibrin+ peptide + heparin + 100 μg/L β-NGF; Group B, DRG + fibrin + heparin + 100/μg/L β-NGF;Group C, DRG + fibrin + peptide + 100 μg/L β-NGF; Group D, DRG + fibrin + 100 μg/L β-NGF; Group E,DRG + fibrin + peptide + heparin. Results The growth of DRG axons in 50 μg/L, 100μg/L and 250/μg/Lconcentration of β-NGF controlled delivery system was 1.31 ( P > 0. 05), 3.78 ( P < 0. 01 ) and 3.05 ( P <0.01) folds of the control respectively. The growth of DRG axons in 100 μg/L group was significantly better comparing to that in 250 μg/L group. The growth of DRG axons in Groups A, B, C, D and E was 3.75, 1.15,1.12, 1.10 and 1.09 folds of the control group, respectively. The difference was only statistically significant between Group A and the control group ( P < 0. 01 ). Conclusion β-NGF released from the β-NGF controlled delivery system was bioactive. It could promote the growth of DRG axons.     

TASC-D型髂动脉病变的腔内治疗

目的 探讨对泛大西洋协作组织(TransAtlantic Inter-Society Consensus Working Group,TASC)-D型髂动脉闭塞性病变腔内治疗的疗效.方法 回顾性分析自2006年6月至2010年6月,TASC-D型髂动脉病变腔内治疗的26例患者(共31条肢体)的临床资料.单纯髂动脉病变10例,髂动脉合并腹股沟以下病变16例.结果 24例患者(28条肢体)获得成功,技术成功率90.3%(28条/31条),成功的28条肢体临床症状改善率100%(28条/28条),治疗成功者共放置支架44枚,其中8条肢体进行腹股沟以下动脉腔内治疗,3条肢体同时行股-腘人工血管旁路术,8例患者辅助超声消融技术.术后踝-肱指数(ankle-brachial index,ABI)为0.67±0.16,较术前0.37 ±0.15提高,两者差异有统计学意义(t=13.24,P<0.01).随访22例(26条肢体),时间3～44个月,平均(22±11)个月.1年初期通畅率90%(18/20);二期累积通畅率95%(19/20);3年初期通畅率70%(7/10),二期累积通畅率80%(8/10).结论 对于难以耐受外科手术的TASC-D型髂动脉病变可以选用腔内治疗.

Abstract：

Objective To evaluate recanalization for TASC-D type iliac artery lesions.Methods Between June 2006 and June 2010,26 patients with a total of 31 limbs of the TASC-D iliac artery lesions underwent endovascular procedure. Results Technical success rate was 90.3% (28/31).Clinical symptom improvement rate was 100%. Forty-four stents were placed in 28 limbs of the 24 patients.Eleven patients underwent an associated procedure; femoropopliteal endovascular procedures in 8 cases,femoro-popliteal bypass in 3 cases. Eight patients underwent an intravascular ultrasound thrombolysis.Postoperative ankle-brachial index ( ABI) was increased by more than 0. 15 in all patients. Twenty-two patients(26 limbs) were followed-up for 3 -44 mos, the primary patency rate at 12 months was 90% ,and cumulative patency rate was 95%. At 36 months primary patency rate was 70% , and secondary patency rate was 80%. Conclusions Because the technical success rate is high, endovascular techeniques are the choice of therapy in high risk patients with TASC-D type iliac artery lesions.     

锥形Amphirion Deep长球囊治疗膝下动脉缺血15例分析

应用Amphirion Deep球囊治疗下肢动脉缺血在国外已广泛开展,在国内发展也很迅速,其确切疗效不断被证实[1],但应用锥形Amphirion Deep长球囊治疗膝下动脉缺血迄今未见报道.我院自2009年3月至2009年9月应用锥形Amphirion Deep长球囊血管成形治疗膝下动脉缺血共15例(15条肢体),现报告如下.     

48例高血压脑出血术后再出血临床分析

我科自2000年8月至2006年4月共施行高血压脑出血手术497例，术后发生再出血48例，发生率为9．66％。现就其再出血危险因素及预防措施分析如下。     

电解可脱卸弹簧圈栓塞治疗颅内动脉瘤22例报告

目的探讨电解可脱卸弹簧圈(GDC)血管内栓塞治疗颅内动脉瘤的临床效果和应用价值。方法应用GDC治疗破裂动脉瘤22例,动脉瘤25个。结果术后随访6个月~2年均无复发,无再次蛛网膜下腔出血。2例术后1~3个月出现脑积水,行脑室腹腔分流术,术后均恢复良好。结论GDC血管内栓塞治疗颅内动脉瘤是一种安全、有效、可靠的治疗方法。     

脑室-腹腔分流管腹腔端外露1例报告

1病例介绍患者男性,62岁,因“脑室-腹腔分流术后3年,发现腹部异物半天”步行入院,CT提示：脑室系统扩张,见分流管脑室端位于右侧脑室内。腹部X线：可见分流管位于腹部。查体：精神亢奋,对答错误,记忆力、计算力、定向力差,腹平软,无肌紧张,无压痛以及反跳痛,分流管路径体表皮肤无红肿、破溃,右下腹可见一约6cm陈旧性手术疤痕,脐下正中约3cm处可见一皮肤潮红窦道,长约3cm,分流管从窦道穿出,压迫分流泵,分流管腹腔端可见清亮脑脊液流出（见附图）。     

立体定向显微手术治疗大脑半球运动区病灶

1对象与方法我科于2003年5月～2005年12月．对20例大脑半球运动区病灶应用立体定向脑沟入路开颅病灶切除术，其中男11例，女9例；年龄15～69岁，平均41岁。临床表现为癫痫大发作8例．部分性发作12例。病灶直径10～20mm 4例．21～30mm／113例，〉30mm／13例。病灶均位于皮质下．硬膜切开后肉眼均不能识别。     

气源质量--气动阀门寿命的保障

气动阀门的气缸和定位器，其可靠性和寿命是衡量产品质量的主要指标，然而现场往往是由于气源质量不好而造成故障，这已成为某些工厂的一大问题。本文对此作了分析举例，文后对气源处理水平的提高作了一些建议。     

泌尿系结石腔镜手术中肾盂内压力的研究进展

泌尿系结石是泌尿系统常见的疾病之一,大部分为上尿路结石。随着泌尿系结石微创技术的不断推广,输尿管镜碎石术及经皮肾镜碎石术等泌尿腔镜手术已广泛应用于上尿路结石的治疗中。上尿路结石腔镜手术中容易引起肾盂内压力(renal pelvic pressure,RPP)的升高,增加术后并发症的发生,造成肾脏一定的损害,甚至导致致死性尿源性脓毒症及感染性休克,严重威胁患者的生命。本文旨在对泌尿系结石腔镜手术中RPP的相关研究做一综述。     

急性视网膜坏死综合征的临床治疗

目的 探讨急性视网膜坏死综合征临床治疗方法。方法 回顾性分析3例（3眼）急性视网膜坏死综合征的临床治疗情况。结果 1例视力明显下降4d后确诊，全身和局部用阿昔洛韦抗病毒；复方托品酰胺散瞳，甲基强的松龙点眼，口服强的松和阿司匹林抗炎；静点葛根素、口服丹参滴丸、怡开等维持视网膜循环通畅；口服弥可保、肌苷、维康福、维生素C、E支持营养视网膜；经过2周治疗最佳校正视力由原来2尺指数恢复到0．4，经过9个月治愈，再过1年多复查，仅遗留少许玻璃体浑浊和周边视网膜色素沉着，最佳校正视力恢复到0．8。另1例视力明显下降1个月被确诊，给以利巴韦林抗病毒，复方托品酰胺散瞳，进行玻璃体切割联合眼内视网膜光凝术，术前视力0.3，术后1周出院，见视网膜在位，视力0．1，半年后复查视力仍未提高。再1例视力明显下降1周被确诊，当时视力0．1，给以阿昔洛韦抗病毒，复方托品酰胺散瞳，口服强的松和阿司匹林抗炎，因为发现周边视网膜裂孔，进行视网膜光凝术，治疗2周后出院，视力0．2。结论 及时诊断，积极治疗是急性视网膜坏死综合征治愈、获得优良视功能的关键。我们的治疗提示：综合运用有效抗病毒、疏通视网膜循环、糖皮质激素和非甾体类激素、支持营养视网膜是有效的治疗方法；应慎重进行玻璃体切割术和视网膜光凝术，掌握好手术时机。