缝隙连接细胞间通讯在炎症、损伤修复和疾病中的作用

缝隙连接是相邻细胞间的通道结构,其主要功能是细胞通讯,又称缝隙连接细胞间通讯。缝隙连接细胞间通讯是细胞间最重要的信息交流形式,可调节组织细胞的生长、分化,在组织保持内稳态中处于核心地位。本文就缝隙连接细胞间通讯在炎症、损伤修复和疾病中的作用等方面作一综述。     

缝隙连接蛋白的非通讯功能

缝隙连接是相邻细胞间通道结构,其"看家功能"是细胞通讯,又称缝隙连接细胞间通讯参与许多重要生物学过程的调节.近年大量研究证实,缝隙连接蛋白作为一个单独的功能单位,可调节细胞的生长、增殖、分化、凋亡等生物学过程.本文对缝隙连接蛋白上述功能作一综述,并探讨其可能机制.     

急诊后颅窝开颅术后并发颅内感染27例临床分析

后颅窝手术后感染是临床上较为常见的并发症,文献报道5%～13.4%,为幕上开颅的3倍[1]。我科自1998-01～2010-03共行急诊后颅窝开颅手术157例,并发颅内感染27例(17.20%),与择期后颅窝开颅术后并发颅内感染相比明显增高,现予以报告,并结合文献加以讨论。1临床资料     

脑动静脉畸形手术治疗体会

目的讨论颅脑动静脉畸形的临床特征和临床治疗方法,以及治疗新进展。方法分析该病的临床表现、影像学特征、探讨其治疗方法,通过两例典型的病例进行讨论。结果手术全切除59例,占92％,部分切除5例,占8％。结论手术治疗是目前治疗脑动静脉畸形并出血的有效方法。     

锥形Amphirion Deep长球囊治疗膝下动脉缺血15例分析

应用Amphirion Deep球囊治疗下肢动脉缺血在国外已广泛开展,在国内发展也很迅速,其确切疗效不断被证实[1],但应用锥形Amphirion Deep长球囊治疗膝下动脉缺血迄今未见报道.我院自2009年3月至2009年9月应用锥形Amphirion Deep长球囊血管成形治疗膝下动脉缺血共15例(15条肢体),现报告如下.     

TASC-D型髂动脉病变的腔内治疗

目的 探讨对泛大西洋协作组织(TransAtlantic Inter-Society Consensus Working Group,TASC)-D型髂动脉闭塞性病变腔内治疗的疗效.方法 回顾性分析自2006年6月至2010年6月,TASC-D型髂动脉病变腔内治疗的26例患者(共31条肢体)的临床资料.单纯髂动脉病变10例,髂动脉合并腹股沟以下病变16例.结果 24例患者(28条肢体)获得成功,技术成功率90.3%(28条/31条),成功的28条肢体临床症状改善率100%(28条/28条),治疗成功者共放置支架44枚,其中8条肢体进行腹股沟以下动脉腔内治疗,3条肢体同时行股-腘人工血管旁路术,8例患者辅助超声消融技术.术后踝-肱指数(ankle-brachial index,ABI)为0.67±0.16,较术前0.37 ±0.15提高,两者差异有统计学意义(t=13.24,P<0.01).随访22例(26条肢体),时间3～44个月,平均(22±11)个月.1年初期通畅率90%(18/20);二期累积通畅率95%(19/20);3年初期通畅率70%(7/10),二期累积通畅率80%(8/10).结论 对于难以耐受外科手术的TASC-D型髂动脉病变可以选用腔内治疗.

Abstract：

Objective To evaluate recanalization for TASC-D type iliac artery lesions.Methods Between June 2006 and June 2010,26 patients with a total of 31 limbs of the TASC-D iliac artery lesions underwent endovascular procedure. Results Technical success rate was 90.3% (28/31).Clinical symptom improvement rate was 100%. Forty-four stents were placed in 28 limbs of the 24 patients.Eleven patients underwent an associated procedure; femoropopliteal endovascular procedures in 8 cases,femoro-popliteal bypass in 3 cases. Eight patients underwent an intravascular ultrasound thrombolysis.Postoperative ankle-brachial index ( ABI) was increased by more than 0. 15 in all patients. Twenty-two patients(26 limbs) were followed-up for 3 -44 mos, the primary patency rate at 12 months was 90% ,and cumulative patency rate was 95%. At 36 months primary patency rate was 70% , and secondary patency rate was 80%. Conclusions Because the technical success rate is high, endovascular techeniques are the choice of therapy in high risk patients with TASC-D type iliac artery lesions.     

The effects of β-NGF controlled delivery system on growth of primary cultured chick embryo dorsal root ganglion axons

Objective To construct β-NGF controlled delivery system and evaluate the biological effects of β-NGF on the growth of chick embryo dorsal root ganglion (DRG) axons in vitro. Methods Delivery systems releasing β-NGF at 50/μL, 100/μg/L and 250 μg/L concentration were constructed. To determine the optimal dose response effects of NGF in the controlled delivery system, DRG were co-cultured with of β-NGF at above concentrations while using DRG basic culture as control. Axonal growth was observed. DRG were also cocultured with the components in the controlled delivery system to detect the effects on growth of DRG axons. The experiment was divided into 5 experimental groups and 1 control group: control group, DRG+ fibrin; Group A,DRG+ fibrin+ peptide + heparin + 100 μg/L β-NGF; Group B, DRG + fibrin + heparin + 100/μg/L β-NGF;Group C, DRG + fibrin + peptide + 100 μg/L β-NGF; Group D, DRG + fibrin + 100 μg/L β-NGF; Group E,DRG + fibrin + peptide + heparin. Results The growth of DRG axons in 50 μg/L, 100μg/L and 250/μg/Lconcentration of β-NGF controlled delivery system was 1.31 ( P > 0. 05), 3.78 ( P < 0. 01 ) and 3.05 ( P <0.01) folds of the control respectively. The growth of DRG axons in 100 μg/L group was significantly better comparing to that in 250 μg/L group. The growth of DRG axons in Groups A, B, C, D and E was 3.75, 1.15,1.12, 1.10 and 1.09 folds of the control group, respectively. The difference was only statistically significant between Group A and the control group ( P < 0. 01 ). Conclusion β-NGF released from the β-NGF controlled delivery system was bioactive. It could promote the growth of DRG axons.     

立体定向显微手术治疗大脑半球运动区病灶

1对象与方法我科于2003年5月～2005年12月．对20例大脑半球运动区病灶应用立体定向脑沟入路开颅病灶切除术，其中男11例，女9例；年龄15～69岁，平均41岁。临床表现为癫痫大发作8例．部分性发作12例。病灶直径10～20mm 4例．21～30mm／113例，〉30mm／13例。病灶均位于皮质下．硬膜切开后肉眼均不能识别。     

气源质量--气动阀门寿命的保障

气动阀门的气缸和定位器，其可靠性和寿命是衡量产品质量的主要指标，然而现场往往是由于气源质量不好而造成故障，这已成为某些工厂的一大问题。本文对此作了分析举例，文后对气源处理水平的提高作了一些建议。     

泌尿系结石腔镜手术中肾盂内压力的研究进展

泌尿系结石是泌尿系统常见的疾病之一,大部分为上尿路结石。随着泌尿系结石微创技术的不断推广,输尿管镜碎石术及经皮肾镜碎石术等泌尿腔镜手术已广泛应用于上尿路结石的治疗中。上尿路结石腔镜手术中容易引起肾盂内压力(renal pelvic pressure,RPP)的升高,增加术后并发症的发生,造成肾脏一定的损害,甚至导致致死性尿源性脓毒症及感染性休克,严重威胁患者的生命。本文旨在对泌尿系结石腔镜手术中RPP的相关研究做一综述。