大鼠胰腺癌微环境诱导未成熟树突状细胞功能的变化

Objective To study the effect of pancreatic cancer microenvironment on maturation of dendritic cells ( DCs), and to investigate the mechanism by which tumors escape from immune recognition. Methods DCs were cultured with rmGM (40μg/L) ,rmIL-4 (40μg/L) ,and the supernatant of cell line AR42J cells was added on the day 6, then a large number of DCs was gained. DCs cultured and in-duced by routine methods were used as controls. The expression of surface molecules CD86, and CD80 in DCs was detected to observe whether this intervention could delay or block maturation of DCs and this effect could be reversed by stimulation with lipopolysaccharide (LPS). T cell stimulatory effects were also determined. Results Compared to normal DCs, CD86 and CD80 expression positive rate was significantly decreased in DCs induced by AR42J supernatant from (70.88±3.60)% to (7.15±0.71 )%. After stimulation with LPS, the expression of CD86, and CD80 in DCs was still low, suggesting the AR42J super-natant could block the maturation of DCs. The allostimulatory capacity of the immature DCs stimulated with the AR42J superoatant was significantly lower than that of the normal immature DCs. Conclusion The supernatant from pancreatic cancer ceils in rats could inhibit the maturation of DCs, which was not easily reversed.     

大鼠胰腺癌微环境诱导未成熟树突状细胞功能的变化

Objective To study the effect of pancreatic cancer microenvironment on maturation of dendritic cells ( DCs), and to investigate the mechanism by which tumors escape from immune recognition. Methods DCs were cultured with rmGM (40μg/L) ,rmIL-4 (40μg/L) ,and the supernatant of cell line AR42J cells was added on the day 6, then a large number of DCs was gained. DCs cultured and in-duced by routine methods were used as controls. The expression of surface molecules CD86, and CD80 in DCs was detected to observe whether this intervention could delay or block maturation of DCs and this effect could be reversed by stimulation with lipopolysaccharide (LPS). T cell stimulatory effects were also determined. Results Compared to normal DCs, CD86 and CD80 expression positive rate was significantly decreased in DCs induced by AR42J supernatant from (70.88±3.60)% to (7.15±0.71 )%. After stimulation with LPS, the expression of CD86, and CD80 in DCs was still low, suggesting the AR42J super-natant could block the maturation of DCs. The allostimulatory capacity of the immature DCs stimulated with the AR42J superoatant was significantly lower than that of the normal immature DCs. Conclusion The supernatant from pancreatic cancer ceils in rats could inhibit the maturation of DCs, which was not easily reversed.     

大鼠胰腺癌微环境诱导未成熟树突状细胞功能的变化

Objective To study the effect of pancreatic cancer microenvironment on maturation of dendritic cells ( DCs), and to investigate the mechanism by which tumors escape from immune recognition. Methods DCs were cultured with rmGM (40μg/L) ,rmIL-4 (40μg/L) ,and the supernatant of cell line AR42J cells was added on the day 6, then a large number of DCs was gained. DCs cultured and in-duced by routine methods were used as controls. The expression of surface molecules CD86, and CD80 in DCs was detected to observe whether this intervention could delay or block maturation of DCs and this effect could be reversed by stimulation with lipopolysaccharide (LPS). T cell stimulatory effects were also determined. Results Compared to normal DCs, CD86 and CD80 expression positive rate was significantly decreased in DCs induced by AR42J supernatant from (70.88±3.60)% to (7.15±0.71 )%. After stimulation with LPS, the expression of CD86, and CD80 in DCs was still low, suggesting the AR42J super-natant could block the maturation of DCs. The allostimulatory capacity of the immature DCs stimulated with the AR42J superoatant was significantly lower than that of the normal immature DCs. Conclusion The supernatant from pancreatic cancer ceils in rats could inhibit the maturation of DCs, which was not easily reversed.     

移植肾初期肾动脉狭窄的超声诊断

目的 探讨肾移植术后初期肾动脉狭窄的超声诊断。方法 回顾分析厦门大学附属翔安医院2019年4月-2021年12月超声科检查的103例肾移植患者的超声及临床资料。结果 共28例超声提示肾主动脉或动脉吻合口血流信号紊乱及PSV增快,PSV>250cm/s。其中11例在术后3个月的超声定期监测中PSV处于稳定增快状态,最终经DSA或CTA检查,动脉吻合口狭窄8例,肾主动脉距吻合口5mm处狭窄1例。另2例PSV增快为肾主动脉成角所致。其余17例在术后3个月的超声定期监测中,PSV逐渐下降,最终PSV≤250cm/s。肾主动脉PSV持续增快病例组9例术后肌酐值下降缓慢未达正常范围;肾动脉PSV逐渐下降组,肌酐值随之降至正常范围。超声显示PSV增快及血流信号紊乱的病例中真性狭窄的比例为32.1%(9/28)。结论 肾移植术后短期内吻合口及其旁软组织增厚水肿可致肾动脉PSV增快及紊乱血流信号,并非一定是真性狭窄。肾移植后初期肾主动脉容易成角导致PSV增快。术后初期应用超声监测肾动脉PSV变化趋势尤为必要,减少侵入性检查。超声监测中结合肌酐值的变化趋势对移植肾动脉狭窄的判定有重要意义。