颈总动脉断裂一例救治后反思

1病例资料男,35岁。被刀刺伤左颈部和左肩部,颈部喷射状出血,肩部漏气,伤后即刻入院。查体:意识不清,血压测不到,颈、肩部出血不止。立即建立静脉液路,伤口填塞压迫止血。抢救过程中间断出现呼吸暂停,快速补液1200ml后恢复血压(60/40mmHg)、呼吸(40/min),床旁摄X线胸片提示开放     

海南省某医院103株鲍曼不动杆菌复合群-内酰胺类耐药性与碳青霉烯酶携带情况分析

目的 鲍曼不动杆菌复合群细菌是临床可造成感染的重要条件性致病菌,本研究旨在调查和分析我国海南省临床来源鲍曼不动杆菌复合群细菌对常用-内酰胺类药物的耐药性及菌株碳青霉烯酶携带情况,探讨其耐药流行性特点。方法 采用微量肉汤稀释法对103株采集自2012-2013年海南省三亚市人民医院的非重复鲍曼不动杆菌复合群细菌进行7种抗菌药物的药敏检测;应用聚合酶链反应(PCR)对收集的鲍曼不动杆菌复合群细菌进行9种碳青霉烯酶编码基因的筛查。结果 103株鲍曼不动杆菌复合群细菌对哌拉西林、头孢他啶、头孢噻肟的耐药率较高(接近50%),对头孢吡肟、哌拉西林/他唑巴坦的耐药率为40%,对亚胺培南和美罗培南的耐药率均在20%左右;PCR检测显示有38株携带blaOXA-58基因,62株blaOXA-66基因,其中25株同时携带有这两种基因;其余7种碳青霉烯酶编码基因筛查结果为阴性。结论 本研究中鲍曼不动杆菌复合群细菌对-内酰胺类药物耐药性较高,菌株携带的blaOXA-58和blaOXA-66基因编码的碳青霉烯酶对菌株的耐药表型有一定贡献意义,但仍有其他耐药机制参与到碳青霉烯酶耐药表型的贡献中。     

失眠及发作性嗜睡型睡眠紊乱治疗药物的研究概况

按化学结构分类概述失眠和发作性嗜睡型睡眠紊乱治疗药物的研究与开发。失眠和发作性嗜睡是常见的两种睡眠紊乱疾病,严重影响人类的身体健康和生活质量,并可导致其他疾病的发生和发展,应受到重视并得到及时而恰当的治疗。     

心律安口服液对心律失常的临床与实验研究

用成组对照的方法观察心律安口服液对各类心律失常病人的抗心律失常作用 ;对大鼠冠状动脉结扎致心律失常 ,用电刺激家兔心律失常 ,以观察心律安口服液对大鼠、家兔心律失常的保护作用。临床研究表明 :心律安对室性早搏、房性早搏、窦性心动过速有较好的疗效。实验研究提示 :心律安口服液能拮抗心肌缺血和心律失常 ,尤其对室性早搏有较好的疗效     

大学生视力不良476只眼散瞳验光结果分析

<正> 为了解大学生视力不良的屈光不正情况,做好近视眼防治工作,1989年9月,我们将入学新生体检中发现视力低于1.0者,进行了散瞳验光,所获资料结果分析报道如下。材料和方法采用国际标准视力表(照明度达600lux以上),测定入学新生视力,将视力低于1.0(不包括1.0)258人,476只眼列入验光范围,其中男性164人,297只眼;女性94人,179只眼。用2％阿托品眼药水散幢,带状光检影验     

Genistein对体外培养兔软骨细胞一氧化氮表达的影响

目的：观察受体酪氨酸激酶抑制剂genistein对体外培养兔软骨细胞一氧化氮（NO）表达的影响。方法：兔膝关节软骨细胞传代培养,应用不同浓度genistein干预1、2、3、4天后,通过亚硝酸盐测定检测培养上清液NO表达水平。结果：形态学、甲苯胺蓝染色显示3代以内体外培养软骨细胞保持细胞表型的稳定。在genistein干预后上清液中NO水平下降,下降水平与genistein干预浓度和时间相关。结论：体外培养兔软骨细胞三代以内的细胞具有较好的活性,genistein能有效降低兔软骨细胞NO的表达水平。     

大鼠异体肢体移植术后急性排斥反应阶段血管内皮细胞ICAM-1表达的动态变化

Objective To investigate the dynamic changes of intercellular adhesion molecule-1 (ICAM-1) expression in endothelial cells (EC) during hindlimb allograft acute rejection in rats and the inhibitory effect of cyclospofine A (CsA) on the acute rejection. Methods The rat model of hindlimb allograft was developed. The rats were randomly divided into following groups: control group (Wistar→Wistar), rejection group ( Sprague-Dawley→Wistar) and CsA-treated group (Sprague-Dawley→Wistar). At postoperative day 1, day 4 and day 7samples of the femoral artery from the allograft limb were harvested to observe the pathologic changes. ICAM-1 expression in EC was quantified using immunohistochemical assay. Results Slight swelling and weak ICAM-1expression of EC were observed in the control group. In the rejection groups, obvious EC swelling and massive lymphocyte infiltration were seen. ICAM-1 expression in EC was significantly stronger and elevated. In CsA-group only mild infiltration of lymphocytes was seen and ICAM-I expression in EC was also much weaker.Conclusion During rat hindlimb allograft acute rejection the expression of ICAM-1 in EC was closely related to the occurrence and development of acute rejection. CsA could reduce the expression of ICAM-1 in EC and hence inhibit acute rejection of composite allograft.     

大鼠异体肢体移植术后急性排斥反应阶段血管内皮细胞ICAM-1表达的动态变化

Objective To investigate the dynamic changes of intercellular adhesion molecule-1 (ICAM-1) expression in endothelial cells (EC) during hindlimb allograft acute rejection in rats and the inhibitory effect of cyclospofine A (CsA) on the acute rejection. Methods The rat model of hindlimb allograft was developed. The rats were randomly divided into following groups: control group (Wistar→Wistar), rejection group ( Sprague-Dawley→Wistar) and CsA-treated group (Sprague-Dawley→Wistar). At postoperative day 1, day 4 and day 7samples of the femoral artery from the allograft limb were harvested to observe the pathologic changes. ICAM-1 expression in EC was quantified using immunohistochemical assay. Results Slight swelling and weak ICAM-1expression of EC were observed in the control group. In the rejection groups, obvious EC swelling and massive lymphocyte infiltration were seen. ICAM-1 expression in EC was significantly stronger and elevated. In CsA-group only mild infiltration of lymphocytes was seen and ICAM-I expression in EC was also much weaker.Conclusion During rat hindlimb allograft acute rejection the expression of ICAM-1 in EC was closely related to the occurrence and development of acute rejection. CsA could reduce the expression of ICAM-1 in EC and hence inhibit acute rejection of composite allograft.     

上海海运学院师生性知识来源与性健康教育的需求

目前性健康教育已成为健康教育的热点与难点之一，特别对其时与度的把握困扰着人们。为此我们对602名大学师生进行相关内容的调查。1材料和方法采用分层抽样方法，抽取上海海运学院393名1－4年级大学生。其中男性180名，女性213名，平均年龄为20．50岁；抽取上海海运学院文、理科及基础部教师209名，其中男性102名，女性107名，已婚132名，未婚77名。已婚者平均年龄42．96岁，未婚者平均年龄26．50岁。发放自编的调查问卷，主要内容有（1）性知识的主要来源（允许有多项选择）；（2）这些性知识是否有用及是否健康（规定各选一个答案）；（3…     

更年期综合征辨证分型及相关指标的研究进展

收集1981年以来有关更年期综合征辨证分型及相关指标研究的文献资料，从辨证分型的研究、客观检测指标的研究、病理生理学的研究等三方面进行了综述，同时提出了目前在本病辨证分型方面存在的一些问题和建议。