“利咽止敏方”治疗过敏性咽炎30例临床研究

目的:探讨利咽止敏方治疗过敏性咽炎的临床疗效。方法:将60例过敏性咽炎患者随机分为治疗组与对照组各30例,对照组采用西药西替利嗪口服,治疗组予中药利咽止敏方口服。2组均治疗4周后比较临床疗效、症状积分改善情况,随访3个月比较2组复发率。结果:对照组总有效率为73.33%,治疗组总有效率为90.00%,组间比较差异有统计学意义(P0.05)。2组患者治疗后咽痒、刺激性干咳症状积分均明显低于治疗前(P0.05),治疗组对症状积分的改善优于对照组(P0.05)。3个月后,对照组复发率为54.5%明显高于治疗组的14.8%(P0.05)。结论 :利咽止敏方治疗过敏性咽炎疗效明显优于西替利嗪,同时能有效改善患者症状,减轻患者痛苦,值得临床推广。     

环状RNA 0001846对骨关节炎生物学功能的影响及其机制

目的探讨环状RNA 0001846(circ₀001846)调控滑膜巨噬细胞极化在骨关节炎中的生物学功能及其机制。方法收集常州市第二人民医院2021年12月至2022年9月行全膝关节置换术骨关节炎患者及遭受膝关节创伤且无骨关节炎患者的滑膜组织各10例, 将10例骨关节炎滑膜组织作为研究组, 10例非关节炎滑膜组织作为对照组。使用实时定量聚合酶链反应(RT-qPCR)测定环状RNA(circRNA)及巨噬细胞标志物在滑膜组织的mRNA表达水平;使用THP-1细胞上清液孵育软骨细胞, 通过细胞计数试剂盒(CCK-8)与流式细胞术测定软骨细胞活力、软骨细胞凋亡率;蛋白质印迹法(Western blot)检测软骨细胞凋亡相关蛋白表达水平。组间比较采用t检验。结果实验组患者滑膜组织中M1巨噬细胞标志物表达高于对照组[诱导型一氧化氮合酶(iNOS):2.67±0.34比0.98±0.10, t=15.140, P<0.01;肿瘤坏死因子-α(TNF-α):2.51±0.31比1.02±0.22, t=12.422, P<0.01和白细胞介素(IL)-1β:4.27...     

紧密连接蛋白在声带白斑伴咽喉反流患者声带黏膜中的表达

Abstract:ObjectiveTo study the roles of tight junction proteins (TJPs) in vocal mucosal barrier damage via probing their differential expressions in vocal mucosa of patients suffering from vocal leukoplakia accompanied with laryngopharyngeal reflux (LPR).Methods26 patients suffering from vocal leukoplakia were divided into two groups (i.e., groups with and without LPR) (13 cases in each group) according to the results of 24 h esophageal pH monitoring. Mucosal specimens of vocal cord were collected from all the patients during operation. HE staining was used to score the pathological changes of the mucosa. Transmission electron microscope (TEM) was utilized to observe the morphology of mucosal tight junction, and Western blot to detect the expressions of Claudin 1 and ZO 1.ResultsCompared with the group without LPR, marked inflammatory cell infiltration and poor structural integrity of the epithelium were observed via HE staining, TEM demonstrated significant decrease of electron dense materials around the tight junction and obvious morphological changes of the tight junction, and Western blot showed decreased expressions of Claudin 1 and ZO 1 in the specimens from the group with LPR. Furthermore, immunofluorescence assay confirmed that expressions of Claudin 1 and ZO 1 were decreased within cytoplasm in those from the group with LPR.ConclusionRefluxed materials may cause structural damage of tight junction and abnormal expressions of TJPs, thus leading to structural damage of intercellular junction, intercellular spaces dilatation and enhancement of epithelial permeability, which may be an important mechanism of vocal mucosal barrier damage in vocal leukoplakia accompanied with LPR.     

变应性鼻炎继发哮喘患者血浆microRNA表达谱的改变

目的应用microRNA表达谱芯片技术，选取临床明确诊断的变应性鼻炎（allergic rhinitis, AR）及变应性鼻炎罹患哮喘(asthma, AS)患者相应血浆，分析变应性鼻炎患者罹患哮喘后血浆microRNA的差异性表达。方法选取AR及AR+AS各3例，使用miRCURY LNATM芯片，筛选出两组患者血浆中差异表达的microRNA并进行聚类分析；最后通过生物信息学分析预测其靶基因。结果与AR组相比较，AR+AS组差异表达的microRNA共有9个，其中表达下调的有4个，表达上调的有5个。靶基因预测显示，以黏附连接、Wnt和MAPK信号传导通路靶基因的差异表达最为显著。结论通过microRNA芯片发现的相关差异性表达的microRNA，有可能成为AR患者罹患AS的易感性生物标志物及临床可能的治疗靶点，但是尚需要进一步靶基因验证及功能学分析。