散光表的设计及使用方法

在眼的屈光检查中,散光的检查占有很重要的位置。散光表为主观检查有无散光存在和有散光时判定其轴向位置的一种简便实用器具。目前国内尚无理想的散光表,也未见成品出     

11例骨原发性非何杰金氏淋巴瘤临床病理分析

骨原发性恶性淋巴瘤较少见,约占骨恶性肿瘤的2～5.4%,国外报道较多,国内自1956年以来仅报道50余例。我室20年中(1965～1985)遇到11例,结合文献报道如下。     

一种筛选鉴定重组载体的简便方法

一种筛选鉴定重组载体的简便方法田新霞张陵林陈杰吴浩强从原理上讲，克隆重组载体并不困难。但在实际工作中，传统方法如连接后转化细菌、选取若干克隆进行小样本制备来筛选含有目的基因的重组体的工作十分繁锁。如何区分含有目的基因的克隆，还是载体自身环化的集落，是...     

泌尿生殖道支原体感染率及药敏结果分析

目的　了解我地区Uu、Mh的感染情况 ,以及为其治疗提供敏感的抗生素。方法　用配套试剂盒对男性尿道分泌物、前列腺液、女性宫颈分泌物进行培养 ,并对 5 6例阳性标本做药物敏感试验。结果　总感染率为 4 0 .6 % ,男性感染率31.0 % ,女性感染率为 4 4 .8% ,二者差异有显著性 (P <0 .0 5 )。交沙霉素、强力霉素、美满霉素、阿奇霉素、左氧氟沙星、四环素、红霉素的耐药率分别是 :8.9%、2 1.1%、30 %、4 3.7%、5 3.5 %、6 3%、80 %。结论　目前泌尿生殖道感染率很高。治疗中不宜选用红霉素、交沙霉素、强力霉素、美满霉素可作为治疗支原体感染的首选药。     

CTT triplex系统各基因座位等位基因在葡萄胎基因组中的分布及其意义

我们对采用PCR和聚丙烯酰胺凝胶电泳鉴别出的37例DNA完全来自父方的遗传学完全性葡萄胎(g-CHM)进行基因组中CTTtriplex系统各基因座位(CSF1PO、TPOX和TH01)等位基因分布的分析,并初步研究了这些等位基因分布与临床预后的关系。结果显示,在37例g-CHM中,CSF1PO座位中3个等位基因(11,12和14)和TPOX座位中的1个等位基因(11)的出现率与它们在北京地区人群中的基因频率差异显著;g-CHM中CSF1PO、TPOX和TH01基因座位杂合度显著低于北京地区人群的杂合度(P值均远小于0.01);在23例良性g-CHM和10例侵袭性g-CHM中:CSF1PO座位的等位基因10、11在良性中的出现率高于在侵袭性g-CHM中(P=0.026148),等位基因12在良性中的出现率低于在侵袭性g-CHM中(P=0.023879);TPOX座位的等位基因8在良性中的出现率高于在侵袭性g-CHM中(P=0.004322),而等位基因11在良性中的出现率低于其在侵袭性g-CHM中(P=0.008671)。上述结果提示,葡萄胎基因组中存在某些等位基因分布与在人群中的分布不同,而且葡萄胎是否具有侵袭性也和某些等位基因的出现率过高或过低有相关性,这些与侵袭性有关的等位基因可能成为预测葡萄胎是否具有侵袭性的标志物。     

Effect of ipratropium bromide on airway and pulmonary muscarinic receptors in a rat model of chronic obstructive pulmonary disease

Objective　To observe the level of muscarinic receptors in airway and lung tissues, and the effect of inhaled ipratropium bromide on these receptors in a rat model of chronic obstructive pulmonary disease (COPD). Methods　This model was developed by exposure of rats to 250 ppm SO2 gas, 5?h/d, 5d/wk, for a period of 7 wk. The COPD rats inhaled 0.025% aerosolized iratropium bromide for 20 min, 2 times daily, in an airtight chamber. Muscarinic receptors in airway and lung tissues of normal rats, ipratropium bromide-treated COPD rats and the recovering COPD rats were measured by the radio-ligand binding assay. Results　Airway/lung pathology and pulmonary function tests showed that chronic SO2 exposure caused pathophysiologic changes similar to those observed in human COPD. The density (0.038±0.011, pmol/mg protein) and affinity (Kd, 23±11 pmol/L) of muscarinic receptors in airway and lung tissues of COPD rats were not changed compared with those of normal control rats (0.030±0.008 and 29±19, respectively, P&gt;0.05). Densities of the muscarinic receptors were not changed after inhalation of ipratropium bromide for 5 days, but increased significantly after inhalation for 30 days, as compared with those of the untreated COPD rats. The muscarinic receptors returned the normal levels at day 6 after cessation of ipratropium bromide treatment. There were no differences among different groups of rats in equilibrium dissociation constants (Kd). Conclusion　A rat model of COPD with pathophysiologic changes similar to the human counterpart was developed using chronic SO2 exposure. There was no significant change in the number and function of muscarinic receptors in airway and lung tissues of the COPD rats, but upregulation of the muscarinic receptors was observed after long-term inhalation of ipratropium bromide.     

同种原位心脏移植的近远期免疫抑制治疗探讨

目的：探讨同种原位心脏移植术后免疫抑制剂的应用问题,方法：对1例同种原位心脏移植的病人采用环孢素A,硫唑嘌呤,皮质类固醇及OKT3四联疗法进行免疫抑制治疗。结果：心脏移植术后1a6个月未发生明显的急性排异反应。结论;根据临床表现及综合监测调整免疫抑制剂的用量可以有效地抗排异反应。