4种病原菌特异基因片段阳性蜱的16S rRNA基因克隆文库分析

Objective To develop the method of 16S rRNA gene clone library for tick bacterial flora analysis, and to analyze the detection effective of pathogens in tick and capacity of bacterial flora diversity. Methods Primers were designed according to the specific gene of Borrelia burgdorferi, Bartonella henselae, Anaplasma phagocytophilum, Ehrlichia chaffeensis and templates were choosen by positive PCR result to amplify the DNA extracted from the ticks. One set of primers targeting 16S rRNA gene conserved region were chosen to amplify certain fragments, DNA extraction, PCR reaction, cloning and sequencing. Nucleotide sequences were compared with GenBank database. Calculated Coverage values of clone library and Shannon-Wiener diversity index. Results Sixteen defined genus-or species-bacteria were detected in 103 valid sequences. Eight species were edge type (Clone No. > 5). Three kinds of pathogens were identified (Borrelia burgdorferi, Bartonella henselae and Rickettsia sp). Three kinds of pathogens were not edge type(Clone No. < 5). Coverage value was 96.11%, and Shannon-Wiener index was 2.40. Analysis results of cloning sequence showed that tick-parasitic bacteria mainly were α and γ deformation mycetes which accounted for 56.25% (9/16). Conclusions The 16S rRNA gene sequences technology could make relative quantitative of bacterial flora, and detect many kinds of pathogens in tick. It's a good method for detection of pathogens and bacterial flora analysis.     

重型颅脑损伤患者的预后因素研究

目的探讨重型颅脑损伤的患者预后的临床影响因素。方法回顾性分析我院神经外科2008年2月—2012年2月收治的368例重型颅脑损伤患者的临床资料。结果患者年龄、入院时间、瞳孔、血氧分压、收缩压、呼吸变化、格拉斯哥昏迷评分(GCS)、中线移位程度、基底池的变化程度、血肿部位、是否手术治疗、血肿量、术后营养的及时供给、多发伤或伴有严重并发症等因素均是影响重型颅脑损伤预后的相关因素。结论伤后尽量缩短入院时间,适当放宽手术指征,早期预防、处理并发症以及早期营养的供给、早期康复训练,能够促使重型颅脑损伤患者得到及时有效的救治及患者的预后良好率,提高生存质量。     

山莨菪碱致过敏性休克1例

<正> 患者女,31岁,主因劳累过度、头晕、头痛、恶心一天。查体:血压 14.6/9.6kPa(即110/70毫米汞柱),心率45次/分,神经系统(—)。于1994年2月14日11时印象为“窦性心动过缓”收入院治疗,给     

原发性肝癌特殊首发症状误诊分析

原发性肝癌特殊首发症状误诊分析徐吉兰，刘宝民，张景山，张秀云２５１１００山东省齐河县人民医院典型的原发性肝癌（下称肝癌）不难诊断。该病早期症状隐匿，且有少数病例临床表现特殊，易致误诊。为提高对本病的认识，现将我院以特殊首发症状误诊的病例结合文献简述如...     

南京市瓶装饮用纯净水卫生状况分析

为了解南京市纯净水卫生状况，对南京市站检测的７０份样品的检测结果进行了分析，结果表明样品合格率为４２．９％，监督和委托样品合格率有显性差异，主要不合格指标为微生物指标。     

南京市公共场所卫生状况调查

对全市部分公共场所卫生状况抽样调查结果显示，理化指标和空气细菌数合格率为８６．６％；公共用品用具细菌总数合格率为９２．５％，大肠菌群阳性率为１２．２％。存在问题较大的是商场的噪声，展览馆的噪声和照度，以及旅店为珠枕套、脸池、浴缸，化娱乐业的茶怀，桑拿拖鞋，理发剪刀的消毒效果。     

门冬氨酸鸟氨酸对肝切除术后肝功能保护作用的研究

Objective To investigate the protective effects of L-Ornithine-L-Aspartate on liver function in patients after liver resection. Methods Eighty-two patients undergoing liver resection were randomly divided into study group and control group. Control group was treated with conventional therapy and study group had conventional therapy plus L-Ornithine-L-Aspartate for injection intravenously from the first day before operation to the seven day after operation. The fasting alanine aminotransferase (ALT), aspartate aminotransferase ( AST ), γ-glutamyl transpeptadase(GGT), total bilirubin(TB), direct bilirubin(DB) and blood ammonia(BA) levels were determined before operation and on days 1, 3 and 7 after operation. Results On days 1, 3 and 7 after operation, ALT and AST levels in the study group [ALT: ( 115.58 ± 69. 20), (81. 36 ± 14. 55 ), ( 61.24 ± 11. 32) U/L; AST: ( 127.29 ±33.61 ), (95.26 ± 16.83 ), (55.16 ± 14.83 )U/L] were significantly lower than those in the control group[ALT:(175.27 ±68.35), (97.37 ±34.24), (78.28 ± 19. 73) U/L;AST: (152. 29 ±31.65), (115.28 ±35.24),(82.49 ± 28.18) U/L] (P ＜ 0.05 ). In the study group of the porta blocked over 15 min, levels of AST, ALT were obviously lower than that of the control group( P ＜ 0. 05 ). Conclusions L-Ornithine-L-Aspartate for injection can alleviate the hepatic dysfunction after hepatectomy, especially in the long time of porta blocked and has protective advantage for liver function.     

双黄连粉针治疗婴幼儿腹泻114例疗效观察

婴幼儿腹泻是儿科常见病 ,近 2年来我们应用双黄连粉针治疗婴幼儿腹泻 114例 ,现报告如下。1　临床资料选择自 1997- 11～ 1999- 10我院儿科腹泻患儿住院病例 ,并且具备下列条件 :(1)年龄≤ 2岁 ;(2 )急性起病 ,腹泻病程 5ｄ以内 ;(3)大便次数≥ 5次 /ｄ ,粪便呈稀水样和蛋花汤样。共选择 193例做为观察对象。随机分为治疗组 114例 ,对照组 6 9例 ,进行系统治疗。治疗组 :男 82例 ,女 32例 ;年龄≤ 6个月 36例 (31 5 8% ) ,≤ 1岁 5 0例 (4 3 86 % ) ,≤ 2岁 8例(2 4 5 6 % )。对照组 :男 44例 ,女 2 5例 ;年龄≤ 6个月 15例(2 1 74% ) ,≤ …     

大厂镇地区饮用水源枯水期水质评价

［目的］了解和掌握大厂镇地区拟建取水口水源的水质情况。［方法］在长江枯水期对该水源地按不同继面、不同层次、不同潮位及取水口断面不同位置的水样进行了监测,依据ＧＢ３８３８－１９８８《地面水环境质量标准》Ⅱ类水质评价。［结果］达标率为９０．１１％。超标项目为溶解性铁、五日生化需氧量（ＢＯＤ５）、石油类、非离子氨和总大肠菌群。表层水样石油类含量显著高于中层和深层水样。不同潮位水样水质间无显著性差异。［结     

4种病原菌特异基因片段阳性蜱的16S rRNA基因克隆文库分析

Objective To develop the method of 16S rRNA gene clone library for tick bacterial flora analysis, and to analyze the detection effective of pathogens in tick and capacity of bacterial flora diversity. Methods Primers were designed according to the specific gene of Borrelia burgdorferi, Bartonella henselae, Anaplasma phagocytophilum, Ehrlichia chaffeensis and templates were choosen by positive PCR result to amplify the DNA extracted from the ticks. One set of primers targeting 16S rRNA gene conserved region were chosen to amplify certain fragments, DNA extraction, PCR reaction, cloning and sequencing. Nucleotide sequences were compared with GenBank database. Calculated Coverage values of clone library and Shannon-Wiener diversity index. Results Sixteen defined genus-or species-bacteria were detected in 103 valid sequences. Eight species were edge type (Clone No. > 5). Three kinds of pathogens were identified (Borrelia burgdorferi, Bartonella henselae and Rickettsia sp). Three kinds of pathogens were not edge type(Clone No. < 5). Coverage value was 96.11%, and Shannon-Wiener index was 2.40. Analysis results of cloning sequence showed that tick-parasitic bacteria mainly were α and γ deformation mycetes which accounted for 56.25% (9/16). Conclusions The 16S rRNA gene sequences technology could make relative quantitative of bacterial flora, and detect many kinds of pathogens in tick. It's a good method for detection of pathogens and bacterial flora analysis.