自发性高血压大鼠缺血再灌注后视网膜毛细血管细胞凋亡及p53基因表达

Objective To investigate the effect of apoptosis-related gene p53 on the apoptosis of retinal capillary cells in rats with spontaneously hypertensive (SHR) after ischemic reperfusion injury. Methods A total of 60 SHR rats were randomly divided into sham group (SHR-SH) and retinal ischemie reperfusion group (SHR-RIR), which were subdivided into 5 subgroups according to the time after RIR: 2, 6, 24, and 72 hours and 7 days, with 6 rats in each subgroup. Another 60 Wistar-Kyoto (WKY) rats were divided into the same groups as the SHR rats as the control. The RIR model was set up. The apoptosis of retinal capillary cells was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) methods and the expression of p53 was determined by streptavidin-perosidase (SP) immunohistochemistry. Results The apoptosis rate of retinal capillary cells in the 5 SHR-RIR groups was (8.64±0.56)%, (14.92±0.99)%, (24.72±2.98)%, (16.53±1.80)%, and (7.12±1.10)%,respectively. The expression of p53 in SHR-RIR groups increased at the 2nd hour after RIR, reached the peak at the 24th hour, kept the high level at the 72nd hour, and remained a little at the 7th day, which was significantly different from which in the SHR-SH groups (P<0. 01). The expression of p53 were higher in SHR-IR groups than that in the WKY-RIR groups (P<0. 01). Conclusions p53 may play a part in RIR injury by inducing or promoting apoptosis. The apoptosis of retinal capillary cells after RIR is more severe under the hypertension, and reaches the peak at the 24 hour after RIR.     

浅谈《金匮要略》临床见习课小班化教学的设计与实践

正《金匮要略》(以下简称《金匮》)是中医学专业的必修课,临床基础课,课程文字枯燥,理论深奥,教师难讲,学生难学。在课堂教学中适当穿插临床见习课,有利于提高学生学习的兴趣,加深对基础理论的理解,培养中医临床思维,巩固专业思想。广州中医药大学第一临床医学院金匮要略教研室是全国中医高等院校中最早拥有独立临床教学基地的单位,目前在第一附属医院设置病床61张,为上好临床见习课提供了硬件保障。然而,近年来,由于学     

骨科治疗专用车的研制和应用

目的介绍一种骨科治疗专用车的研制方法并探讨其应用效果。方法研制骨科治疗专用车。采用自身对照法,对30例双下肢开放性骨折需在病房换药的患者,每个患者固定一位医生换药2次,首次换药使用骨科治疗专用车为试验组,再次换药使用一般治疗车为对照组。结果试验组换药时间和所用换药物品浪费数量显著少于对照组,患者及医生的满意度均高于对照组(均P<0.01)。结论骨科治疗专用车有助于减少换药物品浪费,节约患者医疗费用,缩短了医生操作时间,减轻医生的工作量,提高了患者及医生的满意度。     

自发性高血压大鼠缺血再灌注后视网膜毛细血管细胞凋亡及p53基因表达

Objective To investigate the effect of apoptosis-related gene p53 on the apoptosis of retinal capillary cells in rats with spontaneously hypertensive (SHR) after ischemic reperfusion injury. Methods A total of 60 SHR rats were randomly divided into sham group (SHR-SH) and retinal ischemie reperfusion group (SHR-RIR), which were subdivided into 5 subgroups according to the time after RIR: 2, 6, 24, and 72 hours and 7 days, with 6 rats in each subgroup. Another 60 Wistar-Kyoto (WKY) rats were divided into the same groups as the SHR rats as the control. The RIR model was set up. The apoptosis of retinal capillary cells was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) methods and the expression of p53 was determined by streptavidin-perosidase (SP) immunohistochemistry. Results The apoptosis rate of retinal capillary cells in the 5 SHR-RIR groups was (8.64±0.56)%, (14.92±0.99)%, (24.72±2.98)%, (16.53±1.80)%, and (7.12±1.10)%,respectively. The expression of p53 in SHR-RIR groups increased at the 2nd hour after RIR, reached the peak at the 24th hour, kept the high level at the 72nd hour, and remained a little at the 7th day, which was significantly different from which in the SHR-SH groups (P<0. 01). The expression of p53 were higher in SHR-IR groups than that in the WKY-RIR groups (P<0. 01). Conclusions p53 may play a part in RIR injury by inducing or promoting apoptosis. The apoptosis of retinal capillary cells after RIR is more severe under the hypertension, and reaches the peak at the 24 hour after RIR.     

健康教育联合放松训练对老年妇科肿瘤术后化疗患者心理状况及生存质量影响

目的探讨健康教育联合放松训练对老年妇科肿瘤术后化疗患者心理状况及生存质量的影响。方法选取本院2013年7月~2017年7月收治的80例老年妇科肿瘤术后化疗患者,随机分为研究组40例和对照组40例。研究组患者进行健康教育联合放松训练,对照组患者进行常规护理。对两组患者治疗后1周进行基线调查,护理3个月后进行癌症患者生活质量核心问卷(QLQ-C30)调查,多维健康心理控制源量表(MHLC)调查,自评抑郁量表(SDS)调查。结果研究组患者干预后的总体健康/生存质量量表(QL)、躯体功能(PF)、角色功能(RF)、情绪功能(EF)、认知功能(CF)得分均升高,SDS比干预前明显减少,差异有统计学意义(P0.05);对照组患者PF、RF、EF、CF、社会功能(SF)、疲乏(FA)、恶心呕吐(NV)、疼痛(PA)、呼吸困难(DY)、睡眠障碍(SL)、食欲丧失(AP)、便秘(CO)、对经济困难的感知(FI)以及SDS的变化差异无统计学意义(P0.05)。研究组患者干预后PF、RF、EF、CF、SF、腹泻(DI)、总体健康/QL得分均比对照组高,差异有统计学意义(P0.05)。研究组患者干预后FA、NV、PA、DY、SL、AP、CO、FI得分均比对照组降低,差异有统计学意义(P0.05)。自评抑郁量表(SDS)明显比对照组低,差异有统计学意义(P0.05)。研究组患者干预后有势力他人量表(PHLC)得分比干预前明显增高,差异有统计学意义(P0.05),机遇量表(CHLC)得分比干预前明显下降,差异有统计学意义(P0.05)。研究组患者内控量表(IHLC)、PHLC得分明显比对照组更高,差异有统计学意义(P0.05)。CHLC得分明显比对照组低,差异有统计学意义(P0.05)。结论健康教育联合放松训练对老年妇科肿瘤术后化疗患者心理状况及生存质量有较好的影响,可以减轻患者抑郁症状和不良情绪,是很好的化疗辅助护理方法,值得在医学领域推广和应用。     

掌叶大黄RpNAC1基因的克隆、亚细胞定位及表达分析

目的 克隆掌叶大黄Rheum palmatum转录因子基因RpNAC1,进行生物信息学、亚细胞定位及表达模式分析。方法 根据转录组中一个NAC unigene,利用RT-PCR克隆开放阅读框（open reading frame,ORF）。通过生物信息软件预测基因编码蛋白的理化性质、结构域等分子特征。采用DNAStar 6.0和MEGA 7.0分别进行氨基酸序列比对和进化分析。构建绿色荧光蛋白（GFP）融合表达载体,以农杆菌侵染烟草叶片的瞬时表达法分析亚细胞定位。运用qRT-PCR检测基因表达模式。结果 分离到RpNAC1基因,ORF（1269 bp）,编码一个由422个氨基酸组成的蛋白质,相对分子质量47 070,等电点5.80,包含一个保守NAC结构域（32～181）,与多种植物NAC蛋白一致性较高（62.42%～88.7%）,聚在植物NAC分子进化树的NAC2分支,与甜菜NAC（XP_010694507）亲缘关系较近。RpNAC1-GFP融合蛋白定位在烟草细胞核内。RpNAC1基因在一年生植株根中表达量最高,根茎中表达量最低,相对表达量分别为叶中的5.37、0.012倍;该基因响应200 μmol/L赤霉素（gibberellin,GA3）处理后在24 h内总体上调,200 μmol/L茉莉酸甲酯（methyl jasmonate,MeJA）处理1 h和12 h基因显著上调,200 μmol/L水杨酸（salicylic acid,SA）处理12 h显著诱导基因表达,200 μmol/L脱落酸（abscisic acid,ABA）处理抑制基因表达,200 μmol/L乙烯（ethylene,ET）处理未见明显变化。结论 获得掌叶大黄RpNAC1基因序列及表达特征,为进一步研究其在蒽醌类成分合成与积累中的转录调控作用提供支撑。     

自发性高血压大鼠缺血再灌注后视网膜毛细血管细胞凋亡及p53基因表达

目的 探讨凋亡相关基因p53在自发性高血压大鼠(SHR)视网膜缺血再灌注损伤（RIR）后视网膜毛细血管细胞凋亡中的作用。方法 将60只SHR随机分为假手术组(SHR-SH)和缺血组(SHR-RIR),每组各30只大鼠;每组又按照不同再灌注时间分别再分为再灌注后2、6、24、72 h和7 d共5个小组,每小组各6只大鼠。选取60只Wistar-Kyoto大鼠（WKY）同样分为假手术组(WKY-SH)和缺血组(WKY-IIR)作为对照。建立大鼠RIR损伤动物模型,采用末端脱氧核苷酸转移酶介导的dUTP 缺口翻译法（TUNEL）法观察大鼠视网膜毛细血管细胞凋亡,通过免疫组织化学链酶卵白素-生物素复合体法(SP)检测RIR后不同时段视网膜组织中p53基因的表达变化。结果 SHR视网膜毛细血管细胞凋亡率在RIR后2、6、24、72 h和7 d时分别为（8.64±0.56）%、（14.92±0.99）%、（24.72±2.98）%、（16.53±1.80）%和（7.12±1.10）%。SHR视网膜毛细血管在RIR后2 h p53表达即开始增加,6 h继续增多,24 h达到高峰,72 h仍维持在较高水平,7 d时仍有少量表达,与SHR SH比较,差异有统计学意义（P＜0.01）。WKY-SH和SHR-SH组各时间点细胞凋亡和p53表达无明显差异。WKY RIR与SHR RIR相应时间点比较,差异有统计学意义（P＜0.01）。结论 p53可能通过诱导或促进细胞凋亡参与大鼠RIR;高血压状态下发生RIR视网膜毛细血管细胞凋亡更为严重,且尤以缺血再灌注后24 h为著。     

缺血性心肌病伴完全性左束支传导阻滞患者心室运动同步性的超声研究

目的：应用超声心动图技术比较孤立性左束支传导阻滞患者与缺血性心肌病（ICM）伴完全性左束支传导阻滞（CLBBB）患者左心室内、左右心室间收缩运动同步性的差异。方法：选择30例孤立性左束支传导阻滞患者纳入对照组。选择32例ICM伴CLBBB心衰患者纳入病例组。应用定量组织速度成像（QTVI）技术测定左室前后间隔、侧壁、前壁及下壁、后壁基底段及中间段共约12节段沿左室长轴运动的收缩期达峰值时间的标准差（Ts-SD）,M超测定胸骨旁长轴方向室间隔与左室后壁的时间延迟（SPWMD）,脉冲多普勒测定左右心室射血前时间差（IVMD）。结果：与对照组相比,病例组Ts-SD、SPWMD、IVMD明显增大（P〈0.001）。线性相关分析发现,左室内及左右心室间运动不同步与左室大小、QRS波宽度及N末端脑钠肽前体（NT-pro BNP）浓度呈正相关。结论：ICM伴CLBBB患者左心室内及左右心室间运动存在明显的不同步性。超声技术在评价心室运动不同步方面具有一定价值。     

5 020例健康体检人群幽门螺杆菌感染情况调查

通过雅安职业技术学院附属医院健康管理中心5 020例健康体检人群中幽门螺杆菌（Hp）感染状况,了解健康人群的Hp感染率及主要影响因素,提高本地区人群对Hp感染危害性的认识,并积极主动进行预防,最终达到有效预防Hp感染,降低其危害的目的。本次调查结果显示雅安市健康体检者中Hp的检出率为31.0%,年龄越大、文化程度低、无...