慢性阻塞性肺疾病患者对氧磷酶和氧化应激与全身炎性反应的研究

Objective To analyze the activity of paraoxonase (PON1) and explore the relationship of PON1 and oxidative stress with systemic inflammation response in the acute exacerbation phase and stationary phase in patients with chronic obstructive pulmonary disease (COPD). Methods Serum PON1 activity was measured by phenylacetate in 38 patients with COPD and 30 healthy people. The activity of glutathione peroxidase (GSH-Px) was detected by improved Hafeman method. Total antioxidant capacity (TAC) was measured by eolorimetry and malondialdehyde (MDA) level was measured by thiobarbituric acid colouration method. The interleukin-6 (IL-6) and interleukin-8 (IL-8) levels were detected by radioimmunoassay. The level of C-reactive protein (CRP) was measured by immune turbidimetry. Results In the acute exacerbation phase, the activity of serum PON1 was significantly lower in COPD group than in control group [(98.03±42.40)×103U/L vs. (136.00±60. 50)×103U/L, t=4.962, P<0.01], and it was negatively related to the IL-8 level (r= - 0. 589, P<0.01) and positively related to FEV1% (r= 0. 434, P<0. 05). The activity of GSH-Px was negatively related to the IL-6 level (r=-0. 362, P< 0. 05). In the stationary phase of COPD group, the activity of serum PON1 had no statistical difference compared with control group[(131.50±53.65))×103U/L vs. (136. 00±60.50)×103U/ L, t=2. 457, P>0. 053, and it was negatively related to the IL-8 level (r=-0. 563, P<0.05). Conclusions Serum PON1 activity is significantly decreased in acute exacerbation phase of COPD group compared with control group and it is positively related to FEV1%. The oxidative stress is closely related to systemic inflammation response in patients with acute exacerbation of COPD.     

慢性阻塞性肺疾病患者对氧磷酶和氧化应激与全身炎性反应的研究

Objective To analyze the activity of paraoxonase (PON1) and explore the relationship of PON1 and oxidative stress with systemic inflammation response in the acute exacerbation phase and stationary phase in patients with chronic obstructive pulmonary disease (COPD). Methods Serum PON1 activity was measured by phenylacetate in 38 patients with COPD and 30 healthy people. The activity of glutathione peroxidase (GSH-Px) was detected by improved Hafeman method. Total antioxidant capacity (TAC) was measured by eolorimetry and malondialdehyde (MDA) level was measured by thiobarbituric acid colouration method. The interleukin-6 (IL-6) and interleukin-8 (IL-8) levels were detected by radioimmunoassay. The level of C-reactive protein (CRP) was measured by immune turbidimetry. Results In the acute exacerbation phase, the activity of serum PON1 was significantly lower in COPD group than in control group [(98.03±42.40)×103U/L vs. (136.00±60. 50)×103U/L, t=4.962, P<0.01], and it was negatively related to the IL-8 level (r= - 0. 589, P<0.01) and positively related to FEV1% (r= 0. 434, P<0. 05). The activity of GSH-Px was negatively related to the IL-6 level (r=-0. 362, P< 0. 05). In the stationary phase of COPD group, the activity of serum PON1 had no statistical difference compared with control group[(131.50±53.65))×103U/L vs. (136. 00±60.50)×103U/ L, t=2. 457, P>0. 053, and it was negatively related to the IL-8 level (r=-0. 563, P<0.05). Conclusions Serum PON1 activity is significantly decreased in acute exacerbation phase of COPD group compared with control group and it is positively related to FEV1%. The oxidative stress is closely related to systemic inflammation response in patients with acute exacerbation of COPD.     

慢性阻塞性肺疾病患者对氧磷酶和氧化应激与全身炎性反应的研究

Objective To analyze the activity of paraoxonase (PON1) and explore the relationship of PON1 and oxidative stress with systemic inflammation response in the acute exacerbation phase and stationary phase in patients with chronic obstructive pulmonary disease (COPD). Methods Serum PON1 activity was measured by phenylacetate in 38 patients with COPD and 30 healthy people. The activity of glutathione peroxidase (GSH-Px) was detected by improved Hafeman method. Total antioxidant capacity (TAC) was measured by eolorimetry and malondialdehyde (MDA) level was measured by thiobarbituric acid colouration method. The interleukin-6 (IL-6) and interleukin-8 (IL-8) levels were detected by radioimmunoassay. The level of C-reactive protein (CRP) was measured by immune turbidimetry. Results In the acute exacerbation phase, the activity of serum PON1 was significantly lower in COPD group than in control group [(98.03±42.40)×103U/L vs. (136.00±60. 50)×103U/L, t=4.962, P<0.01], and it was negatively related to the IL-8 level (r= - 0. 589, P<0.01) and positively related to FEV1% (r= 0. 434, P<0. 05). The activity of GSH-Px was negatively related to the IL-6 level (r=-0. 362, P< 0. 05). In the stationary phase of COPD group, the activity of serum PON1 had no statistical difference compared with control group[(131.50±53.65))×103U/L vs. (136. 00±60.50)×103U/ L, t=2. 457, P>0. 053, and it was negatively related to the IL-8 level (r=-0. 563, P<0.05). Conclusions Serum PON1 activity is significantly decreased in acute exacerbation phase of COPD group compared with control group and it is positively related to FEV1%. The oxidative stress is closely related to systemic inflammation response in patients with acute exacerbation of COPD.     

热塑型垫形变时间精细化管理在放射治疗全流程中的应用

目的 配合放射治疗热塑型垫专用烤箱,选择合适的温度控制挡位,合理掌握热塑型垫升温软化及塑形冷却时间,估算放射治疗制模时间,缩短放射治疗全流程时间,实现精细化管理。方法 提前预热烤箱,并用红外激光测温枪确定箱内达到指定温度后,分组将热塑型垫放入70、75、80、85℃状态的烤箱中,加热1 h,期间每隔5 min取出,用红外激光测温枪分别测出热塑型垫4个角(A、B、C、D)及中心(E)的温度值,然后计算该时间段5个测温点的平均温度;加热至1 h后取出,每隔5 min重复上述测温步骤,直至热塑型垫冷却至室温;将70℃组设为对照组,其余3组设为观察组,记录4种温度状态下热塑型垫的升温软化时间和降温冷却时间,并将数据代入IBM SPSS 26.0统计软件且进行各温度组间的配对样本t检验。结果 4种温度状态下,热塑型垫升温软化至可以塑形的加热时间分别为30、20、15、15 min,降温冷却至室温的时间分别为15、15、25、25 min; 70℃与75℃组比较,差异无统计学意义(P>0.05);70℃与80℃组以及70℃与85℃组比较,差异均有统计学意义(P<0.05)。结论 4种烤...     

白藜芦醇影响肺腺癌患者癌组织细胞增殖及CXCR_4表达

目的:通过使用白藜芦醇作用于肺腺癌患者癌组织A549细胞,研究其对癌细胞细胞周期及趋化因子受体CXCR4的影响。方法:对使用白藜芦醇的处理的癌组织细胞应用噻唑蓝比色其对A549细胞周期的影响;免疫印迹法检测白藜芦醇对A549细胞CXCR4表达的影响。结果:白藜芦醇确可显著抑制肺腺癌A549细胞的增殖和CXCR4的表达,而且抑制效果都有时间和浓度依赖性。结论:白藜芦醇对人肺腺癌A549细胞的增殖和CXCR4的表达有明显的抑制作用,而且主要是通过抑制DNA合成前期G1期来实现的。     

支气管上皮中GULP1蛋白的表达与慢性阻塞性肺疾病严重程度的关系研究

目的探讨支气管上皮中GULP1蛋白的表达与慢性阻塞性肺疾病患者严重程度的关系。方法选取2015年1月-2016年1月期间我院收治的100例慢性阻塞性肺疾病患者作为研究对象,设为观察组,根据其疾病进展分为轻中度组(n=64)和重度组(n=36),选取同期非慢性阻塞性肺疾病患者作为对照组(n=50),比较不同组患者的GULP1蛋白表达阳性率,并对GULP1蛋白表达与慢阻肺分级以及肺泡巨噬细胞数量相关性进行分析。结果观察组患者支气管上皮中GULP1蛋白的表达阳性率(69.0%)显著高于对照组(2.0%)(P0.05);重度组患者的支气管上皮中GULP1蛋白的表达阳性率(97.2%)显著高于轻中度组(53.1%)(P0.05);Spearman相关性分析结果显示支气管上皮中GULP1蛋白的表达评分与慢阻肺分级呈显著正相关(r=0.145,P=0.003);Spearman相关性分析结果显示慢阻肺患者支气管上皮中GULP1蛋白的表达与肺泡巨噬细胞数量呈显著正相关(r=0.432,P=0.015)。结论慢阻肺疾病患者支气管上皮中GULP1蛋白的表达水平显著增加,且与疾病的严重程度呈正相关,GULP1蛋白参与慢阻肺疾病调控过程可能与肺泡巨噬细胞相关,值得进一步深入研究。     

巨噬细胞甘露糖受体与呼吸系统疾病研究进展

甘露糖受体是参与免疫反应的一种模式识别受体,可以调节巨噬细胞的活化,呈递抗原及吞噬等过程,甘露糖受体下调或缺失将会影响机体对病原体的易感性.本文对近年巨噬细胞表面甘露糖受体基本结构和分布以及甘露糖受体在呼吸系统相关疾病中的研究进行综述.     

癌组织中微小RNA-200c水平对非小细胞肺癌患者预后评估的价值

目的:探讨癌组织中micro RNA-200c表达水平对非小细胞肺癌(NSCLC)患者预后的预测价值。方法:选择接受肺叶切除、全肺切除联合系统性淋巴结清扫术治疗的NSCLC患者100例,均经病理确诊。根据癌组织中micro RNA-200c水平将患者分为micro RNA-200c低表达组(40例)和micro RNA-200c高表达组(60例),分析micro RNA-200c水平与临床、病理特征的关系,应用单因素、多因素非条件Cox回归分析影响NSCLC患者预后的危险因素。绘制Kaplan-Meier法生存曲线,分析NSCLC组织中micro RNA-200c表达水平与患者预后的关系。结果:micro RNA-200c在NSCLC组织中表达水平高于癌旁正常组织(7.65±2.01 vs 4.01±0.88,P<0.05)。NSCLC组织中micro RNA-200c表达水平与年龄、性别、吸烟史、嗜酒史、组织类型、分化程度、肿瘤直径无关(均P>0.05),与TNM分期、N分期有关(均P<0.05)。单因素、多因素Cox回归分析显示,N分期(N1~N2期)、micro RNA-200c高表达是影响NSCLC患者无进展生存率和总生存率的危险因素(均P<0.05)。Kaplan-Meier法生存曲线显示,NSCLC组织中micro RNA-200c高表达组患者无进展生存率、总生存率显著低于micro RNA-200c低表达组(均P<0.05)。结论:肺癌组织中micro RNA-200c高表达不利于NSCLC患者预后,其可作为评估NSCLC患者预后的分子标志物。     

慢性阻塞性肺疾病患者对氧磷酶和氧化应激与全身炎性反应的研究

目的 探讨慢性阻塞性肺疾病(COPD)患者在急性期与稳定期血清对氧磷酶(PoN1)的变化及其与氧化应激、全身炎性反应的关系. 方法 对38例COPD患者在急性期和稳定期血清的PON1活性采用乙酸苯酯法检测,改良Hafeman法检测血清谷胱甘肽过氧化物酶(GSH-Px)活性,比色法测定总抗氧化能力(TAC),硫代巴比妥酸显色法测定丙二醛(MDA)水平,放射免疫法测定血清白介素6(IL-6)和白介素-8(IL-8)水平,免疫投射比浊法测定血清C反应蛋白(CRP)含量. 结果 COPD急性期患者血清PON1活性(98.03±42.40)×103U/L显著低于健康对照组的(136.00±60.50)×103U/L(t=4.962,P<0.01),COPD急性期患者PONl活性与IL-8水平呈显著负相关(r=0.589,P<0.01),与FEV1%呈正相关(r=0.434,P<0.05);GSH-Px活性与IL-6水平呈负相关(r=-0.362,P<0.05);COPD稳定期患者血清PONl活性(131.50±53.65)×103U/L与健康对照组(136.00±60.50)×103U/L比较,差异无统计学意义(t=2.457,P0.05),GSH-Px活性与IL-8水平呈负相关(r=-0.563,P<0.05). 结论 COPD急性期患者PON1活性明显降低,与FEV1%存在正相关,且氧化应激与全身炎性反应之间密切相关.     

CAD/CAM氧化锆全瓷在口腔修复中的效果与价值探讨

目的分析研究CAD/CAM氧化锆全瓷在口腔修复中的应用效果与实际价值。方法选取我院口腔科2015年6月~2016年8月收治的80例口腔修复患者作为本次研究对象,根据治疗方法的不同分为观察组与对照组,每组40例。观察组患者实施CAD/CAM氧化锆全瓷修复治疗;对照组患者实施合金烤瓷修复治疗。对两组患者进行为期一年的跟踪随访,记录并对比两组患者的修复效果、治疗满意度。结果察组患者的修复效果显著优于对照组患者(P0.05),差异有统计学意义。观察组患者的治疗满意度为97.50%,对照组患者的治疗满意度为80.00%,差异有统计学意义(P0.05)。结论对需要口腔修复的患者实施CAD/CAM氧化锆全瓷修复治疗,可显著提高治疗的效果,降低不良事件的发生,且在美观、功能等方面满足患者的需求,患者满意度高,有利于构建和谐的医患关系,值得临床推广应用。