FGA-128C/G基因多态性与脑梗死的相关性及其对血浆纤维蛋白原的影响

目的 探讨中国湖南汉族人群FGA-128C/G基因多态性与脑梗死的关系及其对血浆纤维蛋白原(fibrinogen,Fg)的影响.方法 应用聚合酶链反应-限制性酶切片段长度多态性方法对中国湖南汉族194例脑梗死患者和114名与之年龄、性别等相匹配的健康体检者进行FGA-128C/G基因多态分析,并经基因测序证实.结果 在中国湖南汉族人群中,FGA-128C/G存在CC、CG基因型,未发现GG基因型;两种基因型和等位基因频率在脑梗死组和对照组之间比较差异无统计学意义(P>0.05);Fg水平在两种基因型之间比较差异亦无统计学意义(P>0.05);通过对血浆Fg影响因素的多元回归分析发现,Fg水平与FGA-128C/G基因型无关(P>0.05),而随年龄增加而升高,男性高于女性(回归系数分别为0.079,-0.534).结论 中国湖南汉族人群存在FGA-128C/G基因多态性;FGA-128C/G基因多态性与脑梗死患者血浆Fg水平增高无显著相关性,可能不是湖南汉族人群脑梗死的遗传易感基因;年龄和性别是湖南汉族人血浆Fg水平的主要影响因素.     

胸腔内脂肪肉瘤七次切除一例

患者男,57岁,因"右胸痛3 d"于2005年12月6日入院.查体:右下肺呼吸音减弱,余无异常.CT示右下胸腔巨大肿块,大小10.0 cm×11.0 cm×10.5 cm,呈囊实性,内见脂肪密度影,其内CT值13～18 HU,未见钙化;右下肺压缩、肺不张,周围胸膜增厚粘连;肺门及纵隔未见肿大淋巴结.     

自体组织代气管的动物实验研究

目的延长气管的切除长度。方法采用犬的自体带肌血管蒂的肋骨，弯曲缝制成环状后，间置在气管两切端，代替部分切除的气管，获得成功。结果间置的肋骨环与气管吻合处组织愈合良好，管腔通畅，气管粘膜爬入并覆盖肋骨环内壁。结论可延长气管切除长度２．５～４．０ｃｍ左右，较人造气管有独到优点，为气管代用材料的研究提供一种新方法。     

原发性食管非何杰金淋巴瘤1例

原发性食管非何杰金淋巴瘤１例蒋仲敏，王连生，孟凡利，刘培云，宋晓明，臧琦病人男，４２岁。进食梗噎４个月。查体：无异常发现。Ｘ线钡餐示食管中段管腔狭窄，粘膜破坏，可见小龛影，病变长约７ｃｍ。纤维镜检查距门齿３０ｃｍ处食管前后径明显狭窄，无法进镜，有鲜血...     

大黄属植物的外形、成分与泻下作用问联系性的多元分析

具有生理活性的药用植物,它们彼此在亲缘关系、化学成分和药理作用之间似有一定的内在联系。如能总结这些内在的规律,必将会对中草药的整理提高和新药寻找带来较大的方便。晚近得到飞跃发展的电子计算机技术以及某些多元统计方法,可以在这方面发挥巨大的作用。为了探索在这方面研究的基本方法,我们选择了种类并不太多、而且研究也较有基础的大黄属植物作为试点,应用多元统计和数量分类学的方法,对植物外形、成分和泻下作用等作进一步的     

PPARr2基因Pro12Ala多态性与脑梗死的关系

目的探讨过氧化物酶体增殖体激活受体γ2(PPAR-γ2)基因Pro12Ala多态性与脑梗死的相关性。方法用聚合酶链反应-限制性片段长度多态性方法分析190例脑梗死患者和200例正常对照人群PPARγ2Pro12Ala多态,并测定总胆固醇(TC)、空腹血糖、甘油三脂(TG)、血压、体重指数(BMI)、颈动脉内中膜厚度(IMT)等。结果脑梗死组和正常对照组PPARγ2基因P/P型频率分别为0.900和0.884,P/A型频率分别为0.095和0.116,PPARγ2基因频率分布在两组间无显著性差异(P>0.05)。脑梗死组中,P/A A/A型颈动脉IMT较P/P型显著增高(P<0.05)。结论PPARr基因Pro12Ala多态性可能不是脑梗死发病的遗传因素,但可能与动脉粥样硬化及颈动脉内中膜增厚有关。     

原发性气管肿瘤与大气道阻塞的外科治疗

采用手术及钛镍记忆合金支架治疗恶性肿瘤大气道阻塞28例。行气管袖式切除术10例,气管肿瘤局部切除术8例,肿瘤刮除 支架5例,单纯支架治疗5例。全组病例无围手术期死亡,5、10及>10年生存率分别为53·6%(15/28)、35·7%(10/28)和10·7%(3/28)。回顾性资料分析结果表明,早期诊断、积极手术或支架置入及综合治疗是治疗原发性气管肿瘤及大气道阻塞及提高生存率的有效手段。     

蛋氨酸合酶基因A275 6G位点多态性与非综合征型唇腭裂的关联性研究

Objective To study the association of the A2756G polymorphism of the methionine synthase (MS) gene with nonsyndromie cleft lip with or without cleft palate (NSCL/P) in Chinese. Methods Ninety-seven NSCL/P case-parent triads were selected as the case group. One hundred and four healthy subjects and their biological parents were selected as control group. For all subjects the A2756G polymorphism of the MS gene was examined by PCR-RFLP method. Results There was no statistical difference in genotype and allele frequencies for MS A2756G variants among family members between case group and control group. The GG genotype was not detected in the offsprings and mothers. The odds ratio and confidence interval of genotype AG in offspring, father and mother were 1.78(0.74-4.34), 0.80(0. 36-1.79) and 1.26(0. 54-2.93) respectively. The odds ratio and confidence interval of allele G in offspring, father and mother were 1.70(0.78-3.73), 0. 88(0. 49-1. 75) , and 1.23(0.59-2.60) respectively. The G allele did not increase the risk of NSCL/P. Transmission disequilibrium test (TDT) analysis yielded no evidence of linkage disequilibrium (χ2=0.034,P>0. 05). The results of haplotype-based haplotype relative risk (HHRR) analysis (χ2=0.03,P>0.05) and family-based association tests (FBAT) (Z=0. 186, P> 0.05) failed to show association between the MS A2756G variant and the risk of NSCL/P. Conclusion The A2756G polymorphism of the MS gene was not associated with NSCL/P in Chinese in the present study.     

蛋氨酸合酶基因A275 6G位点多态性与非综合征型唇腭裂的关联性研究

Objective To study the association of the A2756G polymorphism of the methionine synthase (MS) gene with nonsyndromie cleft lip with or without cleft palate (NSCL/P) in Chinese. Methods Ninety-seven NSCL/P case-parent triads were selected as the case group. One hundred and four healthy subjects and their biological parents were selected as control group. For all subjects the A2756G polymorphism of the MS gene was examined by PCR-RFLP method. Results There was no statistical difference in genotype and allele frequencies for MS A2756G variants among family members between case group and control group. The GG genotype was not detected in the offsprings and mothers. The odds ratio and confidence interval of genotype AG in offspring, father and mother were 1.78(0.74-4.34), 0.80(0. 36-1.79) and 1.26(0. 54-2.93) respectively. The odds ratio and confidence interval of allele G in offspring, father and mother were 1.70(0.78-3.73), 0. 88(0. 49-1. 75) , and 1.23(0.59-2.60) respectively. The G allele did not increase the risk of NSCL/P. Transmission disequilibrium test (TDT) analysis yielded no evidence of linkage disequilibrium (χ2=0.034,P>0. 05). The results of haplotype-based haplotype relative risk (HHRR) analysis (χ2=0.03,P>0.05) and family-based association tests (FBAT) (Z=0. 186, P> 0.05) failed to show association between the MS A2756G variant and the risk of NSCL/P. Conclusion The A2756G polymorphism of the MS gene was not associated with NSCL/P in Chinese in the present study.     

铁强化物中铁在人体吸收及维生素A、C中的作用

为测定还原铁强化玉米片中铁的吸收以及维生素A和维生素C对改善铁吸收的作用,研究了87名受试对象摄入由谷物、牛奶和糖组成的四餐食物,食物中添加了铁、不同浓度的维生素A和维生素C。采用放射性铁标记的方法,通过检测、计算受试对象血液中放射性铁化合物的量,以确定铁的吸收情况。结果表明,在食物中同时添加维生素A和维生素C两种维生素时,铁吸收率明显增加了3．6倍;体外溶解性试验显示,在pH为6时比pH为2时,维生素A和维生素C有促进氯化铁、电解质铁和还原铁溶解的重要作用;在即食谷物中添加维生素A和维生素C,能显著改善铁的吸收,部分归因于它们对还原铁的溶解性具有的一定作用。