碘过量和多聚肌苷酸-聚胞苷酸及甲状腺球蛋白诱发小鼠甲状腺炎对Toll样受体3表达的影响

Objective To observe the effect of iodine excess(HI),polyinosinic-polycytidylic acid[Poly(I:C),Poly]and thyroglobulin(TG)on the thyroid of mice by the expression of Toll-like receptor 3(TLR3)to reveal the functional role of TLR3 in autoimmune thyroiditis.Methods Forty-two non-obese diabetic mice,body weight (20±3)g,were divided into six groups:control group,HI group,Poly group,TG group,HI+TG group,HI+Poly group. Fed with deionized water and injected intraperitoneally with physiological saline 0.1 ml each day for a week, the mice in control group were injected with physiological saline every other day at the same dose for 1 week before they were sacrificed; HI group drank 0.05% NaI water and were injected intraperitoneally with physiological saline same as control group; Poly group drank deionized water and were injected intraperitoneally with poly 0.1 ml (1 g/L)each day of the week, then the mice were injected with Poly every other day at the same dose for 1 week before they were sacrificed; TG group drank deionized water and were injected intraperitoneally with physiological saline same as control group, immunized with 0.1 mg TG by subcutaneously injecting and the immunization was enhanced after they were fed half dose for 4 and 8 weeks separately. In HI + Poly group, the treatment was the same as HI group and Poly group; HI + TG group: the treatment was the same as HI group and TG group. Eight weeks later, mice were sacrificed and thyroids were taken to make frozen sections, Hematoxylin-Eosin (HE) staining was employed to observe the morphological change of the thyroids. The expression of TLR3 of thyroids was observed under fluorescence microscope after Immumofluorescence using TLR3 antibody and TR3-positive cells were analyzed in the thyroid density. Results HE staining showed thyroids of Poly group had no inflammation under microscope.There were different degrees of inflammatory cell infiltration in HI group and TG group. The inflammatory cell infiltration and the damage of follicular thyroid of HI + TG group and HI + Poly group were serious, and the degrees of inflammation were higher over "++". Thyroid follicular epithelial cell with TLR3 expression could be seen in Poly group and HI group, meanwhile, there were TLR3 strong positive inflammatory cells in HI group under fluorescent microscope. Using stereological analysis of TLR3-positive cell density in the thyroid, the difference between groups was statistically significant(F=7.870, P<0.01 ). TLR3-positive cell density in the thyroid of HI + Poly group was higher[ (9.287 ± 0.522)mm2] than control group[ (0.062 ± 0.025)mm2, P < 0.01] significantly, meanwhile, the density in HI + Poly group was higher than HI group [ (2.574 ± 0.257 )mm2] and Poly group[ (1.361 ± 0.148 )mm2, all P < 0.01]. The density in HI + TG group[ (4.843±0.405)mm2] was higher than HI group and TG group[(1.601 ±0.268)mm2, all P < 0.01 )]. Conclusions Excessive iodine and thyroglobulin can induce thyroiditis, and stimulate the expression of TLR3 in the thyroid follicular epithelial, Poly aggravated thyroiditis induced by iodine excess in NOD mice; TLR3 positive inflammatory cells also appeared in inflammatory region, suggesting that TLR3 is involved in the pathogenesis of autoimmune thyroiditis     

心房肽Ⅲ对离体大鼠心肌缺血再灌注后心律失常的影响

目的 观察心房肽Ⅲ对离体大鼠心肌缺血再灌注后心律失常的影响.方法 离体心脏停灌30 min,造成全心缺血,复灌60 min.设立6个组:正常对照组、缺血再灌注模型对照组、阳性对照组、APⅢ高剂量组、APⅢ中剂量组、APⅢ低剂量组.连续监测心电图,观察心律失常情况.结果 与缺血再灌注模型对照组比较,APⅢ可减少再灌...     

2005～2007年新疆手足口病流行病学特征分析

目的探讨新疆手足口病疫情流行特征,以便提出针对性控制对策。方法用描述性流行病学方法,对新疆2005～2007年通过网络直报的手足口病疫情进行分析。结果新疆手足口病发病率呈上升趋势、无死亡,多为散发,有明显的季节性高峰,5～8月为高发季节;发病以0-6岁儿童为主。结论手足口病疫情的发生危害了新疆儿童身心健康,对0-6岁儿童做到“早发现、早预防、早治疗”是有效控制传染病发生的关键。     

保留盆腔神经宫颈癌根治术与传统宫颈癌根治术的比较研究

目的：探讨保留盆腔自主神经的宫颈癌根治术与传统宫颈癌根治术对患者术后生活质量的影响及其手术效果的初步评估。方法：选择皖南医学院第一附属医院2011年1月至2012年09月收治的经国际妇产科学联盟（FIGO）分期为IB～ⅡA的子宫颈癌患者50例，设研究组（n=20）采用保留盆腔自主神经的宫颈癌根治术，对照组（n=30）行传统的宫颈癌根治术，比较两组手术范围、手术时间、术中出血量、导尿管保留天数、残余尿量、肛门排气、排便时间及术后半年性功能恢复情况。结果：对照组与研究组比较，术后第10天残余尿量分别为（201．83±83．00）、（139．75±69．01）mL，保留尿管天数（15．20±2．43）、（11.85±2．08）d；术后肛门排气时间分别为（72．97±16．31）、（59．60±13。37）h，术后肛门排便时间分别为（121．17±23．07）、（91．05±11．66）h，两组相比差异具有统计学意义（P〈0．05）；术后半年性功能随访结果显示：对照组性功能障碍的发生率为86．7％，研究组性功能障碍发生率为45％，两组相比差异有统计学意义（P〈0．05），对术后半年未恢复性生活的患者进行原因调查，发现因“害怕疾病复发”占77．8％；害怕“性交病或出血”占55．6％。研究组与对照组手术时间中位数分别为250（180～330）min和200（150～270）min，术中手术时间、出血量、术中切除主骶韧带、阴道长度及淋巴结清扫数目等指标差异均无统计学意义。结论：保留盆腔自主神经的宫颈癌根治术治疗早期宫颈癌是安全可行的，与传统根治术相比更有利于膀胱、直肠功能的恢复，有助于提高患者术后的生活质量。     

诱导期血液透析者的自我护理指导

慢性肾衰终末期由保守疗法向稳定的维持性透析过渡中的一段时间，称诱导期^[1]，这一阶段患者心理和身体均有很大变化，为确保血液透析的顺利进行，减少各种并发症的发生，对患者进行自我护理指导尤为重要。自2000年3月以来，我们对32例诱导期血液透析患者开展了自我护理指导，效果满意，现介绍如下。     

盐酸左氧氟沙星联合替硝唑治疗慢性盆腔炎的效果观察

目的：探讨盐酸左氧氟沙星(LH)联合替硝唑(TNZ)治疗慢性盆腔炎(CPI)的临床效果。方法：选取2020年7月—2021年10月山东省临沂市兰山区人民医院妇产科收治的CPI患者50例作为研究对象，采用随机数字表法分为对照组和观察组，各25例。对照组给予TNZ治疗，观察组在对照组基础上联合LH治疗。比较两组治疗效果。结果：治疗后，观察组红细胞沉降率、血浆黏度、高切全血黏度、低切全血黏度、C反应蛋白、白细胞、CD8+、盆腔积液深度低于对照组，CD4+高于对照组，差异有统计学意义(P<0.001)。观察组舒张期最低流速、阻力指数、搏动指数均高于对照组，差异有统计学意义(P<0.001)。结论：LH联合TNZ治疗CPI的效果确切，可有效改善患者血流动力学指标、炎性指标、淋巴细胞水平，降低盆腔积液深度。     

151 537例围生儿出生缺陷监测

为了连续、动态地观察围生儿出生缺陷的发生情况 ,准确掌握出生缺陷儿的区域分布、性别分布、发生顺位等情况 ,为卫生行政部门提供可靠的资料 ,以便找出干预措施 ,现就我市 1998年 1月至 2 0 0 1年 12月间 15 15 37例围生儿出生缺陷监测结果进行分析。对象与方法1.监测对象 :采用围生期 I的规定 ,对在监测医院住院分娩的孕满 2 8周至产后 7d内的围生儿 (包括活产、死胎、死产 )进行监测 [1 ] 。2 .资料来源 :青岛市承担围生儿出生缺陷监测任务的2 0所医院 1998年～ 2 0 0 1年上报的 15 15 37例围生儿情况月报表及出生缺陷登记卡。3.诊断标…     

青岛市1992～1995年集体儿童健康状况的动态观察

90年代国际儿科学界对儿童保健给予特殊的重视,还把提升儿童期生命质量和健康水平视为儿科及儿童保健领域内跨世纪研究的重要前沿.为赶上先进水平,我市4年来在抓集体儿童保健质量的同时,并动态观察全市集体儿童健康发展状况,对儿童体检中几个主要项目进行了调查研究.     

返魂草冲剂治疗慢性气管炎30例疗效观察

慢性气管炎是我国北方常见病多发病。尤其是黑龙江省位处寒冷的地区,发病率更高于其它地区,多年来虽然有些学者专家、教授不断研究,但是仍没特效药物能根治本病。我院自