利用Model ChemLab手段进行虚拟化学实验的研究

Model ChemLab采用独特的在同一个窗口内分划分不同区域的设计模式,在不同区域内兼有理论指导和现象观察两个交互式工作平台.一般采用常见的实验仪器来逐步模拟某一个实验进程,用户如同真实地完成了整个常见实验操作.Model ChemLab可以用于化学教学中的课前预习实验,对于特殊实验可以由实验教学人员根据具体需求通过升级工具、增设方案的方法,完成该特定的化学实验.     

氯解磷定引起高血压1例

氯解磷定引起血压明显升高在临床上少见,现将我院诊治的氯解磷定引起高血压1例报道如下：     

Cox7a2对睾酮合成及StAR、P450scc和3β-HSD蛋白表达影响研究

目的研究Cox7a2在TM3小鼠睾丸Leyidg细胞中过农达对LH诱导的睾酮合成及对StAR(睾酮合成调节关键蛋白),P450scc(胆同醇侧链裂解酶),3β—HSD(3β-羟基甾脱氢酶)蛋白表达的影响。方法构建Cox7a2荧光表达载体,转染TM3小鼠睾丸Leydig细胞,融合蛋白表达及LH诱导刺激后,ELISA测定细胞上清液中睾酮含量,Western blot检测StAR蛋白、P450scc和3β-HSD酶的表达变化。结果Cox7a2在TM3小鼠睾丸Leydig细胞中过表达抑制LH诱导的睾酮合成,降低StAR蛋白的表达,对P450scc和3β-HSD的蛋白表达没有明显影响。结论在TM3小鼠睾丸Leydig细胞中,Cox7a2至少通过抑制类固醇快速调节蛋白StAR的表达,进而抑制LH诱导的睾酮合成。     

雄激素剥夺条件下阻断自噬增加LNCaP细胞凋亡

Objective To investigate the change of apoptosis in LNCaP cells after inhibition of autophagic process under androgen removal conditions. Methods The autophagic level was deter-mined by using confocal microscopy and RT-PCR. The DAPI staining was used to indicate the apopto-sis of LNCaP cells after inhibition of autophagic by 3-MA. Also, Z-VAD-FMK was used to extend the apoptosis results. Results ①Androgen deprivation led to increased autophagy in LNCaP cells. LN-CaP cells cultured in complete medium(CM) presented low autophagic process with 1.9 scores. After 24 hours, the punetate GFP-LC3 structures were accumulated in the cells cultured in serum-free medi-um (SF)(2.64 scores). In contrast, the number of punctate GFP-LC3 remained at a very low level (1.85 scores), when cells were incubated with DHT in SFA(serum-free medium+DHT). Statistical analysis showed the significant difference between SF and SFA (P<0.01). Semiquantitative RT-PCR was employed to examine the mRNA expression of LC3. Indeed, cells grown in the medium without serum had a higher LC3 mRNA expression with the highest at 12 hour time point as compared with the cells grown in CM. DHT treatment reduced the level of LC3 mRNA. ②Blockage of autophagy by 3-MA increased the apoptosis of LNCaP cells. LNCaP cells in SF and SFA just presented a basal level of apoptosis, which is (3.19±1.09)% and (3.01±0.33)% , respectively. Under androgen-free con-ditions, inhibition of autophagy by 3-MA could increase apoptosis significantly(10. 90±2.91%). While Z-VAD-FMK, a pan Caspase inhibitor, was able to suppress this apoptotic process to the level of (1.16±0.52)%, which was statistically significant(P<0.01). Conclusions Androgen removal can lead to the increase of autophagy in LNCaP cells. Moreover, inhibition of autophagy promotes the occurrence of apoptosis.     

17城市妇幼保健院发表学术论文的统计分析

目的:了解山东省17城市妇幼保健院学术研究状况。方法:使用文献计量学方法收集数据并进行统计学处理。结果:山东省17城市妇幼保健院在各种期刊上共发表并被VIPD收录的论文数为1 759篇,呈逐年递增;其中创新性论著占39.51%,核心期刊发文量占21.60%,临床学科论文占46.79%,最高产作者著文25篇,平均作者合作度为2.34,合作率达78.68%。结论:山东省妇幼保健机构学术研究成绩显著。     

244例精神分裂症再入院的临床资料分析

２４４例精神分裂症再入院的临床资料分析湖北省东风汽车公司茅箭医院（４４２０１２）姜学军，李新胜在１９８２～１９９１年之间，我院共收治１６００例精神病人，其中再次入院并诊断为精神分裂症者共２４４例。现将其临床资料作如下分析。１一般资料本文共２４４例。其...     

生物医学期刊文献标引现状分析

文献标引,简称标引。它是针对文献的主要内容及某些外表特征,赋予文献检索标识的过程,是建立检索工具和检索系统的主要工作。为了了解国内生物医学期刊文献标引现状,对2008—04山东省医科院图书馆所订387种国内生物医学期刊中图分类号、关键词、文献标识码3项标引进行统计,并就其作用及在使用中发现的问题加以分析,以引起人们对文献标引工作的重视。