A genome-wide allelotype study of primary and corresponding recurrent glioblastoma multiforme in one patient

Glioblastoma multiforme (GBM) is the most common type of primary malignant brain tumor. Although comprehensive therapeutic measures are available, recurrence is very frequent and the prognosis of GBM remains dismal. To date, little is known about the molecular pathogenesis associated with GBM recurrence. According to Knudson ' s two-hit hypothesis of tumor suppressor gene (TSG) inactivation,1 deletion of a chromosomal region, as revealed by loss of heterozygosity (LOH), is often indicative of the presence of a potential TSG. Allelotype studies involving a comprehensive LOH analysis of the whole genome can provide more detailed and thorough information for detecting genetic anomalies than traditional LOH analysis. The present study is designed to conduct a genome-wide allelotype analysis of one patient ' s primary and corresponding recurrent GBM tumors in an effort to reveal molecular genetic alterations associated with the recurrence of this malignancy.     

341例AIDS合并肺结核患者痰液两种方法培养及其药敏分析

目的：探讨AIDS（艾滋病）合并肺结核患者痰液两种方法培养及其耐药情况。方法采集患者痰液分别采用BacT/ALERT3D系统和罗氏法进行结核分枝杆菌的培养及药物敏感性测定。结果341例AIDS合并肺结核患者痰液两种方法检测,共129例培养阳性,总阳性率39.81%,3D法培养污染率为2.6%,罗氏法培养污染率为2.3%。129株菌株药敏测定结果有40株出现耐药,耐药率为31.01%。结论3D法在AIDS合并肺结核患者痰液培养方面较罗氏法阳性率高,两种方法相互结合更能提高培养效率。AIDS合并肺结核患者痰液结核菌培养和药敏实验可为临床诊断和指导用药提供重要依据。     

FISH方法对X射线工作者受照剂量重建的研究

辐射流行病学调查结果表明低剂量电离辐射作用于人体引起人群肿瘤发病率和死亡率与接受的累积剂量有着密切相关性^[1].我国50年代和60年代X射线工作者使用防护条件较差的老机器, 这些人员受到的照射剂量较大, 因此进行剂量重建以观察远后效应是有意义的。     

臧堃堂运用玉屏风散的经验

玉屏风散由黄芪、白术、防风三味药以鼎足之势组成。南方医科大学臧堃堂教授在临床中多用玉屏风散加减治疗各种外感和内伤疾病,包括免疫过敏性疾病,疗效显著。其经验主要可归纳为:虚人外感,益气解表;益气强身,不拘外感;扶正抗敏,固卫祛邪。附验案1则以佐证。     

小儿服用蜂蜜误解之辨析

对于儿科临床医生来讲现实生活及临床治疗中对蜂蜜都存在一些误解和误判,认为蜂蜜中含有很多不利于儿童生长的成分,给大多数家长和和儿童造成了不必要的担忧和不利影响。针对此种情况,笔者从蜂蜜的一些常识及个人临证经验做一普及和辨析旨在造福大众。     

加强岗前培训、提升医疗服务质量

通过对五年来400名新上岗医护人员进行岗前培训,跟踪调查分析400名新聘医护人员的学习工作经历,调查结果显示住院病人满意度提高9%,医疗护理差错及纠纷降低15%.因此,加强岗前培训对促进医护人员角色转换,规范医疗服务行为,增强自我保护意识,防止医疗护理差错及纠纷,提升医疗护理服务质量和病人满意度起到了积极重要的作用.