自体半腱肌双股重建治疗膝关节内侧副韧带陈旧性损伤疗效观察

目的：观察应用自体半腱肌双股重建治疗膝关节内侧副韧带陈旧性损伤的手术疗效。方法选择23例膝关节内侧副韧带陈旧性Ⅱ～Ⅲ度损伤患者,采用自体半腱肌双股重建膝内侧副韧带,按照Lysholm膝关节评分评价疗效。结果23例均获得10个月以上的随访,患者均无膝关节内侧不稳的临床症状,23膝外翻应力试验1例弱阳性,术后外翻应力位X线片均＜3 mm,优良率达91．30％,Lysholm膝关节评分由术前的（43．6±3．4）分提高至术后6个月的（91．1±2．5）分（P＜0．05）。结论采用自体半腱肌双股重建治疗膝关节内侧副韧带接近解剖重建,术后膝关节功能可获得良好的恢复。     

肝脏移植术后发热、皮疹并腹泻

病历摘要 患者男,62岁.因肝炎后肝硬化、终末期肝病行同种异体改良背驮式肝移植术.术中应用甲基强地松龙(MP)1 000 mg,术后MP常规递减直至改口服强的松;术后第2天开始应用FK506,并根据血药浓度调整剂量.     

梗阻性黄疸对肝脏血流动力学的影响

目的探讨梗阻性黄疸对肝脏血流动力学的影响．方法采用彩色多普勒血流显象仪对２０例梗阻性黄疸患者的肝固有动脉和门静脉血流进行定量测量，并与２０例正常人进行对比分析．结果梗阻性黄疸患者肝固有动脉的峰值流速（ｃｍ／ｓ，９２±１５ｖｓ６９±１９，Ｐ＜００５）、血流量（ｍＬ／ｍｉｎ，７１４±３６３ｖｓ３０５±１２１，Ｐ＜００１）显著高于正常人；门静脉血管内径（ｃｍ，１２５±０２４ｖｓ１０４±０１９，Ｐ＜００１）及门脉充血指数（ｃｍ×ｓ，０１４１±００１３ｖｓ０１１９±００１３，Ｐ＜００１）明显高于正常人．但门静脉血流量及峰值流速则低于正常人．结论彩色多普勒血流显象仪探讨梗阻性黄疸时肝脏血流动力学改变的较理想非创伤性方法，根据其有效循环血容量的改变可初步判断梗阻性黄疸的预后     

纳米非晶金刚石膜对镍铬合金烤瓷修复体弯曲强度的影响

目的:探讨纳米非晶金刚石膜对镍铬合金烤瓷修复体弯曲强度的影响.方法:36个镍铬合金烤瓷试件随机分为6组(n=6),其中5组的整个试件的表面分别镀覆非晶金刚石膜厚为20nm、40nm、60nm、80nm、100nm,对照组不镀膜;按照ISO9693-1标准的三点弯曲法测试试件的弯曲强度.结果:镀膜后镍铬合金烤瓷试件的弯曲强度分别是:20nm组为60.84 ±6.75MPa、40nm组为79.16±9.82MPa、60nm组为86.23±11.43MPa、80nm组为81.40±9.69MPa、100nm组为64.56±7.07MPa,未镀膜组为46.15±5.53MPa.结论:镍铬合金烤瓷复合体的整个试件经镀覆纳米非晶金刚石膜后其弯曲强度显著增加;镀膜时选择40-80nm的膜厚可以获得较高的弯曲强度.     

老年经腹直肠癌手术应用快速康复外科的临床研究

目的：探讨快速康复外科（FTS）在老年直肠癌患者围手术期的临床效果。 方法：将78例老年直肠癌患者随机分成FTS组和对照组,比较两组术后排气（便）时间、住院时间及费用、并发症发生率、以及白细胞（WBC）、白蛋白（ALB）、C反应蛋白（CRP）等临床指标。 结果：FTS组患者术后排气（便）时间、术后住院时间、费用明显少于对照组（均P<0.05）,而术后并发症的发生并未增加（P>0.05）,FTS组患者术后第1,3天WBC、CRP低于对照组,而ALB明显高于对照组（均P<0.05）。 结论：在老年直肠癌切除术中应用FTS在保证不增加并发症的前提下,可促进肠道功能更快恢复,减轻应激及炎性反应,加快术后康复。     

胃空肠Roux-en-Y吻合术后吻合口-横结肠瘘一例

胃空肠Roux-en-Y吻合术后发生吻合口-横结肠瘘非常罕见,患者早期症状不明显且容易漏诊和误诊,典型三联征包括腹泻、体重减轻、粪性呕吐或粪性嗳气,确诊方式主要依据消化道造影和胃肠镜检查,对于明确诊断的患者需在营养状况改善的前提下尽早行手术治疗。本例患者通过行上消化造影及胃镜检查确诊该疾病,经过积极的术前准备和一期手术治疗后,症状明显改善。现将本中心对该疾病的诊疗过程及治疗体会报道如下。     

胆囊癌组织及胆汁中厌氧菌谱的检测

我们对1996年2月～1998年4月间我院收治的29例胆囊癌患者胆汁及胆囊癌组织行厌氧菌培养,现将结果报道如下。一、对象与方法1.研究对象及仪器:胆囊癌行手术患者29例,其中男11例,女18例,年龄19～63岁(平均38.5岁),其中行“Ｕ”型管引流术11例,行左右肝管引流术6例,行胆囊癌根治术9例,行胆囊造瘘术3例。实验仪器主要包括ｂａｃｔ/ａｌｅｒｔ120微生物自动检测系统和ｓｃｅｐｔｏｒ微生物鉴定系统。2.标本采集:术中用一次性无菌注射器穿刺病变胆囊,取胆汁5～10ｍｌ,1ｍｉｎ内注入厌氧增菌瓶,然后放入恒温振荡培养箱中自动培养16ｄ,底部感应器显…     

胆囊结石胆汁需氧菌厌氧菌及L型菌的培养鉴定

对５９例结石性胆囊炎患者胆汁同时进行需氧菌、厌氧菌和Ｌ型细菌的培养，分离鉴定和药敏试验。结果：５７例培养阳性，共检出细菌１１１株，其中需氧菌２６株、厌氧菌３株、Ｌ型细菌株；且以两各皮上细菌的混合感染为主。总检出率９４．９％。表明胆吉石患者同时做需氧菌、厌氮菌和Ｌ型细菌的培养能显著提高高原菌的检出率。药敏试验表明它们和自的抗菌谱不同，３种细菌同时培养对临床准确、合理选择抗菌药物有重要意义。     

Transwell侵袭实验测定M受体对胆管癌细胞侵袭的影响

Objective To explore the experiment condition and method for the application of in vitro in vasive Transwell chamber and to observe muscarinicreceptor stimulant and muscarinicreceptor antagonist's influence to cholangiocarcinoma's invasiveness.Methods Two hundred microliter cell suspension of various concentrations(0.5×105/mL,1.0×105/mL,1.5×105/mL and 2.0×105/mL)was added into the upper chamber of the Transwell chamber,and the cells were allowed to penetrate the matrigel for 12,18,24and 48 hours respectively.The numbers was gotten as the invasive cells on the under surface of the membrane.After optimal cell concentration and time were gotten,pilocarpine of various concentrations(0 mmol/L,0.1 mmol/L,0.3 mmol/L and 0.5 mmoL/L)was added into the upper chamber of the Transwell chamber,then the cells on the matrigel were stained and counted.So did the cells when atropine of various concentrations(0.01 mmol/L,0.01 mmol/L,0.05 mmoVL and 0.1 mmol/L)were added into the upper chamher of the Transwell chamber in according to pilocarpine of various concentrations(0 mmol/L,0.3 mmol/L,0.3 mmol/L and 0.3mmol/L).Results With the increase of the time and cell concentrations，the cells couts that penetrated the matrigel increased，while the increase tended to he stable when the culture time exceeded 36 hous and the cell concentration Was over 1.0×105/mL.By adding pilocarpine,there were significant differences between the control and experimental groups(P＜0.05)，but there were no significant differences in experimental groups with various concentrations.There were no significant differences in blank group and experimental groups with atropine added(P＞0.05).When added pilocarpine and atropine,there were significant differences between blank and experimental groups(P＜0.05)，but there were no significant differences in experimental groups with various concentrations.Conclusions Thirty-six hours as invasive time,and one cell concentration 1.0 × 105/mL were optimal to test invasion abilities of cholangiocarcingma cells to different medicines or reagents.There is the possibility that museariniereceptor exists in cholangiocarcinoma cells，and may play an important role in cholangiocarcinoma's invasiveness and metastasis.