E-cadherin基因启动子甲基化在恶性肿瘤浸润转移中的研究进展

远处转移是导致癌症患者死亡的最主要原因,明确调控肿瘤转移的分子机制成为控制和治疗肿瘤转移的核心环节。E-cadherin属于钙黏蛋白家族中的一员,通过参与钙离子依赖性细胞黏附力的调节而维持细胞间正常连接和保持上皮细胞极性。E-cadherin表达减少或缺失是导致肿瘤细胞发生侵袭和转移的重要因素,也是乳腺癌、肺癌、食管癌等上皮性肿瘤恶变的重要特征[1]。     

关木通中毒致急性肾功能衰竭一例

患者男,36岁。因恶心、呕吐、腰痛、少尿1d入住齐鲁医院。患者4d前因带状疱疹就诊于当地诊所,给予中草药等水煎口服,每日1副,其中每副含关木通10g。患者服用4副后出现恶心、呕吐,腰部疼痛难忍,尿少,尿量少于400ml／24h。到当地医院就诊,血常规正常,尿常规示pH6．5,     

改良腹腔镜下腹膜代阴道成形术在先天性无阴道中的应用术

目的 探讨改良腹腔镜下腹膜代阴道成形术在先天性无阴道患者中的应用及疗效.方法 2008年8月～2011年11月对29例患者应用腹腔镜腹膜代阴道成形手术,且采用始基子宫及阴道板使阴道顶端加固.结果 29例手术均顺利完成.手术时间80 ～ 120m in,平均90 min;术中出血量30 ～100mL,平均60mL;术后住院时间5～8d,平均6.2 d;无任何手术并发症发生;患者均术后随访:外阴恢复正常形态,阴道壁光滑,黏膜粉红,湿润,弹性良好,无异味产生及性交不适.结论 改良腹腔镜下腹膜代阴道成形术微创、术后恢复时间短、效果满意,值得推广.     

产前超声筛查在胎儿手部畸形中的应用价值

目的探讨产前超声在筛查胎儿手部畸形中的应用价值。方法回顾总结我院检出的手部畸形胎儿的相关资料。结果本组共发现胎儿手部畸形25例,其中手腕部姿势异常5例,手(指)缺失8例,先天性多(并)指12例。产前超声检出15例,漏诊10例,检出率60%。孕18～24周和孕25～32周胎儿手部畸形检出率较高。结论产前超声筛查可提高胎儿手部畸形的检出率,对胎儿产前畸形的筛查有重要价值。     

肾移植急性排斥反应中内皮素与细胞增殖变化的关系

内皮素（ＥＴ）是一种可溶性生物活性物质，其可通过特殊受体调节肾系膜细胞收缩或增生，系膜细胞的增殖变化可使肾小球的代偿发生改变，这种变化的结果，造成了一部分肾小球的损害，从而引起肾功能衰竭。我们通过检测肾移植患者急性排斥反应中的ＥＴ和增殖细胞核抗原（Ｐ...     

下丘脑褪黑素受体亚型基因及蛋白表达的研究

目的：应用PT－PCR技术和免疫组织化学染色显示人胚胎下捕脑褪黑素受体及其亚型的分布。方法：取人胚胎下丘脑组织，抽提总RNA并应用RT－PCR方法检测其mRNA，扩增所得阳性产物用自动测序仪测序；石蜡包埋下丘脑组织，应用鼠抗人mt1和MT2受体单克隆抗体分别检测褪黑素mt1和MT2受体亚型mRNA及其蛋白的表达。结果和结论：人胚胎下丘脑组织中存在mt1和MT2受体亚型mRNA的表达及其蛋白的翻译，提示在该组织中有mt1和MT2两种受体亚型的表达。     

筛窦脑膜瘤CT检查一例报告

筛窦脑膜瘤ＣＴ检查一例报告刘士远，施增儒，刘会敏关键词筛窦；脑膜瘤１临床资料患者，女，９岁，自幼双眼不对称，近１年来左眼突出日渐明显，经常严重鼻衄３个月，双眼视力及眼球活动如常，无头痛呕吐，既往及家族史无特殊。体检：神经系统检查无阳性发现，外鼻无畸形...     

胃息肉160例报告

在胃肠道息肉中,胃息肉较少见。我院胃镜室自1977年7月至1986年6月经胃镜检查诊断为胃息肉者160例。本文就其临床及病理资料进行回顾性分析,并对活检与全息肉标本的组织学检查结果进行比较以探讨其诊断意义。     

Difference in microvascular structure between benign and malignant pulmonary nodules and its relationship with CT enhancement

Objective: To investigate the enhancement basis and the mechanisms of solitary pulmonary nodules (SPNs) by comparing the differences in microvascular structure between benign and malignant lesions. Methods: Dynamic contrast-enhanced CT scan was performed on 53 patients with SPNs (diameter<3 cm. 38 peripheral lung cancers, 5 hamartomas, 10 inflammatory lesions) using a Siemens Plus S or a Marconi MX8000 multi-slices spiral CT scanner. The time-attenuation curves were interpreted. The microvascular density (MVD) and the continuity of the microvessels' basemental membrane in the dissected specimens were observed with the ABC (avidin-biotin-complex) immuno-histochemical method in all patients. ResultS:The CT enhancement values of lung cancer (49. 05±16. 08 HU) and inflammatory lesions (49. 59±21. 30 HU) were significantly higher than those of hamartoma (8. 98±4. 56 HU) (t = 7. 48. P<0. 05; t = 8. 35, P<0. 05). But the enhancement of lung cancer was similar to that of inflammatory lesions (t = 0. 76. P>0. 05). The time-attenuation curve of inflammatory lesions tended to increase faster and reached a higher peak compared to the lung cancer, and both of them maintained a high plateau after crossing. The hamartoma showed a slight increase in the time-attenuation curve and demonstrated a low-plateau curve. The MVD of SPNs was positively correlated with CT enhancement (r=0. 8051). The microvascular counts of peripheral lung cancer (48. 45±10. 09) and inflammatory lesions (19. 60±19. 94) were significantly higher than those of hamartoma (8.70±7.30) (t = 11.64, P<0.001;t = 6. 09. P< 0. 001). but no significant difference was found between lung cancer and inflammatory lesions (t= -0. 26, P = (). 799). There was no difference in the continuity of basement membrane between nodules with an enhancement less than 30 HU and those with an enhancement higher than 30HU (x2 = 3. 13, P>0. 05 ). Conclusion: The microvascular counts mainly contribute to the enhancement value of SPNs. The basement membrane is not related to nodule enhancement, but it might influence the pattern of the time-attenuation curve.