我国首个白血病病毒血液筛查法通过鉴定

本刊讯日前,在北京召开的中国安全注射联盟会议会上获悉,目前,滥用注射、过度注射、重复使用一次性注射器等非安全注射行为已成为严重的公共卫生问题,并引起我国有关方面的高度重视。据卫生部医政司护理处处长郭燕红介绍,安全注射包括对病人的安全、对医务人员的安全和对环境与公众的安全,主要涉及注射用具与注射药物、注射的技术操作过程、注射后的用具处理等环节。当前,全国各地医疗机构尤其是县级以下的基层医院、医务所普遍存在以下非安全注射行为:注射器消毒达不到标准;一次性注射器反复使用;伪劣注射器用于临床;注射操作不当造成创伤…     

皮肤血管瘤组织中p15基因产物表达及意义

为探讨 p15基因在皮肤血管瘤组织中的表达情况及其临床病理学意义 ,采用免疫组织化学方法检测了 p15基因在 46例皮肤血管瘤组织 (其中增生期 2 0例 ,退化期 2 6例 )和 5例正常皮肤组织标本中的表达。结果显示 ,p15基因在退化期血管瘤内皮细胞表达水平高于增生期 ,差异有显著性 (P<0 .0 1)。     

皮肤血管瘤内皮细胞增殖和凋亡的研究

目的　探讨细胞增殖和凋亡状态与皮肤血管瘤发生和发展的机制。方法　采用免疫组织化学方法和末端脱氧核苷酸转移酶介导的末端标记技术 (TUNEL) ,检测 40例不同时期婴幼儿皮肤血管瘤及 2 0例周围正常皮肤组织内皮细胞增殖和凋亡状态。结果　 2 0例增生期皮肤血管瘤、2 0例退化期皮肤血管瘤及 2 0例正常皮肤组织平均增殖指数分别为 60 .5 8、1.47及 1.49,经配对t检验差异具有高度显著性 (P <0 .0 0 1)。 2 0例增生期皮肤血管瘤、2 0例退化期皮肤血管瘤及2 0例正常皮肤组织平均平均凋亡指数分别为 0 .0 97、1.0 2 1及 1.0 46,经配对t检验差别具有高度显著性 (P <0 .0 0 1)。结论　皮肤血管瘤的血管内皮细胞异常增生和凋亡在血管瘤的发生、发展和退化过程中起着重要作用 ,为临床治疗血管瘤提供新的思路。     

肾病综合征合并假性Bartter综合征1例报告

肾病综合征合并假性Bartter综征合尚未见报道,现报告1例如下。病例资料患者女,30岁,农民,已婚。因双下肢水肿,乏力,腹胀,少尿3个月,于1990年8月30入院。否认使用利尿剂史。既往体健.家族史无特殊。查体:血压17/11kPa,颜面及双下肢水肿,心肺(-)。主要实验室检查:尿蛋白6.5g/24h,尿钾     

相控型聚焦超声双焦点模式声场测量及分析

主要介绍了相控型聚焦超声双焦点模式下声场理论的仿真和实验测量．结果表明测量与仿真理论值相符。     

皮肤血管瘤内皮细胞增殖和凋亡指数比较

目的 :探讨细胞增殖和凋亡状态与皮肤血管瘤发生和发展的机制。方法 :依据Mulliken的分类标准收集临床手术切除的皮肤血管瘤标本 4 0例及皮肤血管瘤周围正常皮肤组织 2 0例 ,采用免疫组织化学方法和末端脱氧核苷酸转移酶介导的末端标记技术 (TdT mediateddUTPnickendlabelingTUNEL法 ) ,检测 4 0例不同时期婴幼儿皮肤血管瘤及 2 0例周围正常皮肤组织内皮细胞增殖和凋亡状态 ,并对凋亡和增殖细胞的阳性细胞数进行计数。结果 :4 0例皮肤血管瘤标本中有增生期 2 0例 ,退化期 2 0例。 2 0例增生期皮肤血管瘤平均增殖指数、平均凋亡指数及平均凋亡指数与平均增殖指数之比与 2 0例退化期皮肤血管瘤平均增殖指数、平均凋亡指数及平均凋亡指数与平均增殖指数之比比较 ,经配对t检验差别具有高度显著性 (P <0 .0 0 1)。增生期皮肤血管瘤平均增殖指数、平均凋亡指数及平均凋亡指数与平均增殖指数之比与正常皮肤血管瘤平均增殖指数、平均凋亡指数及平均凋亡指数与平均增殖指数之比比较 ,经配对t检验差别具有高度显著性 (P <0 .0 0 1)。结论 :血管瘤的血管内皮细胞异常增生和凋亡在血管瘤的发生、发展和退化过程中起着重要作用 ,为进一步研究和探讨血管瘤发生、发展和退化的病理机制及治疗提供理论依据     

心理护理干预对宫颈癌患者心理健康状况的影响

目的调查心理护理干预对宫颈癌住院患者的心理健康状况的影响,以期建立一套系统的临床护理干预措施指导临床实践。方法采用前瞻性随机对照研究方法,选取40例符合病例入选标准的宫颈癌患者,分为实验组和对照组,每组20例。对照组采用常规护理模式,实验组在常规护理模式的基础上实施个性化、多样化的心理护理,应用焦虑自评量表(SAS)和抑郁自评量表(SDS)评估干预前后两组患者心理健康状况。结果心理护理干预后实验组患者的SAS、SDS评分均显著低于对照组(P<0.05)。结论心理护理干预能够有效调整患者的心理健康状况,改善患者生存质量,提高治疗的有效性,具有临床推广应用价值。     

卵巢移位术对宫颈癌术后放疗卵巢功能影响的临床研究

目的:研究卵巢移位术对宫颈癌术后放疗卵巢功能的影响。方法:回顾性分析2006年2月至2009年2月49例行宫颈癌根治+卵巢移位术患者。根据术后病理检查,24例有高危因素(研究组),术后3～4周放疗,25例患者无高危因素未放疗(对照组),对两组患者进行随访,并通过微粒子化学发光法测定患者术前、术后放疗后、术后6月、术后1年、术后2年的性激素(E2、P、FSH、LH)值及调查患者是否出现围绝经期症状。对两组患者进行对比,探讨放疗对移位卵巢功能的影响。结果:研究组20例2年后激素仍在生育期水平,患者无围绝经期症状;4例出现围绝经期症状,其中3例患者卵巢储备功能下降,1例卵巢功能衰竭。对照组22例2年后激素处于生育期水平,无围绝经期症状;3例出现围绝经期综合症,其中1例卵巢储备功能下降,2例卵巢功能衰竭。结论:宫颈癌根治中卵巢移位至髂棘上四横指同侧结肠旁沟内可能明显减小放疗对卵巢功能的损害。     

Angiogenic Effect of Intercellular Adhesion Molecule-1

In order to investigate the angiogenic effect of intercellular adhesion molecule-1 (ICAM-1), two parts of experiment were performed. Chick embryo chorioallantoic membrane (CAM) assay was used for in vivo angiogenic research. The chick embryos were divided into 4 groups: ICAM-1 group (divided into 3 subgroups, Ⅰ, Ⅱ and Ⅲ) for screening the angiogenic effect of ICAM-1 by adding different concentrations of ICAM-1 (0.1, 0.2 and 0.3 μg/μL) 5 μL into the chick embryo CAMs on the day 10 after incubation for every subgroup; Anti-ICAM-1 group A (divided into 2 subgroups, Ⅰ and Ⅱ) by adding different concentrations of Anti-ICAM-1 (1:100, 1:50) 5μL into the chick embryo CAMs on the day 10 after incubation for every subgroup to evaluate the effect of ICAM-1 on the survival of microvessels through observing whether Anti-ICAM-1 could induce involution of the microvessels on CAMs; Anti-ICAM-1 group B (divided into 2 subgroups, Ⅰ and Ⅱ) by adding different concentrations of Anti-ICAM-1 (1:100, 1:50) 5 μL into the chick embryo CAMs on the day 6 after incubation for every subgroup to evaluate whether ICAM-1 involved in embryonic angiogenesis through observing the growth of microvessels on CAMs; Control group: ICAM-1 or Anti-ICAM-1 was substituted by PBS 5 μL on the day 10 or day 6 after incubation. Three days later, the CAMs were photographed in vivo, excised, sectioned and the number of microvessels was counted. In ICAM-1 group, there was increased number of microvessels arranged radially with "spoked-wheel" pattern around the gelatin sponges. The new microvessels growing perpendicularly to gelatin sponges were observed. The number of the microvessels growing in the CAM mesen-chymes around the sponges in 3 subgroups was higher than that in control group (P<0.01), however, there was no significant difference among the 3 subgroups (P>0.05). In anti-ICAM-1 group A, the radially arranged microvessels were very unclear around the sponges contrast to that of ICAM-1 group. Few new microvessels were detected in the center of the sponges. The number of the microvessels growing in the CAM mesenchymes around the sponges in subgroup Ⅱ was lower than that in control group (P<0.01). There was no significant difference in the number of the microvessels around the sponges between subgroup Ⅰ and control group (P>0.05). In anti-ICAM-1 group B, the radially arranged microvessels were very unclear around the sponges contrast to that of control group. New microvessels were very scarce in the center of the sponges.The number of the microvessels growing in the CAM mesenchymes around the sponges in the 2 subgroups were less than that in control group (P<0.01), and there was significant difference between the 2 subgroups (P<0.05). It was suggested that ICAM-1 could induce angiogenesis and support the survival of microvessels, and ICAM-1 was involved in embryonic angiogenesis.     

Expression of NF-κB in Schwann cells and its effect on motor neuron apoptosis in spinal cord following sciatic nerves injury in rats

Objective: To explore the expression of nuclear factor-kappa B (NF-κB) in Schwann cells (SCs) and its effect on motor neuron apoptosis in spinal cord following sciatic nerves injury in adult rats. Methods: Thirty-six adult Sprague-Dawley (SD) rats were divided randomly into normal control group (n=6), and sciatic nerves crushing group (n=30). and the later was further equally randomized into 5 subgroups: 1. 3. 7. 11. and 21 d post-injury groups. The expression of NF-κB of normal and injured nerves were examined by immunohistochemistry staining, and the apoptosis of motor neurons in spinal cord of lumbar 4 to lumbar 6 (L4-L6) was investigated by terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling (TUNED assay. Both were quantitated by image analysis. Results: In crushing group, except 21 d post-injury group, the expression of NF-κB was markedly higher than that in the normal control group (P<0. 05,P<0. 01). At 1 d after sciatic nerves crushing, the expression of NF-κB was obviously up-regulated, reached peak at 3 d. and recovered at 21 d. The same trend was observed in the time-course on motor neuron apoptosis after sciatic nerves injury. Correlation analyses revealed that motor neuron apoptosis was significantly and positively correlated with the expression of NF-κB following sciatic nerves injury (r=0. 976 0,P<0. 01). Conclusion: After injury of sciatic nerves, the presence and up-regulation of NF-κB in SCs may be involved in motor neuron apoptosis in L4-L6 spinal cord.