蛇床子素对体外培养大鼠股骨组织吸收的抑制作用

目的:研究蛇床子素(Osthole,OS)对体外培养股骨组织(骨干和骨骺端)吸收活性的影响.方法:取(80±5)g雄性SD大鼠6只,体外分离培养大鼠股骨组织的骨干和骨骺端,随机分为对照组、雌二醇组(estradiol,E)和蛇床子素处理组.同时在股骨组织分离培养48 h后采用终浓度为1×10^-5 mol/L的蛇床子素和1×10^-8 mol/L雌二醇对体外培养骨干和骨骺端进行处理;分别在药物处理后的第3、6、9、12 天后测定抗酒石酸酸性磷酸酶(Tartrate-resistant acid phosphatase,StrACP)活性、培养基中葡萄糖(Glucose,Glu)、乳酸(Lactic acid,La)的含量、Real-Time RT-PCR StrACP、集落刺激因子(Macrophage colony stimulating factor,MCSF)、组织激酶K(Cathepsin K,CTSK)mRNA的表达水平.结果:1×10^-5 mol/L蛇床子素组碱性磷酸酶(ALP)活性为2226,1×10^-8 mol/L雌二醇组ALP活性为2498;与对照组比较1×10^-5 mol/L蛇床子素和1×10^-8 mol/L雌二醇组在加药处理后的第6、9和12天显着抑制StrACP酶活性(P<0.05);1×10^-5 mol/L蛇床子素处理体外培养大鼠骨骺端和骨干组织的第3、6、9天后显着增加培养基中乳酸含量(P<0.05),并显着减少培养基中葡萄糖的含量(P<0.05).与对照组比较,1×10^-5 mol/L的蛇床子素处理体外培养大鼠股骨组织后的第6和9天蛇床子素能显着抑制StrACP,MCSF,CTSK mRNA的表达水平(P<0.05).结论:蛇床子素抑制体外培养大鼠股骨组织的骨骺端和骨干吸收活性同时可提高营养代谢水平.     

蛛网膜下腔出血后脑出血痉挛的观察及护理

目的探讨尼莫地平在治疗蛛网膜下腔出血后脑血管痉挛的临床疗效及其护理。方法回顾性调查分析我院2007年7月至2011年2月37例蛛网膜下腔出血后脑血管痉挛患者的临床资料。结果 37例经尼莫地平治疗和精心护理,痊愈14例,好转12例,有严重后遗症5例,死亡6例,病死率16.2%。结论常规治疗基础上加用静脉滴注尼莫地平可显著降低迟发性VCS的发生,改善SAH的预后。     

脑梗死治疗研究现状与进展

脑梗死又称缺血性脑卒中,占全部卒中的70％～80％,是指各种原因所致脑部血液供应障碍,导致脑组织缺血、缺氧性坏死,出现相应的神经功能缺损症状。脑梗死包括脑血栓形成、脑栓塞、腔隙性脑梗死,属于中医学“中风”范畴。本文就脑梗死中医、西医及中西医结合治疗脑梗死研究现状及进展作一综述。     

盖诺与多西他赛联合铂类治疗晚期非小细胞肺癌的临床观察

肺癌是最常见的恶性肿瘤，肺癌中约75％～80％为非小细胞肺癌（NSCLC），在我地区高发，约70％患者发现时已属中晚期，失去手术时机。故我科2002年～2006年间分别用盖诺与国产多西他赛联合铂类方案治疗晚期小细胞肺癌约36例，现将其两组的近期临床疗效及毒副反应报告如下。     

精神科老年病房护理安全风险因素分析及对策

目的对影响精神科老年患者护理安全风险因素进行评估分析,制定相应护理对策,为老年患者提供安全优质的护理服务。方珐对精神科老年病房2007--2012年发生的护理安全问题进行回顾性分析。结果影响精神科老年住院患者安全的风险因素主要有：跌倒（摔倒）、坠床、误吸与窒息（噎食）、皮肤损伤（压疮、烫伤）。结论通过对精神科老年病房护理安全风险因素进行分析,提倡风险管理、预见性护理,采取有针对性的护理对策,减少护理纠纷发生,提高护理安全。     

精神科病房暴发感染性腹泻的护理

11例感染性腹泻患者,发生在精神科病房,在立即采取紧急治疗及护理的同时,予以严密消毒隔离,控制传染源,切断传播途径,保护易感人群。经积极处理,本组患者均获痊愈,病房解除隔离,恢复常态。     

Noth2调控舌鳞癌干细胞的增殖、侵袭和迁移

BACKGROUND:Previous studies have found that the expression of Notch2 is up-regulated in tongue squamous cell carcinoma (TSCC) stem cells, but the effect of Notch2 on TSCC remains unclear. OBJECTIVE:To explore the effect of Notch2 on the proliferation, invasion and migration of TSCC stem cells (ALDH^br). METHODS:ALDHbr cells were enriched and separated by fluorescence-activated cell sorting technique from Tca8113 cells cultured in using serum-free medium, and Notch2 expression in ordinary TSCC cells (ALDH^low) and ALDH^br cells was detected by western blot. The ALDH^br cells transfected with Notch2 shRNA (sh-Notch2) by liposome served as experimental group, and ALDH^br cells transfected with sh-control were as control group. The effects of Notch2 on the proliferation, invasion and migration of ALDH^br cells were detected by cell counting kit-8 kit and Transwell assay, respectively. In addition, the expression levels of Ki67, matrix metalloproteinases 2 and 9 protein in Notch2-silenced ALDH^br cells and control cells were detected by western blot. RESULTS AND CONCLUSION:The expression level of Notch2 in ALDH^br cells was significantly higher than that in ALDH^low cells (P < 0.05). The ALDHbr cell proliferation ability and Ki67 protein expression in the experimental group were obviously lower than those in the control group (P < 0.05). The ALDH^br cell invasion and migration abilities in the experimental group were significantly lower than those in the control group (P < 0.05). The expression levels of matrix metalloproteinases 2 and 9 protein in the experimental group were obviously lower than those in the control group (P < 0.05). All these results show that Notch2 can promote the proliferation, invasion and migration of ALDH^br cells, which suggests that Notch2 plays an important role in the occurrence and development of TSCC, and it may be a potential molecular target for the treatment of TSCC.     

奥沙利铂联合氟尿嘧啶、亚叶酸钙治疗晚期大肠癌疗效观察

大肠癌是我国六种常见癌症之一，死因居恶性肿瘤第4～6位．发病率逐年上升。作者自2000年4月至2003年8月间应用奥沙利铂、氟尿嘧啶及亚叶酸钙治疗晚期大肠癌18例。现将结果报告如下。     

灾后心理康复之北川模式

北川是5.12汶川地震极重灾区,灾后心理问题呈现复杂、多元等特点,为优化整合各种心理救助资源,北川县人民政府于2009年4月25日成立了全国首个心理卫生服务县直机构——北川心理卫生服务中心,负责整合全县心理救助资源,组     

加味失笑散治疗冠心病心绞痛的临床应用及作用机理研究进展

失笑散属理血剂,具活血化瘀作用,它由五灵脂和蒲黄两味药组成。用于心腹诸痛或少腹急痛以及妇女月经不调或产后恶露不行。前人运用本方,患者每于不觉中,诸症悉除,不禁欣然而笑,