大型中医药知识图谱构建研究

知识图谱(Knowledge Graph)是以"语义网络"为骨架构建起来的巨型、网络化的知识系统,能捕捉并呈现领域概念之间的语义关系,使各种信息系统中琐碎、零散的知识相互连接,支持综合性知识检索以及问答、决策支持等智能应用。探索如何构建面向中医药领域的知识图谱,实现中医药知识资源的有效整合,面向中医药工作者和百姓提供全面、及时、可靠的知识服务。     

嵌入式临床智能决策支持系统设计与中医临床知识服务研究

随着医院信息化水平的不断提高,在医院信息系统中开展中医药知识服务不仅具有可行性,且对中医药临床科研的推动极具潜力。研究通过构建嵌入式临床智能决策支持系统与中医临床知识服务平台的开发,提供嵌入电子病历的实时临床决策支持和与临床病例高度相似的典型案例匹配,以及基于临床临床情景的诊疗规范、名家经验、名医名方、临床证据等中医临床知识服务。     

中医名医医案分析系统研究

目的:通过建立"中医名医医案分析系统",密切结合临床需求,通过对名医临床病案的信息抽取和数据挖掘,为临床医生提供针对性的知识服务。方法:"中医名医医案分析系统"分为名医信息管理模块、临床医案导入模块、数据标准化模块、医案数据管理模块、医案数据分析模块和数据字典管理模块六个模块。结果:该系统可通过导入名医经验案例,对其进行管理、规范化处理和数据分析。结论:"中医名医医案分析系统"能够辅助中医名医经验研究和学习传承,但在术语研究和指导临床实践方面尚存在一定的问题。需要继续探索符合中医临床特点的中医临床病案术语系统,以支持名医经验研究。     

中医药知识资源浅析

目的：对中医药领域中的知识资源进行分类辨析,为中医药知识管理与知识服务研究提供参考。方法：根据知识管理理论,通过文献调研,对中医药领域知识资源进行分类,并对每一类知识资源进行分析和说明。结果：中医药领域的知识资源除了传统的专家隐性知识和文献之外,还包括多媒体档案、数据库、知识库和本体等多种新型的知识资源。结论：随着中医药信息化的发展,涌现出了中医药领域中出现的新型知识资源,将引发中医药知识管理模式与方法的创新。     

面向中药数据库的语义集成框架

中药数据库具有数量众多、异构性强、高度分散、自治性高等特点,如何实现中药数据库的集成访问,消除中药领域的"信息孤岛"现象,仍是中药信息学领域悬而未决的重要问题。研究旨在基于语义网技术,实现专门面向中药数据库的语义集成框架。构建一个简单的中药领域本体,建立本体与中药数据库之间的映射关系,实现针对多个中药数据库的统一查询机制,从而为各种中药数据应用提供一个统一的平台。     

围绝经期综合征的中医药治疗进展

围绝经期综合征(perimenopause syndome)又称更年期综合征(climacteric syndome),是由于卵巢功能退行性改变。脑垂体功能亢进、雌激素水平低下所导致的植物神经功能紊乱为主,伴有心理症状的一组证侯群[1]。现就近年来中医药治疗围绝经期综合征的研究进展综述如下。     

方剂相似度探析

利用现代信息方法计算方剂之间的相似程度,以中药为基本单元,从中药组成、中药组成剂量、中药功效3个层次进行方剂之间的相似程度计算,其中中药组成相似度是利用Jaccard集合相似度比较法计算,中药组成剂量的相似程度及方剂功效的相似度利用夹角余弦比较法计算。从计算方法角度探索方剂之间的相似程度。     

Expressions and significances of Nek2 and β-catenin in breast invasive ductal carcinoma

Objective To investigate the expression and significance of NIMA related kinases 2 ( Nek2) and β-catenin (β-cat) in breast invasive ductal carcinoma (IDC) and concomitant ductal carcinoma in situ (DCIS). The roles of Nek2 and β-acat in the development and progression of breast cancer were explored. Methods The protein expression of Nek2 and β-cat in the tissues from 186 patients with IDC, 75 concomitant DCIS, 80 normal tissue adjacent to carcinoma and 40 fibroadenoma of breast was detected by using immunohistochemistry. Their relationship between clinicopathologic parameters was analyzed. Results There were correlations between the Nek2 expression in cytoplasm and grade(x2=8. 756;P<0.05) as well as tumor size (x2=6. 518;P<0.05) in IDC. The positive expression of Nek2 in nuclei was 32/186 and 15/75 in IDC and DCIS, respectively. There were correlations between the β-cat expression on the cytomembrane and grade (x2=45.493;P<0. 01) , TNM stage (x2=9. 510;P<0. 01), node status (Z=-2.035;P<0. 05) and estrogen receptor (ER) status (Z=-3. 004;P<0. 01). The β-cat expression on the cytoplasm was related with TNM stage (x2=8. 194;P<0. 05). The expression of Nek2 and β-cat had no correlation with clinicopathologic parameters in concomitant DCIS (P>0. 05), the Nek2 expression in cytoplasm had a correlation with the β-cat expression in cytoplasm (r=0. 226,P<0.05) in concomitant DCIS, and the same relationship could also be seen in IDC (r=0. 368,P<0.01). There was significant difference in the β-cat expression on the cytomembrane between IDC and concomitant DCIS (Z=-3. 804,P<0. 01). In the normal tissue adjacent to carcinoma and fibroadenoma, the Nek2 expression in cytoplasm and nuclei Was low or negative;the β-cat expression on the cytomembrane was high but that Was low in cytoplasm.Conclusion Centrosome regulatory factor Nek2 and β-cat can support a new way to explore the mechanisms of breast tumorigrenesis.And they may become a new antitumor drug target in the future.     

姜黄素对抑郁症大鼠模型行为学及神经肽Y的影响

目的 姜黄素的抗抑郁样作用及对慢性应激抑郁大鼠模型海马、下丘脑、血浆中神经肽Y(NPY)含量的影响.方法 SD雄性大鼠24只,用随机区组设计的方法分为对照组、模型组、盐酸氟西汀组、姜黄素组,处理21 d后,观察1%蔗糖水摄取率和强迫游泳不动时间,用竞争酶联免疫分析法测定NPY含量.结果 在处理21 d后,姜黄素组、氟西汀组大鼠与模型组大鼠比较,强迫游泳不动时间明显缩短(P<0.05),1%蔗糖水摄取量明显升高(P<0.01)达到对照组水平;在处理21 d后,正常组、姜黄素组、氟西汀组大鼠与模型组大鼠比较,海马、下丘脑NPY含量变化无统计学差异;在处理21 d后,对照组大鼠血浆NPY含量与模型组有显著差异(P<0.01),氟西汀组、姜黄素组、模型组大鼠血浆NPY含量无统计学差异.结论 姜黄素具有抗抑郁样作用,其效果与一线抗抑郁药盐酸氟西汀相仿. 本次结果尚不足以证明姜黄素可以通过调节NPY含量发挥抗抑郁作用,其抗抑郁样作用尚需进一步研究.     

雄激素受体在乳腺癌细胞中的表达及其对细胞增殖的影响

Objective To evaluate the expression of androgen receptor (AR) in the breast cancer cell lines and its effect on proliferation of breast cancer cells. Methods The estrogen receptor (ER) -positive MCF-7 and ER-negative MDA-MB-453 cells were involved in this study and cultured in vitro. The expression of AR was detected by using Western blotting. Cell proliferation was determined by methyl thiazol tetrazolium (MTT) assay after the treatment with different concentrations of dihydrotestosterone (DHT) ( 1 x 10 -7, 1 x 10- 8, 1 x 10 -9 mol/L) for 48, 96 and 144 h respectively. Cell cycle was analyzed by flow cytometry following culture for 72 h. Results DHT increased the AR expression in the two breast cancer cell lines. AR pathway could inhibit proliferation of MCF-7 and MDA-MB-453 cells. There was no significant difference in absorbance values among three treatment groups at different time points (P ＞ 0. 05). Cell cycle analysis revealed that the proportion of cells at G1 phase was increased, and that at S phase decreased. Conclusion AR pathway may inhibit proliferation of ER-negative MDA-MB-453 breast cells as well as ER-positive MCF-7 cells, by suppressing the process of G1 to S phase progression.