医绩卓著 誉满陇上 承前启后 丰功永存——张汉祥生平及学术思想介绍

张汉祥(1911～1983年),天津市人,甘肃当代著名中医。他毕生致力于继承和发展祖国的医学事业,在近半个世纪的医疗实践中,医绩卓著,誉满陇原,深受甘肃人民的信赖和崇敬。1935年张老拜北平名医方伯屏先生为师,学习中医,他以强烈的求知欲望,至诚的学习决心,勤求古训,博采众方,耽嗜典籍,如啖蔗饴,终于达到通篇背诵《伤寒     

化石汤对泌尿系结石影响的扫描电镜观察

中医治疗泌尿系结石已积累了丰富的经验。七十年代时我国开展中西医结合疗法治疗本征,排石率达68％。有报导用中西医结合疗法治疗泌尿系结石,可碎石和溶石,但上述报道,多都根据肉眼及X线照片观察,用扫描电镜(SEM)观察,则尚未见报导。现将我们对3例结石的观察结果,报告于后,并作简要的讨论。材料与方法我院泌尿结石中医专科门诊,五年来共治疗病人152人。排石率为72.5％。本材料选自经治愈的典型病例3例,观察了此3例患者服药后排出之结石标本共4粒。按扫描电镜标本制备法,将标本水洗后,自然干燥,用     

体外诱导骨髓间充质干细胞向肾小管上皮样细胞分化

Objective To investigate the differentiation of rat bone marrow mesenchymal stem cells (MSCs) to renal tubular epithelial-like cells under different conditions. Methods MSCs were obtained from rat marrow. MSCs were isolated by gradient density centrifugation and plastic adherence and then purified. Surface markers were identified with flow cytometry after amplification in vitro. The purified MSCs of the third passage were cultured respectively as follows: (1) control group: DMEM medium with fetal bovine serum(FBS). (2) all-trans retinoic acid (ATRA) group: DMEM medium with FBS, ATRA and ischemic reperfusion-injured kidney tissue homogenate. (3)combination group: DMEM medium with FBS, ATRA, ischemic reperfusion-injured kidney tissue homogenate, epidermal growth factor (EGF) and bone morphogenetic protein 7 (BMP-7). After 7 days, the MSCs were collected for alkaline phosphatase (AKP) staining, cytokeratin-18 and E-cadherin immunocytochemical analysis. Results The positive rates of the third passage MSCs in CD44, CD90 and CD29 were 97.8%±0.9%, 96.8%±1.4% and 97.6%±2.4%,respectively, but in CD11b/c and CD34 were only 13.2%±0.6% and 1.2%±0.5%. The MSCs in control group were spindle. The MSCs in ATRA group were round and elliptic. The MSCs in combination group became cobblestone-like cells after 7 days. AKP staining showed that tubular epithelial-like cells from MSCs in control group were negative, some above cells in ATRA group were positive and number of above cells increased in combination group. Compared with negative control group, the ratios of cytokeratin-18 positive cells in ATRA group and combination group were respectively increassed by 29.47%±1.08% and 47.52%±2.13% (all P<0.05), the ratios of E-cadherin positive cells in ATRA group and combination group were respectively increased by 14.88%±2.46% and 36.15%±1.13% (all P<0.05). Conclusion MSCs may differentiate by renal tubular epithelial-like cells under the induction of ischemic reperfusion-injured kidney tissue homogenate and ATRA in vitro, which are further differentiated under the combined induction of EGF and BMP-7.     

环丙沙星外用治疗新生儿脓疱疹的护理

新生儿脓疱疹是一种新生儿期常见的化脓性皮肤病,临床上常规采用静脉给予抗生素治疗,病程长,用药量大,副反应大,效果欠佳.本组将环丙沙星注射液直接涂抹于新生儿疱疹周围,使药液直接作用于破损的黏膜上,达到治愈新生儿脓疱疹的目的.     

社区护士的护理活动现状分析

目的 通过对社区护理活动的分析，为社区护理的规范化管理提供基础资料。方法 采用直接观察和内容分析法，观察研究延边第二人民医院社区卫生服务中心的管理体系及工作运行，包括行政管理、人员结构、服务形式及服务内容，分析了社区护理服务工作记录。结果 机构的负责人是护士，医生和护士之比为1：3。服务形式为建立家庭病床或保健合同方式，从医院追随着社区进行连续性管理的同时，通过健康调查发现患者并主动提供服务，培训下岗人员后提供给需要护理员的家庭，老年人群的健康教育等，护理活动包括基础护理、保健指导、诊疗个部分，护理活动性质多属于二级预防，从本机构的人群疾病特性看，脑梗死最多，其次是糖尿病。结论 应扩大护理的活动范围，加强护士与医生及其他部门的合作，强化护士的安全意识，开发适合社区需要的护理服务项目等。