全文获取类型
收费全文 | 792篇 |
免费 | 43篇 |
国内免费 | 9篇 |
专业分类
耳鼻咽喉 | 13篇 |
儿科学 | 10篇 |
妇产科学 | 18篇 |
基础医学 | 106篇 |
口腔科学 | 13篇 |
临床医学 | 84篇 |
内科学 | 170篇 |
皮肤病学 | 8篇 |
神经病学 | 107篇 |
特种医学 | 14篇 |
外科学 | 80篇 |
综合类 | 1篇 |
一般理论 | 1篇 |
预防医学 | 49篇 |
眼科学 | 10篇 |
药学 | 113篇 |
中国医学 | 2篇 |
肿瘤学 | 45篇 |
出版年
2023年 | 6篇 |
2022年 | 18篇 |
2021年 | 41篇 |
2020年 | 19篇 |
2019年 | 29篇 |
2018年 | 28篇 |
2017年 | 14篇 |
2016年 | 19篇 |
2015年 | 19篇 |
2014年 | 40篇 |
2013年 | 36篇 |
2012年 | 55篇 |
2011年 | 52篇 |
2010年 | 34篇 |
2009年 | 29篇 |
2008年 | 52篇 |
2007年 | 39篇 |
2006年 | 48篇 |
2005年 | 36篇 |
2004年 | 56篇 |
2003年 | 27篇 |
2002年 | 35篇 |
2001年 | 4篇 |
2000年 | 6篇 |
1999年 | 8篇 |
1998年 | 3篇 |
1996年 | 3篇 |
1993年 | 2篇 |
1992年 | 5篇 |
1990年 | 4篇 |
1989年 | 6篇 |
1988年 | 6篇 |
1987年 | 6篇 |
1986年 | 2篇 |
1984年 | 5篇 |
1983年 | 6篇 |
1982年 | 2篇 |
1979年 | 4篇 |
1977年 | 8篇 |
1976年 | 1篇 |
1975年 | 5篇 |
1974年 | 2篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1971年 | 4篇 |
1970年 | 2篇 |
1969年 | 2篇 |
1968年 | 3篇 |
1967年 | 3篇 |
1966年 | 1篇 |
排序方式: 共有844条查询结果,搜索用时 31 毫秒
41.
Babenko AI 《Problemy sot?sial?no? gigieny, zdravookhranenii?a i istorii medit?siny / NII sot?sial?no? gigieny, ?konomiki i upravlenii?a zdravookhraneniem im. N.A. Semashko RAMN ; AO "Assot?siat?sii?a 'Medit?sinskai?a literatura'."》2002,(2):8-13
Time course of population mortality in Siberia in 1985-1999, mean life span of men and women, major mortality causes in the neonatal and capable age are presented. Population health loss resultant from mortality, morbidity, and disability is evaluated. 相似文献
42.
Christine L Powell Oksana Kosyk Pamela K Ross Robert Schoonhoven Gunnar Boysen James A Swenberg Alexandra N Heinloth Gary A Boorman Michael L Cunningham Richard S Paules Ivan Rusyn 《Toxicological sciences》2006,93(1):213-222
Toxicogenomics provides the ability to examine in greater detail the underlying molecular events that precede and accompany toxicity, thus allowing prediction of adverse events at much earlier times compared to classical toxicological end points. Acetaminophen (APAP) is a pharmaceutical that has similar metabolic and toxic responses in rodents and humans. Recent gene expression profiling studies with APAP found an oxidative stress signature at a subtoxic dose that we hypothesized can be phenotypically anchored to conventional biomarkers of oxidative stress. Liver tissue was obtained from experimental animals used to generate microarray data, where male rats were given APAP at subtoxic (150 mg/kg) or overtly toxic (1500 and 2000 mg/kg) doses and sacrificed at 6, 24, or 48 h. Oxidative stress in liver was evaluated by a diverse panel of markers that included assessing expression of base excision repair (BER) genes, quantifying oxidative lesions in genomic DNA, and evaluating protein and lipid oxidation. A subtoxic dose of APAP produced significant accumulation of nitrotyrosine protein adducts. Both subtoxic and toxic doses caused a significant increase in 8-hydroxy-deoxyguanosine (8-OH-dG) as well as a significant decrease in glutathione (GSH) content. Only toxic doses of APAP significantly induced expression levels of BER genes. None of the doses examined resulted in a significant increase in the number of abasic sites or in the amount of lipid peroxidation. The accumulation of nitrotyrosine and 8-OH-dG adducts along with reduced GSH content in the liver phenotypically anchors the oxidative stress gene expression signature observed with a subtoxic dose of APAP, lending support to the validity of gene expression studies as a sensitive and biologically meaningful end point in toxicology. 相似文献
43.
Vladimir N Babenko Malay K Basu Fyodor A Kondrashov Igor B Rogozin Eugene V Koonin 《BMC cancer》2006,6(1):36
Background
Carcinogenesis typically involves multiple somatic mutations in caretaker (DNA repair) and gatekeeper (tumor suppressors and oncogenes) genes. Analysis of mutation spectra of the tumor suppressor that is most commonly mutated in human cancers, p53, unexpectedly suggested that somatic evolution of the p53 gene during tumorigenesis is dominated by positive selection for gain of function. This conclusion is supported by accumulating experimental evidence of evolution of new functions of p53 in tumors. These findings prompted a genome-wide analysis of possible positive selection during tumor evolution. 相似文献44.
Sarah Furtado Haydeh Payami Paul J Lockhart Melissa Hanson John G Nutt Andrew A Singleton Amanda Singleton Jamel Bower Ryan J Utti Thomas D Bird Raul de la Fuente-Fernandez Yoshio Tsuboi Mary L Klimek Oksana Suchowersky John Hardy Donald B Calne Zbigniew K Wszolek Matthew Farrer Katrina Gwinn-Hardy A Jon Stoessl 《Movement disorders》2004,19(6):622-629
Spinocerebellar ataxia type 2 (SCA2) has been recognized recently as an uncommon cause of parkinsonism, an alternate presentation to the typical cerebellar disorder. This research review summarizes the existing literature on parkinsonism-predominant presentation SCA2 and presents new clinical cases of patients with this condition. Various phenotypes are noted in this subtype of SCA2, including parkinsonism indistinguishable from idiopathic Parkinson's disease (PD), parkinsonism plus ataxia, motor neuron disease, and postural tremor. In several kindreds with multiple affected family members, the SCA2 expansion segregated with disease; in addition, several single cases of parkinsonism with and without a family history are also described. The number of repeats in symptomatic patients ranged from 33 to 43. Interruption of the CAG repeat with CAA, CGG, or CCG was found in some individuals, possibly stabilizing the repeat structure and accounting for the relative stability of the repeat size across generations in some families; allele length is not necessarily indicative of trinucleotide repeat architecture. Positron emission tomography scanning in one family showed reduced fluorodopa uptake and normal to increased raclopride binding with a rostrocaudal gradient similar to that found in idiopathic PD. This review emphasizes the importance of testing for SCA2 in patients with parkinsonism and a family history of neurodegenerative disorders. Testing for SCA2 is also important in studies of inherited parkinsonism. 相似文献
45.
46.
47.
48.
Bryan J Crane A Vila-Carriles WH Babenko AP Aguilar-Bryan L 《Current pharmaceutical design》2005,11(21):2699-2716
ATP-sensitive K+ channels, termed K(ATP) channels, provide a link between cellular metabolism and membrane electrical activity in a variety of tissues. Channel isoforms have been identified and are targets for compounds that both stimulate and inhibit their activity resulting in membrane hyperpolarization and depolarization, respectively. Examples include relaxation of vascular smooth muscle and stimulation of insulin secretion. This article reviews the cloning, molecular biology, and structure of K(ATP) channels, with particular focus on the SUR1/K(IR)6.2 neuroendocrine channels that are important for the regulation of insulin secretion. We integrate the extensive pharmacologic structure-activity-relationship data on these channels, which defines a bipartite drug binding pocket in the SUR (sulfonylurea receptor), with recent structure-function studies that identify domains of SUR and K(IR)6.2, the channel pore, which are critical for channel assembly, for gating, and for the ligand-receptor interactions that modulate channel activity. The atomic structure of a sulfonylurea in a protein pocket is used to develop insight into the recognition of these compounds. A homology model of K(ATP) channels, based on VC-MsbA, another member of the ABC protein family, is described and used to position amino acids important for the action of channel openers and blockers within the core of SUR. The model has a central chamber which could serve as a multifaceted binding pocket. 相似文献
49.
Yakar S Kim H Zhao H Toyoshima Y Pennisi P Gavrilova O Leroith D 《Pediatric nephrology (Berlin, Germany)》2005,20(3):251-254
We have created a liver-specific igf1 gene-deletion mouse model (LID) with markedly reduced circulating IGF-I levels. They demonstrate that while they have normal growth and development they develop insulin resistance secondary to the elevation of circulating growth hormone. When mated with an acid-labile subunit (ALS) gene-deleted mouse they also show osteopenia suggesting that circulating IGF-I levels play a significant role in bone formation. In a separate transgenic mouse we created a model of severe insulin resistance and type 2 diabetes by the overexpression of a dominant-negative IGF-I receptor in skeletal muscle. In this model we show that lipotoxicity plays a major role in the progression of the disease and is affected by treatment with a fibrate, which reverses the insulin resistance and diabetic state. These models are therefore very useful in studying human physiology and disease states.This work was presented in part at the IPNA Seventh Symposium on Growth and Development in Children with Chronic Kidney Disease: The Molecular Basis of Skeletal Growth, 1–3 April 2004, Heidelberg, Germany 相似文献
50.
Statins ameliorate endothelial barrier permeability changes in the cerebral tissue of streptozotocin-induced diabetic rats 总被引:6,自引:0,他引:6
Statins may have favorable effects on endothelial barrier function. The effect of rosuvastatin and simvastatin therapy (10 mg/kg) for 5 weeks on blood-brain barrier (BBB), blood-retinal barrier (BRB), and cardiac muscle permeability of streptozotocin-induced diabetic rats was studied. The size-selective permeability of different vascular beds to a group of fluorescein isothiocyanate dextrans of varying molecular weights was measured. The volume of distribution of 250-, 70-, and 40-kDa dextrans in the cerebral tissue of diabetic rats were significantly increased. The volume of distribution of these dextrans in cerebral tissue was normalized by both statins. Diabetes did not significantly alter the BRB, but both statins decreased the volume of distribution of 70- and 40-kDa dextrans in the retina. The volume of distribution of 40 kDa in cardiac muscle was increased in diabetes, and this change was prevented with statin treatment. Treatment with rosuvastatin and mevalonate (150 mg/kg in drinking water for 5 weeks) did not alter the volume of distribution measurements. We concluded that 1) diabetes in rats is associated with significant changes in the BBB permeability; 2) statin treatment improves the endothelial barrier function in cerebral tissue, retina, and cardiac muscle; and 3) this statin effect could not be attributed to HMGCoA reductase inhibition. 相似文献