腹腔镜子宫次全切除术中超声刀的应用

目的　探讨超声刀在腹腔镜子宫次全切除术中的应用价值。方法　 2 0 0 2年 1～ 12月在腹腔镜下分别应用超声刀和双极电凝及剪刀进行子宫次全切除术 ,比较术中出血量、手术时间、术后平均住院日及肛门排气时间。结果　超声刀组的平均手术时间、术中平均出血量显著少于双极电凝组 (P <0 0 1) ,术后平均住院日及肛门排气时间在两组间差异无显著性 (P >0 0 5 )。结论　超声刀可应用于妇科腹腔镜子宫次全切除术 ,由于其操作简单、安全可靠 ,值得推广。     

针刺对吗啡戒断大鼠脑组织一氧化氮合酶基因表达的影响

目的 :观察针刺对吗啡戒断大鼠脑组织神经元一氧化氮合酶基因 (nNOSmRNA)表达的影响 ,探讨针刺改善戒断症状的分子生物学机理。方法 :建立大鼠吗啡自然戒断模型 ,运用逆转录聚合酶链反应 (RT PCR)测定戒断大鼠脑组织nNOSmRNA的表达。观察戒断后 ,针刺“足三里”和一氧化氮合酶 (NOS)抑制剂L N 硝基精氨酸 (L NAME)对吗啡戒断大鼠戒断症状和脑组织nNOSmRNA表达的影响。结果 :戒断组nNOSmRNA表达增加 ,针刺组和L NAME组nNOSmRNA表达比戒断组减少。针刺组戒断积分比戒断组少 ,组间差异显著 (P <0 .0 1 )。结论 :针刺“足三里”穴具有抑制吗啡戒断大鼠脑组织nNOSmRNA表达的作用 ,提示这可能是针刺改善吗啡戒断症状的一个重要机制。     

Overexpressed eIF4E is functionally active in surgical margins of head and neck cancer patients via activation of the Akt/mammalian target of rapamycin pathway.

PURPOSE: Overexpression of eIF4E in surgical margins of head and neck cancer patients is an independent risk factor for recurrence. We hypothesize that overexpressed eIF4E is functionally active in tumor margins through activation of the Akt/mammalian target of rapamycin (mTOR) pathway EXPERIMENTAL DESIGN: Western blots and/or immunohistochemistry were performed to determine whether phosphorylation of mTOR and activation of its downstream molecules eIF4E-binding protein-1 (4E-BP1) and p70 S6 kinase and the upstream modulator of mTOR, Akt, were expressed in margins overexpressing eIF4E. RESULTS: There was a significant association between phospho-4E-BP1 and eIF4E expression of a margin or a significant difference in phospho-4E-BP1 expression between the eIF4E-positive and -negative margins (P < 0.01). A significant association between eIF4E and phospho-p70 S6 kinase as well as eIF4E and phospho-mTOR was also noted (P < 0.05). Western blot analysis indicated a highly significant difference in the phosphorylation status of 4E-BP1 between tumors and resection margins. A total of 89% of the 4E-BP1-expressing margins expressed more of the phosphorylated (beta, gamma, and delta) isoforms, whereas 81% of the 4E-BP1-expressing tumors expressed more of the unphosphorylated alpha isoform. A similar difference in Akt activation was noted between eIF4E-positive margins and tumors (P < 0.05). CONCLUSIONS: Overexpression of eIF4E is functionally active in tumor margins through activation of the Akt/mTOR signaling pathway. The greater degree of expression of downstream targets and upstream regulators of mTOR in margins compared with the tumors indicates preferential activation of the Akt/mTOR signaling pathway in margins overexpressing eIF4E. Rapamycin analogs can potentially be used as adjuvant therapy for patients with eIF4E-positive margins.     

加味通关液超声雾化吸入治疗Ⅱ型呼吸衰竭的临床观察

为观察中药超声雾化对呼吸衰竭的疗效,采用本院研制的加味通关液超声雾化吸入治疗Ⅱ型呼吸衰竭患者27例,并与可拉明组30例对照。结果显示:治疗组临床治愈显效率70.3％,SaO_2上升达38.4％,咳嗽、呼吸困难、咯痰好转率、平均治疗时间与对照组比较均有显著性差异(P<0.05)。     

急性上消化道出血112例临床疗效分析

采用国家中医药管理局颁布的《中医血证治疗常规》治疗急性上消化道出血112例，取得了较好疗效，总有效率达94．6％。进行了证和疗效，原发病和疗效，病情程度和疗关系的分析。说明中医血证治疗效常规在临床实践中是切实可行的。     

P型ATP酶阻滞剂和激动剂对肝豆状核变性成纤维细胞胞浆铜的影响

探讨P型ATP酶阻滞剂、激动剂对肝豆状核变性患儿离体培养皮肤成纤维细胞胞浆铜含量的影响。方法采用高浓度铜及P型AFP酶阻滞剂（矾酸钠）、激动剂（长春新碱）孵育细胞，分析孵育6、12、24小时后，肝豆状核变性患儿、杂合子及正常儿童成纤维细胞胞浆中铜含量的变化。结果铜及长春新碱孵育后，患儿成纤维细胞胞浆钢水平在各时点均高于杂合子及正常儿童。铜及矾酸钠孵育后，患儿、杂合子及正常儿童胞浆铜含量在各时点均明显少于其他条件孵育后。结论患儿钢转运P型ATP酶对P型ATP酶阻滞剂的敏感性与杂合子及正常儿童相同，对激动剂的敏感性异常。P型ATP酶能减少患儿成纤维细胞对铜的摄取，在肝豆状核变性的治疗方面有一定的提示意义。