低位直肠癌保留肛门的综合治疗

目的探讨低位直肠癌保留肛门对其预后的影响。方法对25例低位直肠癌患者行术前放疗、化疗。术式：行全直肠系膜切除术（total mesorectal excision，TME），直肠分离至肛提肌水平。手术远端切缘超过肿瘤下缘1．0-2．0cm。均保留肛门。术后再给予放疗和化疗。结果全组无手术死亡，无吻合口瘘和吻合口狭窄发生。大部分患者术后3～6个月大便控制良好。随访1～37个月，仅有1例复发，1例死于心脏病。结论对于低位直肠癌患者，配合术前术后放、化疗，采用TME手术，不仅可以保留患者肛门，避免了人造肛门所致的心理影响，而且同样可以取得较好疗效。     

试论七情发生和脑主神明与抑郁症病机证治的关系

抑郁症是一组以抑郁心境自我体验为中心的临床症状群或状态.中医多将其归在郁证之类,对其理论解释的重点多是在心主神明论指导下的以五脏为中心的肝,临床治疗亦多遵疏肝解郁这一基本大法,并取得了一定成效.     

大肠侧向发育型肿瘤细胞株双向电泳技术成功建立

目的:建立侧向发育型肿瘤细胞(LST)株双向电泳技术.方法:提取LST细胞株总蛋白质,采用不同pH范围IPG胶条进行LST细胞株总蛋白质双向电泳,并对实验步骤进行一系列的调整优化.结果:获得了较为清晰的LST细胞株双向电泳图谱,采用pH 3～10线性IPG胶条分离出1356±43个蛋白质斑点;pH 4～7 IPG胶条两种上样量(250μg和150μg)分别分离出了1285±51和989个蛋白质斑点.结论:通过相关条件的调整优化,成功建立了双向电泳技术,为研究LST特殊性生物学行为相关蛋白质奠定了坚实的基础.     

人工合成铁（Ⅱ）半胱氨酸SOD模拟物的毒性及血清半衰期研究

本文对人工合成的铁（Ⅱ）半胱氨酸SOD模拟物的毒性及其吸收后血清半衰期进行了初步探索。通过灌胃急性毒性、慢性毒性、遗传毒性和灌胃后吸收入血清的药时变化进行了研究。研究结果表明：此物对小鼠灌胃的LD50为大干5000mg／kg。连续给药1月后，小鼠的心、肝、胸腺、脾、肺、肾和肠胃等脏器在形状、大小和颜色上与正常组织无明显差异；用800mg／kg的剂量给小鼠灌胃7d，小鼠的晴多染红细胞微核率有一定增长（P〈005）；经不同浓度给药后，血清中血药浓度峰值时间为60-210min，半衰期为90-180min，证明此药为微毒物质，适当剂量口服后无明显蓄积毒性，大剂量使用时可导致一定的染色体损伤，血清药浓度半衰期随给药浓度有所变化。此药可望在植物生长调节剂、医药或化妆品、饲料、食品中的抗氧化添加剂及着色抑菌的防腐添加剂等中代替天然SOD。     

Studies of the human oropharyngeal airspaces using magnetic resonance imaging IV--the oropharyngeal retention effect for four inhalation delivery systems.

Magnetic resonance imaging (MRI) of the oropharyngeal region from 20 adult volunteers using four model inhalation devices (varying mouthpiece diameters, airflow resistances) and tidal breathing was carried out. Statistical analysis (convex hull method) selected 12 scans from 80 data sets representing the extremes of all dimensions in the population. Twelve physical mouth-throat models were made by stereolithography using the exact scan data. The aim was to produce models with varying dimensions to span the adult population, and to investigate if oropharyngeal dimensions affected throat retention for different delivery systems. In an in vitro analysis, the models were used to determine the retention effect of the oropharyngeal airspaces when drug aerosols were administered from four inhalation delivery systems: a pressurised metered dose inhaler (pMDI), two different dry powder inhalers (DPIs A and B), and a nebulizer. The aims of this work were to determine the key parameters governing mouth-throat retention and whether retention was dependent on the delivery system used. Characterizing the throat models by measuring 51 different dimensional variables enabled determination of the most influential variables for dose retention for each inhalation delivery system. Throat model retention was found to be dependent on the delivery system (pMDI approximately DPI(A) > DPI(B) > Neb.). The most influential variable was the total throat model volume. Throat models representing high, median, and low oropharyngeal filtration in healthy adults have been identified.     

自体表皮细胞培养与异体真皮组合应用研究

严重烧伤病人皮肤修复中主要未解决的问题是真皮的替代。动物实验结果表明，异体皮移植后５天，用自体培养表皮细胞膜片覆盖真皮床，１４天后复合皮成活率是８４．６％±２．４％。组织学检查证实表皮已形成了复层结构，可见基底层、颗粒层和角质层。临床应用中，异体皮移植后１０天，去除异体表皮覆盖病人的自体培养表皮，３５天后未见排斥征象，异体真皮促进了培养表皮的分层、成熟和完整，组织学检查证实表皮细胞的边缘清楚，已分化形成颗粒层和角质层，真皮多细胞，已血管化，但表皮嵴缺乏。     

对一宗入境二级放射货包的监测结果分析

汕头卫生检疫局空港处对汕头航空口岸—宗入境二级货包的监测过程及对有关运输工作人员作了放射知识问卷调查。从中发现。运输单位货物存放设施落后。有关工作人员放射卫生知识欠缺，对放射污染可能造成的危害缺乏正确的认识。为此，笔者提出了自己的看法，以期对放射监测工作的进一步完善提供参考。     

β—半乳糖苷酶核酸免疫初探

将带有编码β－半乳糖苷酶基因片段的质粒ｐＳＶ－β－Ｇａｌａｃｔｏｓｉｄａｓｅ通过股四头肌接种小鼠，利用ＥＬＩＳＡ方法检测鼠体内β－半乳糖苷酶获得得表达后刺激机体产生抗β－半乳糖苷酶抗体的情况。     

Intraportal allogeneic transplantation of the islets of Langerhans cultures in the complex treatment of surgical patients with diabetes mellitus

The article presents results of treatment of 21 patients with a surgical pathology and diabetes mellitus who had intraportal allotransplantation of cultures of islet cells of the pancreas and 316 patients with the similar surgical pathology without such transplantation. It was found that the intraportal introduction of islet cell cultures into the liver can result in an early and considerable drop of the injected insulin dose which is very important in the postoperative period. This method allowed to make the period of treatment at the hospital approximately 15 days shorter.