CircCDR1as Suppresses Bone Microvascular Endothelial Cell Activity and Angiogenesis Through Targeting miR‐135b/ FIH‐1 Axis

ObjectiveThe current study investigated the role of CircCDR1as on angiogenesis of bone microvascular endothelial cells (BMECs) isolated from non‐traumatic ONFH.MethodsForty corticosteroid‐induced ONFH patients received THA were enrolled in our study. Expressions of CircCDR1as, miR‐135b, and FIH‐1 were detected by qRT‐PCR in affected necrosis tissue and non‐affected normal tissue. Bone microvascular endothelial cells (BMEC) were isolated from six patients and treated with 0.1 mg/mL hydrocortisone to establish a GC‐damaged model of BMECs. Circ CDR1as plasmid and miR‐135b mimic were transfected into BMECs. BMEC proliferation was assessed using MTT assays. The migration ability of cells was detected by scratch‐wound assays. Matrigel assay was performed to detect angiogenesis in vitro. Western blot assay was used to detect HIF‐1α, VEGF, and FIH‐1 expressions. FISH, RNA pull down, RIP, and luciferase assay were carried out to determine the interaction of CircCDR1as, miR‐135b, and FIH‐1.ResultsCircCDR1as was upregulated(2.02 ± 0.30 vs. 1.00 ± 0.10,P < 0.001) whereas miR‐135b was downregulated (0.55 ± 0.12 vs. 1.00 ± 0.10,P < 0.001) in affected tissues than in non‐affected tissues. Expression of CircCDR1as and FIH‐1 were negatively associated with miR‐135b in affected tissues (CircCDR1as with miR‐135b: r = −0.506, P < 0.001; FIH‐1 with miR‐135b r = −0.510, P < 0.001). Total blood tubule density was increased when CircCDR1as was silenced compared with NC (P < 0.01 vs. NC). The number of migrated BMECs were significantly increased in CircCDR1as silencing group compared with NC group (P < 0.05 vs. NC). In addition, CircCDR1as plasmids transfection increased the protein expressions of FIH‐1 (P < 0.05 vs. NC) and reduced the HIF‐1α as well as VEGF expression compared with NC group (P < 0.05 vs. NC). FISH, RNA pull down, RIP, and luciferase assay identified that FIH‐1 was a target of miR‐135b and could be modulated by CircCDR1as.ConclusionCircCDR1as decreases angiogenesis and proliferation of BMECs by sponging miR‐135b and upregulate FIH‐1.     

2018年烟台市同期五起伤寒病原学研究及溯源分析

目的分析烟台市5起伤寒病原学特征及分子流行病学关联。方法2018年自烟台市5例伤寒确诊病例样本和流行病调查样本分离获得6株伤寒沙门菌菌株,病例发病时间自2018年5月26日至7月24日,分别分布于烟台牟平区水道镇、烟台蓬莱区登州街道、烟台龙口东莱街道、烟台牟平区文化街道、烟台芝罘区福莱山街道。采用常规细菌分离方法和XbaⅠ/BlnⅠ双酶切脉冲场凝胶电泳(PFGE)技术对伤寒杆菌进行溯源分析,同时开展viaB毒力基因检测和27种临床常用抗生素敏感试验对伤寒杆菌进行病原学研究。结果6株伤寒杆菌经PFGE-XbaⅠ和PFGE-BlnⅠ电泳分为4个PFGE模式,其中3株菌同源性100%;1株多耐药菌(外籍患者),1株单耐药菌(外省滞留史患者),1株完全敏感菌,同一PFGE模式的3株菌药敏表型一致,除对氨基糖甙类和喹诺酮类部分抗生素中介外,对其他抗生素均敏感;除外籍患者标本分离株viaB基因阴性外,其他菌viaB基因均阳性。结论烟台寒杆菌对临床常用抗生素敏感性良好,仍需重视伤寒散发病例发生和伤寒输入感染。     

腺苷脱氨酶抑制剂对氧反常大鼠心脏的保护作用

本文观察到腺苷脱氨酶抑制剂EHNA对离体灌流大鼠心脏氧反常性损伤有明显的作用,此外还发现在缺氧期(无氧灌流30分钟)EHNA也表现出明显的保护作用,心脏收缩幅度的降低和静息张力的升高均显著低于对照组。表明在缺氧期也可能有自由基的产生。     

偶联剂CGE（N）—R型处理前后牙本质的表面状态及其...

In order to research the states of dentine surface and dentine surface primed with coupling agent, we used microscope, transmission electron microscope (TEM) and solid fluorescence method. Observed under microscope, patterning of dentinal tubules and their poles was very explicit; through the view of interface between coupling agent adsorbed and human dentine powder by TEM, it was revealed that the coupling agent was adsorbed onto the dentine surface as if it "grew out" of the dentine surface; through examination of interface between the coupling agent adsorbed and dentine surface by fluorescence spectrum, which differed from that of the coupling agent or dentine surface, i.e., this fluorescence spectrum of interface changed obviously (red shift). This phenomenon indicated that there was a chemical reaction occurring between tooth surface and adsorbed coupling agent. Therefore we can conclude that the three examinations are necessary to provide the argument for establishment of chemical bonding mechanism between dentine and coupling agent.     

膀胱移形细胞癌微血管密度与病理组织学分级和预后的关系

目的：探讨肿瘤血管形成及其密度与膀胱移行细胞癌生物学行为的关系。方法：应用免疫组织化学方法检测了６７例膀胱移行细胞癌组织中ＦⅧ因子相关抗原的表达和肿瘤组织中微血管的长度密度（ＬＶ）和微血管截面数（ＱＡ）。结果：膀胱癌组织中微血管ＬＶ明显高于正常膀胱（Ｐ〈０．０１）。但高分化膀胱癌微血管ＬＶ与正常膀胱无显著性差异（Ｐ〉０．０５）。膀胱癌分化越差,微血管ＬＶ越高;有肌层浸润组微血管ＬＶ明显高于无肌层浸     

糖尿病患者血清中IL-4、TNF-a的检测及分析

目的 探讨白介素４（ＩＬ－４）、肿瘤坏死因子（ＴＮＦ－ａ）水平与糖尿病关系。方法 采用ＥＬＩＳＡ法测定５４例糖尿病患者及４０例健康人血清ＩＬ－４、ＴＮＦ－ａ含量。结果 糖尿病患者ＩＬ－４平均含量明显低于健康人（Ｐ〈０．０５）,且与血糖一负相关（ｒ＝－０．３４７,Ｐ〈０．０５）。ＴＮＦ－ａ含量略高于对照组,但无统计学意义。结论 糖尿病患者内存在细胞免疫功功能紊乱,自身免疫功能调节失衡影响糖尿病的发生     

The acceptance of SARS-CoV-2 rapid antigen self-testing: A cross-sectional study in China

Compared with the nucleic acid amplification test (NATT), the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rapid antigen self-testing (RAST) has advantages in speed and convenience. However, little is known about people's acceptance and influencing factors for SARS-CoV-2 RAST. A cross-sectional study was conducted from April 21 to 30, 2022 in China. The χ² test and multivariate logistic regressions were used to identify the influencing factors. The structural equation model was used to test the extended protective motivation theory (PMT) model hypotheses. Among the total of 5107 participants, 62.5% were willing to accept the SARS-CoV-2 RAST. There were significant differences in acceptance among different residences (p < 0.001), educational level (p < 0.001), occupation (p < 0.001), monthly income (p < 0.001), travel frequency (p < 0.05), and feelings about NATT (p < 0.001). Response efficacy (β = 0.05; p = 0.025) and self-efficacy (β = 0.84; p < 0.001) had a positive effect, while response cost showed a negative effect (β = −0.07; p < 0.001). The public's major concerns about SARS-CoV-2 RAST are its reliability, testing method, price, and authority. Overall, a moderate intention to use SARS-CoV-2 RAST was found among the Chinese population. The extended PMT can be used for the prediction of intention to accept the RAST. We need to take measures to increase people's acceptance of SARS-CoV-2 RAST.     

脐血临床应用前的实验研究

对５８例脐血红细胞进行了计数和血红蛋白含量测定,并测定乙肝两对半、丙肝抗体、人类免疫缺陷病毒抗体、肝功能及Ｔ细胞亚群分类和细菌培养。结果表明无传染病的正常孕妇分娩时的脐血,可作血源供临床使用并能用于治疗多种原因引起的贫血。     

获取母血中纯化胎儿有核红细胞行产前基因分析

目的　探讨获取母血中纯化胎儿细胞的有效方法。方法　对 6 0名孕龄 7～ 40周、年龄 2 1～ 30岁的孕妇外周血进行不连续密度梯度离心 ,将分离后的细胞进行制片 ,显微镜下行显微操作分离胎儿有核红细胞 ,进行 Y染色体特异性DYZ1基因的聚合酶链反应 (PCR)。结果　 (1)早孕期母血中胎儿 NRBC检出率为 2 0 % ,中孕期母血中胎儿 NRBC检出率为 71.4% ,晚孕期母血中胎儿 NRBC检出率为 6 .6 5 % ,三者之间有显著性差异 (P <0 .0 5 ) ;(2 )用显微操作技术获得的胎儿细胞数量已满足 PCR扩增所需的模板量 ,对胎儿性别进行预测 ,男胎符合率为 11/ 13(84.6 % ) ,性别符合率为 2 6 / 2 8(92 .9% )。结论　不连续密度梯度离心合并显微操作技术对于无创性早期产前基因诊断意义重大。     

血清尿酸的毛细管电泳测定

目的用毛细管电泳中的胶束动电法直接测定血清尿酸浓度。方法以ＳＤＳ作为电泳缓冲液中和胶束相,采用非涂渍毛细管２１ｃｍ×５０μｍ（ｉ．ｄ．）,检测波长２３５ｎｍ,以外标法定量。结果尿酸测定的线性范围为４６．５～１５００μｍｏｌ／Ｌ,最低检测限为２０．３１μｍｏｌ／Ｌ：本法的日内和日间变异系数均小于４．５％;平均回收率为１０１．４５％。内生性化合物和临床某些常用药物对此方法无干扰。结论该法线性范围宽,简单、快速,可应用于临床样品检测。