冠心病患者血清C-反应蛋白水平变化的研究

目的 :探讨 C-反应蛋白 ( CRP)与冠心病 ( CHD)的关系。方法 :观察 6 9例冠状动脉至少单支狭窄≥6 0 %的 CHD患者及 35例正常对照者的 C-反应蛋白 ( CRP)浓度及 CRP浓度的高低与冠状动脉病变程度的关系。结果 :CHD患者的 CRP浓度较正常对照组显著增高 ( P<0 .0 1 ) ,CRP浓度与冠状动脉病变程度呈正相关 ( P<0 .0 1 )。结论 :CRP浓度可作为评价冠状动脉病变及其严重程度的一个参考指标。     

内窥镜辅助下经单鼻腔蝶窦入路切除垂体腺瘤

目的　探讨局部麻醉下利用内窥镜辅助 ,经单鼻腔蝶窦入路切除垂体腺瘤的可行性、优缺点 ,适应证及注意事项。方法　采用MRI明确诊断 ,使用局部麻醉内窥镜电视监测下经单鼻腔蝶窦入路进行垂体腺瘤切除。结果　 30例病人中 2 5例肿瘤全切除 ,5例次全切除。术后 2～ 4小时病人即可下床活动 ,进食。术后平均 4天出院 ,无死亡、无感染。术后脑脊液鼻漏 1例 ,再次经鼻蝶入路修补后痊愈。随访 6～ 4 8个月 ,视力恢复到 1.0以上10例 ,内分泌功能正常 15例 ,月经恢复正常 10例。结论　内窥镜辅助下经单鼻腔切除垂体瘤使用局部麻醉是安全可行的 ,手术对病人的损伤和影响可以达到最低程度     

Identification of novel biomarkers differentially expressed between African-American and Caucasian-American prostate cancer patients

Prostate cancer (PCa) incidence and mortality rate vary among racial and ethnic groups with the highest occurrence in African American (AA) men who have mortality rates twice that of Caucasians (CA). In this study, we focused on differential expression of proteins in AA prostate cancer compared to CA using Protein Pathway Array Analysis (PPAA), in order to identify protein biomarkers associated with PCa racial disparity. Fresh frozen prostate samples (n=90) obtained from radical prostatectomy specimens with PCa, including 25 AA tumor, 21 AA benign, 23 CA tumor, 21 CA benign samples were analyzed. A total of 286 proteins and phosphoproteins were assessed using PPAA. By PPAA analysis, 33 proteins were found to be significantly differentially expressed in tumor tissue (n=48, including both CA and AA) in comparison to benign tissue (n=42). We further compared protein expression levels between AA and CA tumor groups and found that 3 proteins were differentially expressed (P<0.05 and q<5%). Aurora was found to be significantly increased in AA tumors, while Cyclin D1 and HNF-3a proteins were downregulated in AA tumors. Predicted risk score was significantly different between AA and CA ethnic groups using logistic regression analysis. In conclusion, we identified Aurora, Cyclin D1 and HNF-3a proteins as being differentially expressed between AA and CA in PCa tissue. Our study suggests that these proteins might be involved in different pathways that lead to aggressive PCa behavior in AA patients, potentially serving as biomarkers for the PCa racial disparity.     

病程和情绪障碍对不同运动症状发病部位帕金森病患者神经认知功能的影响

目的：探讨病程和情绪障碍对不同运动症状发病部位帕金森病（PD）患者认知功能的影响是否存在交互作用。方法纳入35例左侧起病的PD患者和31例右侧起病的PD患者,对所有患者进行成套情绪问题和认知功能评估,并探索其是否存在左右交互影响。结果病程和情绪是右侧起病的PD患者认知功能的重要预测因子（均P＜0.05）,但在左侧起病者无相关性（均P＞0.05）。病程与左侧起病的PD患者在视觉模拟情绪量表中的活力呈正相关（P＜0.05）。结论情绪问题和病程只交互影响右侧起病的运动症状PD患者的认知功能。     

人cGAS 基因启动子的克隆鉴定及功能初探

目的：构建人环鸟苷酸?腺苷酸合成酶（cyclic guanosine monophosphate?adenosine monophosphate synthase,cGAS）基因启动子,确认其活性,进而对转录调控机制进行初步探究。方法：采用 PCR 方法扩增人cGAS 基因 5′上游 1 254 bp（-1 178~+76 bp）的片段,酶切后连接到pGL3?basic 质粒,以该质粒为模板合成不同长度的启动子质粒。通过双荧光素酶报告基因实验验证不同质粒在Hela细胞中的活性,利用生物信息学方法预测启动子区存在的转录因子结合位点。通过点突变实验验证潜在的转录因子结合位点。结果：人cGAS启动子质粒初步构建成功。转染后,cGAS 启动子重组质粒的相对荧光素酶活性增加（P＜0.05）。人cGAS近端启动子区域（-414~+76 bp）经生物信息学软件分析可能含有 Sp1、CREB、USF1、RAP1、C?JUN、OCT?1等转录因子的结合位点。点突变实验证实Sp1、CREB正向调控该启动子区域。结论：人cGAS近端启动子区域（-414~+76 bp）存在较强的启动子活性,该区域含有多个潜在的转录因子结合位点。转录因子Sp1、CREB调控人cGAS启动子区。     

人FAT1基因启动子的克隆鉴定及其功能浅析

目的：克隆人脂肪非典型钙黏蛋白1（FAT atypical cadherin 1,FAT1）基因启动子,找到核心启动子区域并对其转录调控机制进行初步分析。方法：通过PCR方法获得人FAT1基因5′上游1 163 bp（-1 029~+134 bp）的片段,亚克隆至pGL3?basic载体;通过步移缺失构建不同缺失片段的重组质粒。双荧光素酶报告活性分析检测各重组质粒在A549细胞和HEK293T细胞中的活性,找到核心启动子区域。利用生物信息学方法预测核心区域的转录因子结合位点。结果：经测序、酶切鉴定,成功构建了人FAT1启动子荧光素酶活性报告基因重组质粒。与pGL3?basic质粒相比,人FAT1启动子重组质粒的相对荧光素酶活性增加（P < 0.05）。通过生物信息学软件预测人FAT1启动子区域（-233~-110 bp）可能含有TFAP2C、KLF5等转录因子结合位点。结论：成功构建人FAT1启动子不同缺失片段的荧光素酶报告基因重组质粒。通过荧光素酶活性比较,推测人FAT1的核心启动子区域位于-233~+134 bp区域,其中可能含有若干转录因子结合位点。